Microvesicles released from tumor cells disrupt epithelial cell morphology and contractility

Bordeleau, François; Chan, Bryan; Antonyak, Marc A.; Lampi, Marsha C.; Cerione, Richard A.; Reinhart‐King, Cynthia A.

doi:10.1016/j.jbiomech.2015.10.003

Cited by 16 publications

(11 citation statements)

References 55 publications

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“…This may also arise from the mesenchymal cell line used here (MDA-MB-231), which has been observed to secrete exosomes that reprogram epithelial cells (MCF-10A) to undergo EMT. 51, 52 Finally, co-cultured primary liver hepatocytes have been observed to reprogram mesenchymal cell line (MDA-MB-231) to undergo MET. 53 We see some less elongation and vimentin expression in co-cultured MDA-MB-231 cells, but additional measurements of epithelial biomarkers are necessary to establish conclusively that MET is occurring.…”

Section: Discussionmentioning

confidence: 99%

Clustering and jamming in epithelial–mesenchymal co-cultures

2016

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Collective behaviors emerge from coordinated cell-cell interactions during the morphogenesis of tissues and tumors. For instance, cells may display density-dependent phase transitions from a fluid-like “unjammed” phase to a solid-like “jammed” phase, while different cell types can “self-sort.” Here, we comprehensively track single cell dynamics in mixtures of sheet-forming epithelial cells and dispersed mesenchymal cells. We find that proliferating epithelial cells nucleate multicellular clusters that coarsen at a critical density, arresting migration and strengthening spatial velocity correlations. The addition of mesenchymal cells can slow cluster formation and coarsening, resulting in more dispersed individual cells with weak spatial velocity correlations. These behaviors have analogies with a jamming-unjamming transition, where the control parameters are cell density and mesenchymal fraction. This complex interplay of proliferation, clustering and correlated migration may have physical implications for understanding epithelial-mesenchymal interactions in development and disease.

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Section: Discussionmentioning

confidence: 99%

Clustering and jamming in epithelial–mesenchymal co-cultures

2016

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“…MVs released by cancer cells may contain various proteins that regulate immune responses, including chemokine receptors, proinflammatory cytokines such as IL-1 or IL-6 and cytokine receptors such as TNFR1 [320]. Cancer cells, by releasing MVs, can disrupt epithelial cell morphology and promote EMT leading to tumor progression [321]. The cargo of LOs differs from other vesicles and includes factors associated with tumor progression [322].…”

Section: Microvesicles and Oncosomesmentioning

confidence: 99%

Modeling of the immune response in the pathogenesis of solid tumors and its prognostic significance

2020

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Background Tumor initiation and subsequent progression are usually long-term processes, spread over time and conditioned by diverse aspects. Many cancers develop on the basis of chronic inflammation; however, despite dozens of years of research, little is known about the factors triggering neoplastic transformation under these conditions. Molecular characterization of both pathogenetic states, i.e., similarities and differences between chronic inflammation and cancer, is also poorly defined. The secretory activity of tumor cells may change the immunophenotype of immune cells and modify the extracellular microenvironment, which allows the bypass of host defense mechanisms and seems to have diagnostic and prognostic value. The phenomenon of immunosuppression is also present during chronic inflammation, and the development of cancer, due to its duration, predisposes patients to the promotion of chronic inflammation. The aim of our work was to discuss the above issues based on the latest scientific insights. A theoretical mechanism of cancer immunosuppression is also proposed. Conclusions Development of solid tumors may occur both during acute and chronic phases of inflammation. Differences in the regulation of immune responses between precancerous states and the cancers resulting from them emphasize the importance of immunosuppressive factors in oncogenesis. Cancer cells may, through their secretory activity and extracellular transport mechanisms, enhance deterioration of the immune system which, in turn, may have prognostic implications.

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“…Features of these MVs that support tumor growth and invasiveness are linked to interactions with the tumor microenvironment, where they can induce a tumor-supporting milieu [ 161 , 162 ]. Bordeleau et al have suggested that tumor-derived MVs could contribute to extracellular matrix reorganization by enhancing the contractility of non-malignant epithelial cells within the primary tumor and the metastatic sites [ 163 ]. Also, Taheri et al have demonstrated that glioma tumor-derived MVs can stimulate proliferative and metastatic gene expression in normal astrocytes within the tumor microenvironment, thus affecting tumor growth and invasion [ 164 ].…”

Section: Tumor-derived Mvsmentioning

confidence: 99%

Membrane Microvesicles as Potential Vaccine Candidates

Shkair

Garanina

Stott

et al. 2021

IJMS

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The prevention and control of infectious diseases is crucial to the maintenance and protection of social and public healthcare. The global impact of SARS-CoV-2 has demonstrated how outbreaks of emerging and re-emerging infections can lead to pandemics of significant public health and socio-economic burden. Vaccination is one of the most effective approaches to protect against infectious diseases, and to date, multiple vaccines have been successfully used to protect against and eradicate both viral and bacterial pathogens. The main criterion of vaccine efficacy is the induction of specific humoral and cellular immune responses, and it is well established that immunogenicity depends on the type of vaccine as well as the route of delivery. In addition, antigen delivery to immune organs and the site of injection can potentiate efficacy of the vaccine. In light of this, microvesicles have been suggested as potential vehicles for antigen delivery as they can carry various immunogenic molecules including proteins, nucleic acids and polysaccharides directly to target cells. In this review, we focus on the mechanisms of microvesicle biogenesis and the role of microvesicles in infectious diseases. Further, we discuss the application of microvesicles as a novel and effective vaccine delivery system.

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Microvesicles released from tumor cells disrupt epithelial cell morphology and contractility

Cited by 16 publications

References 55 publications

Clustering and jamming in epithelial–mesenchymal co-cultures

Clustering and jamming in epithelial–mesenchymal co-cultures

Modeling of the immune response in the pathogenesis of solid tumors and its prognostic significance

Membrane Microvesicles as Potential Vaccine Candidates

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