Microvascular endothelial cell spreading and proliferation on nanofibrous scaffolds by polymer blends with enhanced wettability

Moffa, Maria; Polini, Alessandro; Sciancalepore, Anna Giovanna; Persano, Luana; Mele, Elisa; Passione, Laura Gioia; Potente, Giovanni; Pisignano, Dario

doi:10.1039/c3sm50328c

Cited by 38 publications

(30 citation statements)

References 64 publications

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“…As cell adhesion starts with recognition steps addressing the outermost surface of the scaffold, the design of successful biomimetic materials might require to functionalize fibers with motifs ensuring correct integrin binding . As schematized in Figure A, PLLA fibers were initially treated by oxygen plasma to improve their wetting properties . This process generates surface polar groups suitable for the subsequent covalent binding of functionalizing molecules .…”

Section: Resultsmentioning

confidence: 99%

“…C 3 ‐samples were also analyzed by FTIR spectroscopy (Figure E). PLLA fibers show characteristic absorption bands from C = O stretching vibrations in the carbonyl group at 1758 cm −1 , and bands at 1185 cm −1 and 1088 cm −1 , attributable to backbone ester groups . Peptide‐coated fibers also show two broad peaks in the amide N–H stretch region (3400–3600 cm −1 ) and amide I (1600–1700 cm −1 ), indicative of protein functionalization.…”

Section: Resultsmentioning

confidence: 99%

“…[ 7,58 ] As schematized in Figure 3 A, PLLA fi bers were initially treated by oxygen plasma to improve their wetting properties. [ 11 ] This process generates surface polar groups suitable for the subsequent covalent binding of functionalizing molecules. [ 59,60 ] Various peptide sequences present in ECM proteins might promote the affi nity of specifi c cell types.…”

Section: Immunofl Uorescence Assaysmentioning

confidence: 99%

“…Notwithstanding the numerous advantages of as‐produced, NF‐based materials, various postprocessing techniques applied to electrospun filaments have been demonstrated to significantly improve their performances for cell culturing . Pristine NFs made of synthetic polymers often exhibit a hydrophobic surface and lack cell‐binding motifs, therefore they benefit from grafted polar moieties and bioactive molecules to create a more favorable microenvironment for cell adhesion . A recent and very promising approach consists in linking small peptides or recombinant protein fragments containing sequences recognized by the integrin receptors .…”

Section: Introductionmentioning

confidence: 99%

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Bioactive Nanofiber Matrices Functionalized with Fibronectin‐Mimetic Peptides Driving the Alignment and Tubular Commitment of Adult Renal Stem Cells

Sciancalepore

Moffa

Carluccio

et al. 2015

Macro Chemistry & Physics

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Bioactive, electrospun polymer nanofi bers (NFs) are attractive building-block materials for realizing scaffolds for tissue engineering, as these fi laments structurally mimic the hierarchical organization of natural extracellular matrix. As presented in this article, demonstrated applications involve the commitment to adipogenic, chondrogenic, osteogenic, neural lineages, and so on, by different types of stem cells obtained from various sources (adult, embryonic, induced pluripotent stem cells, etc.). Since a poor cellular colonization may limit the usefulness of scaffolds for culturing delicate stem cell populations, coupling NFs with cell-adhesive domains is highly desirable. In our work, the surface of either isotropic (randomly oriented) or anisotropic (highly aligned) electrospun poly( L -lactic acid) fi brous scaffolds with fi bronectin (FN)-mimetic peptides is tailored. The effects of the peptide immobilization on the adhesion and spreading of human, adult renal stem cells are investigated. Functionalized, aligned NFs are suitable scaffolds to promote the confl uent growth of stem cells and to enhance their differentiation toward the tubular epithelial lineage. It is anticipated that the functionalization of aligned NFs with FN-mimetic peptides might be a promising method to target a specifi c response of stem cells in renal tissue engineering.

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Section: Resultsmentioning

confidence: 99%

Section: Resultsmentioning

confidence: 99%

Section: Immunofl Uorescence Assaysmentioning

confidence: 99%

Section: Introductionmentioning

confidence: 99%

See 2 more Smart Citations

Bioactive Nanofiber Matrices Functionalized with Fibronectin‐Mimetic Peptides Driving the Alignment and Tubular Commitment of Adult Renal Stem Cells

Sciancalepore

Moffa

Carluccio

et al. 2015

Macro Chemistry & Physics

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“…Spontaneous vascular ingrowth is approximately several tenths of a micrometer per day [22]. With respect to the current state of knowledge, complete vascularization of several-millimeters-thick scaffold takes a couple of weeks.…”

