Microvesicles in Sepsis: Implications for the Activated Coagulation System

Lehner, Georg; Brandtner, Anna; Joannidis, Michael

doi:10.1007/978-3-319-51908-1_3

Cited by 2 publications

(2 citation statements)

References 48 publications

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“…Lehner, et al [42] stated that the source of plasma EVs could promote sepsis progression or resolution [16]. Specific proteins found in EVs usually indicate their tissue or organ's origin, which can be identified by proteomic approaches [19,20].…”

Section: Expression Profile Of Demirs In Septic Patientsmentioning

confidence: 99%

Extracellular Vesicle microRNA Biomarkers of Late Post-sepsis Survivors

Souza‐Siqueira¹,

Gritte²,

Weimann³

et al. 2023

Preprint

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Sepsis is a life-threatening condition with high hospital mortality. Elevated mortality has also been observed in patients after hospital discharge due to post-sepsis syndrome (PSS). The etiology of PSS is still not entirely known, but it involves inflammation. Plasma extracellular vesicles (EVs) are recognized as a unique mechanism of intercellular communication in inflammatory processes. It has been reported that EV microRNA (miRNA) production during the acute sepsis phase may persist until after disease resolution and is associated with PSS. We employed mass spectrometry and qPCR analysis to determine the protein and miRNA composition of plasma-derived EVs of 36 patients during sepsis-related hospitalization, immediately after ICU discharge (post-sepsis), and three, six, twelve, and up to 36 months post-sepsis. We determined that patients’ immune system cells were the primary EV source. Fifteen differentially expressed EV miRNAs (DEmiRs) were identified in samples from septic patients compared to the control group. Predictive analyses revealed that these DEmiRs could influence inflammation by modulating pathways mediated by NF-κB, STAT3, and TLR4 signaling activation. Thirteen miRNAs (-15b-5p,-16-5p,-20a-5p,-25-3p,-27a-3p,-29a-3p,-30d-5p,-93-5p,-146a-5p,-148a-3p,-191-5p,-195-5p,-223-3p) were downregulated in the death group compared to the survivor group, making them candidate prognostic markers of ICU survival. One year after ICU discharge, the expression of miR-21-5p and miR-195-5p were decreased in the survivor group. The miRNAs identified in the present study represent potential biomarkers for the survival prognosis of post-sepsis patients.

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Section: Expression Profile Of Demirs In Septic Patientsmentioning

confidence: 99%

Extracellular Vesicle microRNA Biomarkers of Late Post-sepsis Survivors

Souza‐Siqueira¹,

Gritte²,

Weimann³

et al. 2023

Preprint

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“…EVs are involved in severe pathophysiological events such as infections and/or inflammatory processes. The existing literature 74 implies that EVs play an important role in sepsis and septic shock. Given that both the pathogen and the host appear to rely on EVs as their tool of either attack or defense, it needs to be precisely deciphered which EVs populations mediate and promote progression of sepsis and which support its resolution and propel healing.…”

Section: Evs In Sepsis: a Friend Or Foe?mentioning

confidence: 99%

Extracellular Vesicles as Markers and Mediators in Sepsis

2018

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Sepsis is defined as life-threatening organ dysfunction caused by a dysregulated host response to infection. It remains a highly lethal condition in which current tools for early diagnosis and therapeutic decision-making are far from ideal. Extracellular vesicles (EVs), 30 nm to several micrometers in size, are released from cells upon activation and apoptosis and express membrane epitopes specific for their parental cells. Since their discovery two decades ago, their role as biomarkers and mediators in various diseases has been intensively studied. However, their potential importance in the sepsis syndrome has gained attention only recently. Sepsis and EVs are both complex fields in which standardization has long been overdue. In this review, several topics are discussed. First, we review current studies on EVs in septic patients with emphasis on their variable quality and clinical utility. Second, we discuss the diagnostic and therapeutic potential of EVs as well as their role as facilitators of cell communication via micro RNA and the relevance of micro-organism-derived EVs. Third, we give an overview over the potential beneficial but also detrimental roles of EVs in sepsis. Finally, we focus on the role of EVs in selected intensive care scenarios such as coagulopathy, mechanical ventilation and blood transfusion. Overall, the prospect for EV use in septic patients is bright, ranging from rapid and precise (point-of-care) diagnostics, prevention of harmful iatrogenic interventions, to using EVs as guides of individualized therapy. Before the above is achieved, however, the EV research field requires reliable standardization of the current methods and development of new analytical procedures that can close the existing technological gaps.

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Microvesicles in Sepsis: Implications for the Activated Coagulation System

Cited by 2 publications

References 48 publications

Extracellular Vesicle microRNA Biomarkers of Late Post-sepsis Survivors

Extracellular Vesicle microRNA Biomarkers of Late Post-sepsis Survivors

Extracellular Vesicles as Markers and Mediators in Sepsis

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