Microvesicles: mediators of extracellular communication during cancer progression

Muralidharan‐Chari, Vandhana; Clancy, James; Sedgwick, Alanna; D’Souza‐Schorey, Crislyn

doi:10.1242/jcs.064386

Cited by 817 publications

(793 citation statements)

References 93 publications

(110 reference statements)

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“…MiRNA-loaded exosomes shed from malignant cells can be transferred within the TME and are able to fuse with noninvolved cells, introducing the functional material they contain [137]. Similarly, microvesicles derived from immune cells can transfer pro-inflammatory mediators and produce local sites of inflammation that may contribute to tumorigenesis [138]. This cell-to-cell communication mediated via the transfer of miRNAs from tumor cells may facilitate the pre-malignant transformation of noninvolved cells in the TME.…”

Section: Emt and Mirna Regulationmentioning

confidence: 99%

The Inflammatory Tumor Microenvironment, Epithelial Mesenchymal Transition and Lung Carcinogenesis

Heinrich

Walser

Krysan

et al. 2011

Cancer Microenvironment

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The inflammatory tumor microenvironment (TME) has many roles in tumor progression and metastasis, including creation of a hypoxic environment, increased angiogenesis and invasion, changes in expression of microRNAs (miRNAs) and an increase in a stem cell phenotype. Each of these has an impact on epithelial mesenchymal transition (EMT), particularly through the downregulation of E-cadherin. Here we review seminal work and recent findings linking the role of inflammation in the TME, EMT and lung cancer initiation, progression and metastasis.Finally, we discuss the potential of targeting aspects of inflammation and EMT in cancer prevention and treatment.

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Section: Emt and Mirna Regulationmentioning

confidence: 99%

The Inflammatory Tumor Microenvironment, Epithelial Mesenchymal Transition and Lung Carcinogenesis

Heinrich

Walser

Krysan

et al. 2011

Cancer Microenvironment

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“…The pro‐regenerative properties of ASCs are explained by their paracrine and autocrine activities based on the secretion of membrane‐derived extracellular vesicles (ExMVs), which are known to play a critical role in intracellular signalling 15, 16. ExMVs were demonstrated to contain a broad range of growth factors, including vascular endothelial growth factors, fibroblast growth factors and transforming growth factor‐β—all of which are crucial in the treatment of MetS 17. Moreover, mesenchymal stem cells (MSCs) were shown to improve metabolic control in experimental models of type 2 diabetes (T2D), as measured by enhanced insulin secretion, improved insulin sensitivity and increased number of islet cells in the pancreas 18.…”

Section: Introductionmentioning

confidence: 99%

Combination of resveratrol and 5‐azacytydine improves osteogenesis of metabolic syndrome mesenchymal stem cells

Marycz

Kornicka

Irwin‐Houston³

et al. 2018

J Cellular Molecular Medi

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Endocrine disorders have become more and more frequently diagnosed in humans and animals. In horses, equine metabolic syndrome (EMS) is characterized by insulin resistance, hyperleptinemia, hyperinsulinemia, inflammation and usually by pathological obesity. Due to an increased inflammatory response in the adipose tissue, cytophysiological properties of adipose derived stem cells (ASC) have been impaired, which strongly limits their therapeutic potential. Excessive accumulation of reactive oxygen species, mitochondria deterioration and accelerated ageing of those cells affect their multipotency and restrict the effectiveness of the differentiation process. In the present study, we have treated ASC isolated from EMS individuals with a combination of 5‐azacytydine (AZA) and resveratrol (RES) in order to reverse their aged phenotype and enhance osteogenic differentiation. Using SEM and confocal microscope, cell morphology, matrix mineralization and mitochondrial dynamics were assessed. Furthermore, we investigated the expression of osteogenic‐related genes with RT‐PCR. We also investigated the role of autophagy during differentiation and silenced PARKIN expression with siRNA. Obtained results indicated that AZA/RES significantly enhanced early osteogenesis of ASC derived from EMS animals. Increased matrix mineralization, RUNX‐2, collagen type I and osteopontin levels were noted. Furthermore, we proved that AZA/RES exerts its beneficial effects by modulating autophagy and mitochondrial dynamics through PARKIN and RUNX‐2 activity.

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“…Important work has been devoted to the biology of tumor cell-derived MVs. Compelling evidence suggests that tumor cell-derived MVs have vital roles in tumor invasion and metastasis, and their pathological functions are still under intense investigation [11] . However, it…”

mentioning

confidence: 99%

Serum deprivation elevates the levels of microvesicles with different size distributions and selectively enriched proteins in human myeloma cells in vitro

et al. 2013

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Aim: To investigate the effects of serum deprivation (SD) on microvesicles (MVs) secreted from human myeloma cells and the implications for disease progression. Methods: RPMI 8226, U266, and KM3 human myeloma cells were incubated in medium containing 10% (non-SD) or 1% fetal bovine serum (SD) and MVs were isolated. The levels and size distribution of MVs were analyzed with flow cytometry. The protein profiles of MVs were studied using 2D SDS-PAGE, MALDI-TOF-MS, and Western blotting. NF-κB activation was analyzed using EMSA. Angiogenesis was examined in Eahy926 endothelial cells. Results: Exposure of RPMI 8226 cells to SD for 24 h did not alter the number of apoptotic cells. However, SD increased the number of MVs from RPMI 8226, U266, and KM3 cells to 2.5-, 4.3-, and 3.8-fold, respectively. The size distribution of SD MVs was also significantly different from that of non-SD MVs. Three proteins ZNF224, SARM, and COBL in SD MVs were found to be up-regulated, which were involved in cell cycle regulation, signal transduction and metabolism, respectively. Co-culture of SD MVs and RPMI 8226 cells increased NF-κB activation in the target RPMI 8226 cells. Furthermore, SD MVs from RPMI 8226 cells significantly increased the microtubule formation capacity of Eahy926 endothelial cells compared with non-SD MVs. Conclusion: SD elevates the levels of microvesicles with different size distribution and selectively enriched proteins in human myeloma cells in vitro. The selectively enriched proteins, especially ZNF224, may play key roles in regulation of myeloma cells, allowing better adaptation to SD.

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Microvesicles: mediators of extracellular communication during cancer progression

Cited by 817 publications

References 93 publications

The Inflammatory Tumor Microenvironment, Epithelial Mesenchymal Transition and Lung Carcinogenesis

The Inflammatory Tumor Microenvironment, Epithelial Mesenchymal Transition and Lung Carcinogenesis

Combination of resveratrol and 5‐azacytydine improves osteogenesis of metabolic syndrome mesenchymal stem cells

Serum deprivation elevates the levels of microvesicles with different size distributions and selectively enriched proteins in human myeloma cells in vitro

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