Microvascular Endothelial Cells from Brain

Bowman, Phillip D.; Bois, Monica Du; Dorovini-Zis, K.; Shivers, Richard R.

doi:10.1007/978-3-642-75262-9_10

Cited by 10 publications

(3 citation statements)

References 50 publications

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“…Ultrastructural observation by electron microscopy disclosed that there were few, if any, Weibel-Palade bodies (Fig. 1C); and this fact is con sistent with previous reports (22,23) which had shown a similar result with microvascular endothelial cells from brain and other organs. As shown in Fig.…”

Section: Identification Of Cultured Cellssupporting

confidence: 90%

Production of Platelet-Activating Factor by Porcine Brain Microvascular Endothelial Cells in Culture

et al. 1995

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SummaryEndothelial cells produce platelet-activating factor (PAF), which is the key process in the interactions between the vascular wall and blood cells. To examine the production of PAF in brain microvasculature we have cultured brain endothelial cells and performed a comparative study with aortic endothelial cells. Fresh porcine brain was homogenized, and microvascular endothelial cells were separated by enzyme digestion. The cells were cultured in medium containing epidermal growth factor and bovine brain extract. Endothelial cells from the aorta of the same animal were cultured in a similar manner. Production of PAF was assessed by ǀ3Hǀacetate incorporation into phospholipids or by radioimmunoassay. Prostacyclin was measured by radioimmunoassay of 6-ketoprostaglandin F1α. The cells produced 1760 ± 403 and 2892 ± 347 dpm/106 cells (n = 4) of PAF when stimulated with brady- kinin and calcium ionophore A23187, each at 1 μM, respectively. Aortic endothelial cells produced 3911 ± 2006 and 8052 ± 2270 dpm/106 cells (n = 4), respectively, and these values were significantly higher than those in brain endothelial cells (p<0.01, U-test). Prostacyclin production was also higher in aortic cells as compared to brain microvascular endothelial cells. In aortic endothelial cells both Ca ionophore A23187 and bradykinin significantly stimulated PMN adherence whereas in brain microvascular cells only Ca ionophore enhanced the adherence. Brain microvascular endothelial cells produce smaller amount of PAF and prostacyclin as compared to aortic endothelial cells, and this fact may imply that the functional integrity of the brain microvascular endothelium is maintained at a low level.

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Section: Identification Of Cultured Cellssupporting

confidence: 90%

Production of Platelet-Activating Factor by Porcine Brain Microvascular Endothelial Cells in Culture

et al. 1995

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“…After a period of 30 min the excess fibronectin is removed and BBEC that have been thawed and washed with culture media are seeded onto the growing surfaces at a density of 50 000 cells/cm 2. It should be noted that other laboratories have reported successful culturing of brain microvasculature endothelial cells using commercially available extracellular matrices (36) or fibronectin alone (18). For the first 3 days, the BBEC are cultured in MEM:F12 supplemented with 50 #g/ml polymixin B and 100 #g/ml heparin sulfate, to reduce microbial contamination and to promote endothelial cell growth, respectively.…”

Section: Isolation Of Brain Endothelial Cellsmentioning

confidence: 99%

“…To gain a better understanding of the BBB at the cellular level, an in vitro model using cultured brain microvasculature endothelial cells has been developed (4,5,18,31). Such a model allows for the mechanistic study of the metabolic and transport systems present in brain capillary endothelial cells, as well as the factors that influence or modify permeability in these cells.…”

Section: Introductionmentioning

confidence: 99%

Application of cultured endothelial cells of the brain microvasculature in the study of the blood-brain barrier

Miller

Audus

Borchardt

1992

Journal of Tissue Culture Methods

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SUMMARY: The endothelial cells of the brain microvasculature provide both physical and enzymatic barriers to the exchange of molecules between the extracellular fluid environment of the brain and the systemic circulation. To better understand these barrier properties and the factors that influence them at the cellular level, an in vitro model of the blood-brain barrier has been developed using primary cultures of bovine brain microvessel endothelial cells. The focus of the present paper is on the procedures associated with the isolation, culturing, and maintenance of bovine brain microvessel endothelial cells. Experimental applications of the cell culture model in studying the blood-brain barrier and efforts directed at improving the current cell culture model are also discussed.

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Production of Platelet-Activating Factor by Brain Microvascular Endothelial Cells

Satoh

Koyama

Yoshida

et al. 1996

Advances in Experimental Medicine and Biology

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Microvascular Endothelial Cells from Brain

Cited by 10 publications

References 50 publications

Production of Platelet-Activating Factor by Porcine Brain Microvascular Endothelial Cells in Culture

Production of Platelet-Activating Factor by Porcine Brain Microvascular Endothelial Cells in Culture

Application of cultured endothelial cells of the brain microvasculature in the study of the blood-brain barrier

Production of Platelet-Activating Factor by Brain Microvascular Endothelial Cells

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