Microvalve bioprinting as a biofabrication tool to decipher tumor and endothelial cell crosstalk: Application to a simplified glioblastoma model

Dusserre, Nathalie; Stachowicz, Marie-Laure; Médina, Chantal; Henri, Baptiste; Fricain, Jean-Christophe; Pâris, François; Oliveira, Hugo

doi:10.1016/j.bprint.2021.e00178

Cited by 6 publications

(12 citation statements)

References 39 publications

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“…Previous work has created in vitro models of this peroxide generation to assess the cytoprotective effect. 59 Inspired by these reports, we aimed to measure the cytoprotective effect of nanoceria against peroxides. Here, ADSCs were embedded in microgel suspensions and subjected to 24 hours of culture with varied concentrations of hydrogen peroxide (0, 100, and 1000 μM).…”

Section: Resultsmentioning

confidence: 99%

Gas modulating microcapsules for spatiotemporal control of hypoxia

Molley

Jiang

Kopecky

et al. 2022

Preprint

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Oxygen is a vital molecule involved in regulating development, homeostasis, and disease. The oxygen levels in tissue vary from 1 to 8% with deviations having major biological consequences. In this work, we developed an approach to encapsulate enzymes and nanozymes, at an unprecedented loading capacity, which precisely controls the oxygen content in cell culture. Here, a single microcapsule is able to locally perturb the oxygen balance, and varying the concentration and distribution of matrix embedded microcapsules provides spatiotemporal control. We demonstrate attenuation of hypoxia signaling in populations of stem cells, cancer cells, endothelial cells, and cancer spheroids. Capsule containing hydrogel films applied to chick chorioallantoic membranes encourage neovascularization, providing scope for topical treatments or hydrogel wound dressings. We further demonstrate versatility by loading capsules with ceria nanorods as nanozymes to modulate active oxygen species with potential as a cytoprotective treatment. The approach can be used in multiple formats, including deposition in hydrogels, as granular solids for 3D bioprinting, and as injectable biomaterials. Overall, this platform's simplicity and flexibility will prove useful for fundamental studies of oxygen-mediated processes in virtually any in vitro or in vivo format, with scope for inclusion in biomedical materials where controlling hypoxia may be clinically advantageous.

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Section: Resultsmentioning

confidence: 99%

Gas modulating microcapsules for spatiotemporal control of hypoxia

Molley

Jiang

Kopecky

et al. 2022

Preprint

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“…However, the U-87 MG GBM cell line was demonstrated to possess a different genetic profile from GBM . Other widely employed cell lines are U118-MG − and U251-MG. , …”

Section: Key Parametersmentioning

confidence: 99%

Applications of 3D Bioprinting Technology to Brain Cells and Brain Tumor Models: Special Emphasis to Glioblastoma

Ozbek,

Saybasili,

Ulgen

2024

ACS Biomater. Sci. Eng.

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Primary brain tumor is one of the most fatal diseases. The most malignant type among them, glioblastoma (GBM), has low survival rates. Standard treatments reduce the life quality of patients due to serious side effects. Tumor aggressiveness and the unique structure of the brain render the removal of tumors and the development of new therapies challenging. To elucidate the characteristics of brain tumors and examine their response to drugs, realistic systems that mimic the tumor environment and cellular crosstalk are desperately needed. In the past decade, 3D GBM models have been presented as excellent platforms as they allowed the investigation of the phenotypes of GBM and testing innovative therapeutic strategies. In that scope, 3D bioprinting technology offers utilities such as fabricating realistic 3D bioprinted structures in a layer-by-layer manner and precisely controlled deposition of materials and cells, and they can be integrated with other technologies like the microfluidics approach. This Review covers studies that investigated 3D bioprinted brain tumor models, especially GBM using 3D bioprinting techniques and essential parameters that affect the result and quality of the study like frequently used cells, the type and physical characteristics of hydrogel, bioprinting conditions, cross-linking methods, and characterization techniques.

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“…The next most common cell lines are (i) U118-MG, derived from a 50-year-old male patient with grade IV GBM; 13,14,81 and U251-MG, derived from a malignant GBM tumor. 82 Although less common, mouse glioblastoma cells (GL261) have been used to study the crosstalk between GBM cells and GBM-associated macrophages which were also derived from mouse. 16,31,79,[83][84][85] In this case, since the macrophages were mouse-derived, it was most ideal to use a mouse-derived GBM cell line rather than a human cell line.…”

Section: Colorectal Cancer (Crc)mentioning

confidence: 99%

“…79 Most multicellular models also include endothelial cells such as human umbilical vein endothelial cells (HUVECs) for vascularization since without this, the model would lack angiogenesis which is a critical characteristic of cancer. 18,79,82,85 Vascularization was assessed by staining the printed tissues with anti-CD31 which is a protein that is expressed in intercellular junctions when cells form capillary tubes. Finally, some studies incorporate stromal cells, microglia, and/or healthy astrocytes into their multicellular 3D models.…”

Section: Colorectal Cancer (Crc)mentioning

confidence: 99%