Microvascular density and immunohistochemical expression of VEGF, VEGFR-1 and VEGFR-2 in benign prostatic hyperplasia, high-grade prostate intraepithelial neoplasia and prostate cancer

Abstract

IntroductionThe aim of our study was to determine and compare angiogenesis in benign prostatic hyperplasia (BPH), high-grade prostate intraepithelial neoplasia (HGPIN) and prostate cancer (Pca). Moreover, we evaluated its role as a prognostic factor for Pca.Material and methodsWe examined 39, 12 and 51 samples of BPH, HGPIN and Pca, respectively. Immunohistochemical methods were applied in order to evaluate the expression of VEGF and its receptors (VEGFR-1, VEGFR-2), while microvascular density (MVD) was determined using CD105. In Pca samples, we recorded stage, differentiation, perineural invasion, adjuvant radiotherapy and their correlation with angiogenesis.Results225 The expression of VEGF, VEGFR-1 and VEGFR-2 was significantly higher in Pca than compared to BPH (p <0.001, p <0.001 and p <0.001, respectively) and HGPIN (p <0.001, p <0.001 and p = 0.04, respectively), while there was no difference between BPH and HGPIN. MVD was higher in Pca compared to BPH (p <0.001) and HGPIN (p <0.01), while there was no difference between BPH and HGPIN. VEGF expression and MVD were significantly greater in Pca samples with poor differentiation (p = 0.044 and p = 0.038, respectively) and perineural invasion (p <0.001 and p = 0.019, respectively), while overexpression of VEGF was associated with advanced pathological stage (p = 0.047).ConclusionsAngiogenesis is more prominent in Pca than in BPH and HGPIN, while there is no difference between BPH and HGPIN. Pharmaceutical inhibition of angiogenesis could be a valuable therapeutic option for Pca in the near future.

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“…У кроликов при хронической ишемии ПЖ развивается стромальный фиброз и кистозная атрофия концевых секреторных отделов [17]. Железистая гипертрофия ПЖ, индуцированная нарушением кровоснабжения (ишемией), может быть взаимосвязана со снижением выработки сосудистого эндотелиального фактора роста (VEGF) [18,19].…”

“…21 2 0 2 0 ANDROLOGY AND GENITAL SURGERY О р и г и н а л ь н а я с т а т ь я 52 [17]. Glandular prostatic hypertrophy induced by blood supply disorders (ischemia) may be associated with decreased production of vascular endothelial growth factor (VEGF) [18,19].…”

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