Microvirga indica sp. nov., an arsenite-oxidizing Alphaproteobacterium, isolated from metal industry waste soil

Tapase, Savita R.; Mawlankar, Rahul; Sundharam, Shiva S.; Krishnamurthi, Srinivasan; Dastager, Syed G.; Kodam, Kisan M.

doi:10.1099/ijsem.0.002157

Cited by 22 publications

(10 citation statements)

References 31 publications

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“…The genus Microvirga is a cosmopolitan genus which as of now includes 17 validated species isolated from a wide diversity of niches, such as human stool [9], air [10], thermal water [11], natural, domestic, and contaminated soils [12,13,14,15,16,17], and even as legume nodule endophytes [18,19,20]. Some studies report the genetic potential of some strains classified within the Microvirga genus for arsenic oxidation [15] and the production of pigments and amylolytic enzymes [21].…”

Section: Introductionmentioning

confidence: 99%

Genome Insights into the Novel Species Microvirga brassicacearum, a Rapeseed Endophyte with Biotechnological Potential

et al. 2019

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Plants harbor a diversity of microorganisms constituting the plant microbiome. Many bioinoculants for agricultural crops have been isolated from plants. Nevertheless, plants are an underexplored niche for the isolation of microorganisms with other biotechnological applications. As a part of a collection of canola endophytes, we isolated strain CDVBN77T. Its genome sequence shows not only plant growth-promoting (PGP) mechanisms, but also genetic machinery to produce secondary metabolites, with potential applications in the pharmaceutical industry, and to synthesize hydrolytic enzymes, with potential applications in biomass degradation industries. Phylogenetic analysis of the 16S rRNA gene of strain CDVBN77T shows that it belongs to the genus Microvirga, its closest related species being M. aerophila DSM 21344T (97.64% similarity) and M. flavescens c27j1T (97.50% similarity). It contains ubiquinone 10 as the predominant quinone, C19:0 cycloω8c and summed feature 8 as the major fatty acids, and phosphatidylcholine and phosphatidylethanolamine as the most abundant polar lipids. Its genomic DNA G+C content is 62.3 (mol %). Based on phylogenetic, chemotaxonomic, and phenotypic analyses, we suggest the classification of strain CDVBN77T within a new species of the genus Microvirga and propose the name Microvirga brassicacearum sp. nov. (type strain CDVBN77T = CECT 9905T = LMG 31419T).

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Section: Introductionmentioning

confidence: 99%

Genome Insights into the Novel Species Microvirga brassicacearum, a Rapeseed Endophyte with Biotechnological Potential

et al. 2019

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“…All strains (except BCRC 80972 T ) were negative for oxidase, Tween 80, α-galactosidase, β-galactosidase, β-glucuronidase, β-glucosidase, N-acetyl-β-glucosaminidase, α-mannosidase, β-fucosidase, lysine decarboxylase, ornithine decarboxylase, onitrophenyl-β-d-galactopyranoside, hydrogen sulfide, citrate, amygdalin, melibiose, sucrose, rhamnose, sorbitol, inositol, mannose, glucose, indole production, hydrolysis of starch, gelatin and degradation of agar. Data for strain BCRC 80972 T from reference [6]. +, Positive; -, negative; nd, not determined.…”

Section: Genomic Characterizationmentioning

confidence: 99%

Microvirga roseola sp. nov. and Microvirga lenta sp. nov., isolated from Taklamakan Desert soil

