Microvascular permeability with sulfonylureas in normal and diabetic hamsters

Cyrino, Fátima Z. G. A.; Conde, Cristiane M.S.; Garcia, Adriana A.

doi:10.1016/s0026-0495(97)90313-9

Cited by 9 publications

(2 citation statements)

References 15 publications

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“…Because glibenclamide has been shown to possess antioxidant properties (30), it may also protect tissue against reperfusion injuries. In this regard, Bouskela and colleagues (31) have shown in a hamster model that glibenclamide improves tissue perfusion via a better microcirculatory blood flow. The potential reperfusion injury has obviously been ablated by the antioxidant properties of this pharmacologic agent.…”

Section: Discussionmentioning

confidence: 98%

The Atp-Sensitive Potassium-Channel Inhibitor Glibenclamide Improves Outcome in an Ovine Model of Hemorrhagic Shock

Maybauer¹,

Salsbury²,

Westphal³

et al. 2004

Shock

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This study was designed as a prospective laboratory experiment to evaluate the effects of the ATP-sensitive potassium-channel inhibitor glibenclamide on hemodynamics and end-organ function in an ovine model of hemorrhagic shock. Twenty-four adult sheep were anesthetized and surgically prepared to measure hemodynamics of the systemic and pulmonary circulation. The anterior surface of the abdominal aorta was exposed at a location 6 cm superior to the iliac bifurcation. After a 60-min period of stabilization, this location was punctured with a 14-G needle. To induce a hemorrhagic hypotension (mean arterial pressure [MAP] less than 50 mmHg) via bleeding, the needle was left in place for 15 s to insure good blood flow. Thereafter, it was removed, and the abdomen closed. The animals were then randomized to receive either glibenclamide (4 mg/kg over 15 min) or an equal volume of the vehicle, started 1 h postinjury. Hemodynamic variables were measured every 30 min. Compared with the control group, MAP and systemic vascular resistance index (SVRI) were significantly higher in the intervention group throughout the entire 6-h study period. Ileal pH and urine output were higher in treated than in control animals (4 h, ileal pH 7.29 +/- 0.31 vs. 7.17 +/- 0.6; 6 h, urine output 36 +/- 9 vs. 7.5 +/- 2 mL; P value less than 0.05 each). Because glibenclamide improved both hemodynamics and organ function, it may be a beneficial component in the acute treatment of hemorrhagic shock.

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Section: Discussionmentioning

confidence: 98%

The Atp-Sensitive Potassium-Channel Inhibitor Glibenclamide Improves Outcome in an Ovine Model of Hemorrhagic Shock

Maybauer¹,

Salsbury²,

Westphal³

et al. 2004

Shock

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“…Before the ischemic period, hamsters in which the prepared area showed spontaneous nonfading leaks or more than 10 fading leaks were discarded. The number of leaks was counted 10 min after the ischemic period because it was shown that the maximum number of leaks is observed at this time (9,14). …”

Section: Methodsmentioning

confidence: 99%

Gender differences in microcirculation: Observation using the hamster cheek pouch

Panazzolo¹,

Silva²,

Cyrino³

et al. 2013

Clinics

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OBJECTIVES:Estrogen has been shown to play an important protective role in non-reproductive systems, such as the cardiovascular system. Our aim was to observe gender differences in vivo with regard to the increase in macromolecular permeability and leukocyte-endothelium interaction induced by ischemia/reperfusion as well as in microvascular reactivity to vasoactive substances using the hamster cheek pouch preparation.METHODS:Thirty-six male and 36 female hamsters, 21 weeks old, were selected for this study, and their cheek pouches were prepared for intravital microscopy. An increase in the macromolecular permeability of post-capillary venules was quantified as a leakage of intravenously injected fluorescein-labeled dextran, and the leukocyte-endothelium interaction was measured as the number of fluorescent rolling leukocytes or leukocytes adherent to the venular wall, labeled with rhodamin G, during reperfusion after 30 min of local ischemia. For microvascular reactivity, the mean internal diameter of arterioles was evaluated after the topical application of different concentrations of two vasoconstrictors, phenylephrine (α1-agonist) and endothelin-1, and two vasodilators, acetylcholine (endothelial-dependent) and sodium nitroprusside (endothelial-independent).RESULTS:The increase in macromolecular permeability induced by ischemia/reperfusion was significantly lower in females compared with males [19 (17–22) leaks/cm2 vs. 124 (123–128) leaks/cm2, respectively, p<0.001), but the number of rolling or adherent leukocytes was not different between the groups. Phenylephrine-induced arteriolar constriction was significantly lower in females compared with males [77 (73–102)% vs. 64 (55–69)%, p<0.04], but there were no detectable differences in endothelin-1-dependent vasoreactivity. Additionally, arteriolar vasodilatation elicited by acetylcholine or sodium nitroprusside did not differ between the groups.CONCLUSION:The female gender could have a direct protective role in microvascular reactivity and the increase in macromolecular permeability induced by ischemia/reperfusion.

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Microvascular Permeability in Diabetes

Bottino

Tavares

2003

Molecular Basis for Microcirculatory Disorders

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Microvascular permeability with sulfonylureas in normal and diabetic hamsters

Cited by 9 publications

References 15 publications

The Atp-Sensitive Potassium-Channel Inhibitor Glibenclamide Improves Outcome in an Ovine Model of Hemorrhagic Shock

The Atp-Sensitive Potassium-Channel Inhibitor Glibenclamide Improves Outcome in an Ovine Model of Hemorrhagic Shock

Gender differences in microcirculation: Observation using the hamster cheek pouch

Microvascular Permeability in Diabetes

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