Section: Discussionmentioning

confidence: 99%

Vascularization Potential of Electrospun Poly(L-Lactide-co-Caprolactone) Scaffold: The Impact for Tissue Engineering

et al. 2017

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BackgroundElectrospun nanofibers have widespread putative applications in the field of regenerative medicine and tissue engineering. When compared to naturally occurring collagen matrices, electrospun nanofiber scaffolds have two distinct advantages: they do not induce a foreign body reaction and they are not at risk for biological contamination. However, the exact substrate, structure, and production methods have yet to be defined.Material/MethodsIn the current study, tubular-shaped poly(L-lactide-co-caprolactone) (PLCL) constructs produced using electrospinning technology were evaluated for their potential application in the field of tissue regeneration in two separate anatomic locations: the skin and the abdomen. The constructs were designed to have an internal diameter of 3 mm and thickness of 200 μm. Using a rodent model, 20 PLCL tubular constructs were surgically implanted in the abdominal cavity and subcutaneously. The constructs were then evaluated histologically using electron microscopy at 6 weeks post-implantation.ResultsHistological evaluation and analysis using scanning electron microscopy showed that pure scaffolds by themselves were able to induce angiogenesis after implantation in the rat model. Vascularization was observed in both tested groups; however, better results were obtained after intraperitoneal implantation. Formation of more and larger vessels that migrated inside the scaffold was observed after implantation into the peritoneum. In this group no evidence of inflammation and better integration of scaffold with host tissue were noticed. Subcutaneous implantation resulted in more fibrotic reaction, and differences in cell morphology were also observed between the two tested groups.ConclusionsThis study provides a standardized evaluation of a PLCL conduit structure in two different anatomic locations, demonstrating the excellent ability of the structure to achieve vascularization. Functional, histological, and mechanical data clearly indicate prospective clinical utilization of PLCL in critical size defect regeneration.

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Free poly(l-lactic acid) spherulites grown from thermally induced phase separation and crystallization kinetics

Liu

et al. 2014

J. Polym. Sci. Part B: Polym. Phys.

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Free poly(L-lactic acid) (PLLA) sheaves and spherulites were prepared by thermally induced phase separation method from its tetrahydrofuran solution without the assistance of other additives. The effects of variables such as polymer concentration, quenching temperature and time on the morphology of PLLA spherulites were studied. The morphology, size, degree of crystallinity, and crystal structure of spherulites were characterized by SEM, DSC and XRD, and so forth. No obvious sheaves or spherulites were observed at quenching temperature of 8 and 0 C, whereas sheaves composed of fluffy nanofibers with diameter of about 250 nm were formed at quenching temperature range of 210 to 240 C. With increasing quenching time, the PLLA morphology changed from small sheaves to big sheaves (cauliflower-like) to spherulites. Low concentration (3 and 5 wt %) solutions were favorable for the formation of sheaves, whereas high concentration (7 wt %) solution as good for the formation of spherulites. The mechanism for the formation of PLLA sheaves or spherulites was examined by the isothermal and nonisothermal crystalliza-Poly (L-lactic acid) (PLLA) has been widely used in the biomedical field. 7-10 It has many advantages of biocompatibility, hydrophilicity, and controllable degradation for clinical applications. Further, PLLA is a semicrystalline polymer. There are many reports on PLLA spherulites from its melt. For example, Miyata et al. 11 reported the growth characteristics of PLLA spherulites at various cooling rates from 200 C to room temperature. Cho et al. 12 studied several peculiarities of PLLA spherulites prepared at high crystallization temperatures above 130 C. There were a few reports on the PLLA crystallization from solution. Single crystals of PLLA were formed from 0.05 wt % PLLA-g-PCL/hexyl alcohol solution system at 50-80 C. 13 The rhombic and stelliform crystal of PLLA also were formed from 0.08 or 0.01 wt % PLLA/ acetonitrile solution system at 25 C. 14 However, there are few reports on PLLA spherulites from solution at low temperature.Thermally induced phase separation (TIPS) process was widely used to fabricate porous materials. This technique is Additional Supporting Information may be found in the online version of this article.

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Microvascular endothelial cell spreading and proliferation on nanofibrous scaffolds by polymer blends with enhanced wettability

Cited by 38 publications

References 64 publications

Bioactive Nanofiber Matrices Functionalized with Fibronectin‐Mimetic Peptides Driving the Alignment and Tubular Commitment of Adult Renal Stem Cells

Bioactive Nanofiber Matrices Functionalized with Fibronectin‐Mimetic Peptides Driving the Alignment and Tubular Commitment of Adult Renal Stem Cells

Vascularization Potential of Electrospun Poly(L-Lactide-co-Caprolactone) Scaffold: The Impact for Tissue Engineering

Free poly(l-lactic acid) spherulites grown from thermally induced phase separation and crystallization kinetics

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