Microvascular integrity plays an important role for graft survival after experimental skin transplantation

Motsch, B.; Heim, Christian; Koch, N.; Ramsperger-Gleixner, M.; Weyand, Michael; Ensminger, Stephan

doi:10.1016/j.trim.2015.09.005

Cited by 6 publications

(5 citation statements)

References 41 publications

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“…Thus, the resulted scar tissue does not function like normal skin, as it does not contain the native skin structure such as hair follicles, sebaceous glands, and sensory nerve receptors 48. Although various approaches for improving skin graft survival and decreasing contraction have been tried 49-54, problems still exist in promoting early vascularization of grafts and alleviating inflammation 55, 56 to regenerate durable and functional skin. hMSCs have been employed for treatment of full thickness wounds in different animal models.…”

Section: Discussionmentioning

confidence: 99%

Pre-vascularization Enhances Therapeutic Effects of Human Mesenchymal Stem Cell Sheets in Full Thickness Skin Wound Repair

et al. 2017

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Split thickness skin graft (STSG) implantation is one of the standard therapies for full thickness wound repair when full thickness autologous skin grafts (FTG) or skin flap transplants are inapplicable. Combined transplantation of STSG with dermal substitute could enhance its therapeutic effects but the results remain unsatisfactory due to insufficient blood supply at early stages, which causes graft necrosis and fibrosis. Human mesenchymal stem cell (hMSC) sheets are capable of accelerating the wound healing process. We hypothesized that pre-vascularized hMSC sheets would further improve regeneration by providing more versatile angiogenic factors and pre-formed microvessels. In this work, in vitro cultured hMSC cell sheets (HCS) and pre-vascularized hMSC cell sheets (PHCS) were implanted in a rat full thickness skin wound model covered with an autologous STSG. Results demonstrated that the HCS and the PHCS implantations significantly reduced skin contraction and improved cosmetic appearance relative to the STSG control group. The PHCS group experienced the least hemorrhage and necrosis, and lowest inflammatory cell infiltration. It also induced the highest neovascularization in early stages, which established a robust blood micro-circulation to support grafts survival and tissue regeneration. Moreover, the PHCS grafts preserved the largest amount of skin appendages, including hair follicles and sebaceous glands, and developed the smallest epidermal thickness. The superior therapeutic effects seen in PHCS groups were attributed to the elevated presence of growth factors and cytokines in the pre-vascularized cell sheet, which exerted a beneficial paracrine signaling during wound repair. Hence, the strategy of combining STSG with PHCS implantation appears to be a promising approach in regenerative treatment of full thickness skin wounds.

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Section: Discussionmentioning

confidence: 99%

Pre-vascularization Enhances Therapeutic Effects of Human Mesenchymal Stem Cell Sheets in Full Thickness Skin Wound Repair

et al. 2017

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“…In another recent study by our group, clopidogrel alone or in combination with everolimus also showed protective effects on microvessels in a skin graft. 25 Preservation of these vessels might contribute to the inhibitory effect on the development of CAV. Both everolimus and clopidogrel are already approved for human therapy and have an established safety profile.…”

Section: Treatment Options Primarily Targeting Leukocytesmentioning

confidence: 99%

New Targets for the Prevention of Chronic Rejection after Thoracic Organ Transplantation

Heim

Gocht

Weyand

et al. 2017

Thorac cardiovasc Surg

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The gold standard for the treatment of terminal heart failure and irreversible lung diseases includes thoracic organ transplantation. The major obstacle for long-term survival after successful transplantation is chronic rejection, an ongoing immunomodulatory disease so far without effective therapy. Therefore, the aim of this review is to elucidate scientific efforts targeting different new mechanisms of cardiac allograft vasculopathy (CAV) and chronic lung allograft dysfunction (CLAD). For this purpose, we performed a systematic review of the literature to assess recent strategies in transplant immunology research. We searched MEDLINE from 2015 up to date for articles addressing the following keywords: CAV, transplant vasculopathy, transplant arteriosclerosis, CLAD, bronchiolitis obliterans transplant, and obliterative bronchiolitis transplant. All articles including experimental models in the field of transplant immunology addressing new aspects for the prevention of chronic rejection after heart and lung transplantation were included in this review. The prevention of chronic rejection would clearly improve the survival of patients after heart and lung transplantation. Interesting targets were addressed in recent research, but further research is necessary to effectively treat this life-threatening disease in transplant recipients.

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“…This requires extensive research on the wound-healing process and related factors [ 3 ]. Skin grafting effectively treats burns as it covers the affected area and allows for regeneration [ 3 , 4 ].…”

Section: Introductionmentioning

confidence: 99%

Skin graft surgery and its impact on platelet counts in Iranian burn patients: a non-randomized clinical trial

Kazemzadeh,

Pakzad,

Parizad

et al. 2024

BMC Surg

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Background Platelets are critical in maintaining homeostasis and immune response in burn patients. The concentration of platelets decreases in burn patients, and any intervention that increases serum platelet concentration can prevent serious consequences and patient death. The present study aimed to assess the impact of skin graft surgery on burn patients’ platelet counts. Methods In this non-randomized clinical trial, 200 burn patients were investigated. The patients were recruited from the surgical ward of Imam Khomeini Teaching Hospital during the first six months of 2021. After completing the checklist, patients underwent skin graft surgery. Blood was taken from the patients during surgery in the operating room and on the third and fifth day after the surgery to check platelets. Data analysis was conducted using SPSS software (ver. 22.0). Results Most patients (63.5%) were male, and 73 (36.5%) were female. One hundred eighty-one patients (90.5%) had deep burns, and 19 (9.5%) had superficial burns. The mean burns percentage in the patients was 19.3 ± 15.4%, the lowest was 2%, and the highest was 90%. The most common burns were caused by flame (42%) and boiling water (30.5%). The patients’ outcomes revealed that 6% gained complete recovery, 86.5% partial recovery, 2.5% showed transplant rejection, and 5% died. Mean platelet levels in deceased patients had an upward trend. The mean platelet counts of patients were elevated during surgery (289,855 ± 165,378), decreased three days after surgery (282,778 ± 317,310), and elevated again five days after surgery (330,375 ± 208,571). However, no significant difference was found between the mean platelet counts during surgery, the third and fifth days after surgery in patients undergoing skin grafts (P = 0.057). Conclusions This study suggests that skin graft positively increases the patient’s platelets. Further studies are needed to confirm the findings and elucidate the mechanism. Iranian Registry of Clinical Trial approval code (IRCT# IRCT20131112015390N8 & 06/01/2024).

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Microvascular integrity plays an important role for graft survival after experimental skin transplantation

Cited by 6 publications

References 41 publications

Pre-vascularization Enhances Therapeutic Effects of Human Mesenchymal Stem Cell Sheets in Full Thickness Skin Wound Repair

Pre-vascularization Enhances Therapeutic Effects of Human Mesenchymal Stem Cell Sheets in Full Thickness Skin Wound Repair

New Targets for the Prevention of Chronic Rejection after Thoracic Organ Transplantation

Skin graft surgery and its impact on platelet counts in Iranian burn patients: a non-randomized clinical trial

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