Wang

et al. 2022

International Journal of Systematic and Evolutionary Microbiology

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Two Gram-negative, rod-shaped, non-spore-forming bacteria, designated SM9T and SM2T, were isolated from Taklamakan Desert soil samples. Phylogenetic analysis based on the 16S rRNA gene sequences showed that strains SM9T and SM2T had the highest sequence similarity to the type strains Microvirga indica BCRC 80972T and Microvirga soli NBRC 112417T with similarity values of 98.2 and 97.7 %, respectively, and Microvirga was among the predominant genera in the desert soil. The draft genomes of these two strains were 4.56 Mbp (SM9T) and 5.08 Mbp (SM2T) long with 65.1 mol% (SM9T) and 63.5 mol% (SM2T) G+C content. To adapt to the desert environment, these two strains possessed pathways for the synthesis of stress metabolite trehalose. The major fatty acids (>5 %) included C18 : 1 ω9c in SM2T, but C16 : 0, C18 : 0 and C19 : 0 cyclo ω8c in SM9T, while the major menaquinone was ubiquinone 10 in both strains. The major polar lipids of SM9T and SM2T were phosphatidylglycerol, phosphatidylethanolamine and phospholipid. The average nucleotide identity and digital DNA–DNA hybridization results further indicated that strains SM9T and SM2T were distinguished from phylogenetically related species and represented two novel species within the genus Microvirga , for which the names Microvirga roseola sp. nov. (type strain SM2T=KCTC 72792T=CGMCC 1.17776T) and Microvirga lenta sp. nov. (type strain SM9T=KCTC 82729T=CCTCC AB 2021131T) are proposed.

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“…Accordingly, we carried out a programme to explore potential sources of psychrotolerant species from Antarctic soil, a putatively novel strain 3D7 T of the genus Microvirga was isolated. The genus Microvirga was proposed by Kanso classi ed within the Microvirga genus for arsenic oxidation (Tapase et al 2017) and the production of pigments, amylolytic enzymes (Radl et al 2017), phosphatases and exopolysaccharides (Jiménez- Gómez et al 2019). Based on polyphasic taxonomic characterisation, we propose the description of Microvirga antarctica sp.…”

Section: Introductionmentioning

confidence: 99%

Description and Genome Analysis of Microvirga Antarctica Sp. Nov., A Novel Pink-Pigmented Psychrotolerant Bacterium Isolated From Antarctic Soil

Zhu

Ping

Zhang

et al. 2021

Preprint

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During the investigation of exploring potential psychrotolerant species from Antarctica soil, a novel pink-pigmented bacterium designated strain 3D7T was isolated. Cells of the isolate were observed to be rod-shaped (0.7–0.9×1.0–2.2 µm), Gram-stain negative and non-motile. It was able to grow at 4–32 ℃, pH 7.0–10.0 and in the presence of 0–3 % (w/v) NaCl. Phylogenetic analysis based on 16S rRNA gene sequences showed that strain 3D7T belongs to the genus Microvirga and was most closely related to ‘Microvirga brassicacearum’ CDVBN77T (98.3 %), Microvirga subterranea DSM 14364T (96.8 %), Microvirga guangxiensis 25BT (96.5 %) and Microvirga aerophila DSM 21344T (96.5 %). The predominant quinone was ubiquinone 10 (Q-10), and the major fatty acids were summed feature 8 (C18:1ω7c and/or C18:1ω6c) and C19:0 cyclo ω8c. The predominant polar lipids were phosphatidylcholine and phosphatidylethanolamine. The genomic DNA G + C content of strain 3D7T was 63.5 mol%. Its genome sequence showed genes encoding phosphatases and lipases. Genetic machinery related to carbohydrate-active enzymes and secondary metabolites were also observed. The average nucleotide identity and digital DNA–DNA hybridization values based on whole genome sequences of strain 3D7T and its closely related species were below the threshold range for species determination. Phenotypic, chemotaxonomic, phylogenetic and genomic analyses suggested that strain 3D7T represents a novel species of the genus Microvirga, for which the name Microvirga antarctica sp. nov. is proposed. The type strain is 3D7T (= CGMCC 1.13821T = KCTC 72465T).

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Microvirga indica sp. nov., an arsenite-oxidizing Alphaproteobacterium, isolated from metal industry waste soil

Cited by 22 publications

References 31 publications

Genome Insights into the Novel Species Microvirga brassicacearum, a Rapeseed Endophyte with Biotechnological Potential

Genome Insights into the Novel Species Microvirga brassicacearum, a Rapeseed Endophyte with Biotechnological Potential

Microvirga roseola sp. nov. and Microvirga lenta sp. nov., isolated from Taklamakan Desert soil

Description and Genome Analysis of Microvirga Antarctica Sp. Nov., A Novel Pink-Pigmented Psychrotolerant Bacterium Isolated From Antarctic Soil

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