Microvalve-based bioprinting – process, bio-inks and applications

Ng, Wei Long; Lee, Jia Min; Yeong, Wai Yee; Naing, May Win

doi:10.1039/c6bm00861e

Cited by 183 publications

(133 citation statements)

References 116 publications

(206 reference statements)

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“…It can create complex 3D microstructures in programmed action and does not require sacrificial materials for mechanical support. Interestingly, external stimulation can be introduced in 3D printing device to reorganize the microstructures in micro and nano scales . For example, magnetic force was used to control the orientation of anisotropic particles in polymer ink during 3D printing .…”

Section: Conclusion and Perspectivementioning

confidence: 99%

3D Bioprinting of Artificial Tissues: Construction of Biomimetic Microstructures

Luo

Lin

Huang

2018

Macromolecular Bioscience

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It is promising that artificial tissues/organs for clinical application can be produced via 3D bioprinting of living cells and biomaterials. The construction of microstructures biomimicking native tissues is crucially important to create artificial tissues with biological functions. For instance, the fabrication of vessel-like networks to supply cells with initial nutrient and oxygen, and the arrangement of multiple types of cells for creating lamellar/complex tissues through 3D bioprinting are widely reported. The current advances in 3D bioprinting of artificial tissues from the view of construction of biomimetic microstructures, especially the fabrication of lamellar, vascular, and complex structures are summarized. In the end, the conclusion and perspective of 3D bioprinting for clinical applications are elaborated.

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Section: Conclusion and Perspectivementioning

confidence: 99%

3D Bioprinting of Artificial Tissues: Construction of Biomimetic Microstructures

Luo

Lin

Huang

2018

Macromolecular Bioscience

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“…However, none of the existing 3D bioprinters have been designed specifically for producing 3D cell cultures and as such are not ideal for this application. Inkjet printing drop-on-demand technologies have high cell compatibility and has been used extensively to print geometrically simple hydrogel constructs (20,21). Due to the droplet ejection mechanism, this technology shows very high cell viability but is limited to printing low viscosity bioinks (22) which effectively means bioinks with low cell concentration whereas high cell loadings are required for forming most 3D cell cultures.…”

Section: Introductionmentioning

confidence: 99%

Precise, high-throughput production of multicellular spheroids with a bespoke 3D bioprinter

Utama

Atapattu

O’Mahony³

et al. 2020

Preprint

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KeywordsBioprinting | 3d cell culture | high-throughput screening | multicellular spheroids | biomaterials Abstract 3D in vitro cancer models are important therapeutic and biological discovery tools, yet formation of multicellular spheroids in a throughput and highly controlled manner to achieve robust and statistically relevant data, remains challenging. Here, we developed an enabling technology consisting of a bespoke drop-on-demand 3D bioprinter capable of high-throughput printing of 96well plates of spheroids. 3D-multicellular spheroids are embedded inside a tissue-like matrix with precise control over size and cell number. Application of 3D bioprinting for high-throughput drug screening was demonstrated with doxorubicin. Measurements showed that IC50 values were sensitive to spheroid size, embedding and how spheroids conform to the embedding, revealing parameters shaping biological responses in these models. Our study demonstrates the potential of 3D bioprinting as a robust high-throughput platform to screen biological and therapeutic parameters. Significance StatementIn vitro 3D cell cultures serve as more realistic models, compared to 2D cell culture, for understanding diverse biology and for drug discovery. Preparing 3D cell cultures with defined parameters is challenging, with significant failure rates when embedding 3D multicellular spheroids into extracellular mimics. Here, we report a new 3D bioprinter we developed in conjunction with bioinks to allow 3D-multicellular spheroids to be produced in a high-throughput manner. Highthroughput production of embedded multicellular spheroids allowed entire drug-dose responses to be performed in 96-well plate format with statistically relevant numbers of data points. We have deconvoluted important parameters in drug responses including the impact of spheroid size and embedding in an extracellular matrix mimic on IC50 values.

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“…To build ARPE‐19 monolayer and acquire ARPE‐19 and Y79 cell seeding patterns utilizing microvalve‐based bioprinting are rarely reported. The microvalve‐based bioprinting technology can accurately create tissue models with a high degree of throughput (W. L. Ng, Lee, Yeong, & Win Naing, ). In this paper, a 3D retinal tissue model (Figure ) composed of ARPE‐19 and Y79 cells is bioprinted, and the bioprinted construct is meaningful for biomedical applications, for example, disease research and discovery of treatment options.…”

Section: Introductionmentioning

confidence: 99%

A bilayer photoreceptor-retinal tissue model with gradient cell density design: A study of microvalve-based bioprinting

Shi

Tan

Yeong

et al. 2018

J Tissue Eng Regen Med

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ARPE-19 and Y79 cells were precisely and effectively delivered to form an in vitro retinal tissue model via 3D cell bioprinting technology. The samples were characterized by cell viability assay, haematoxylin and eosin and immunofluorescent staining, scanning electrical microscopy and confocal microscopy, and so forth. The bioprinted ARPE-19 cells formed a high-quality cell monolayer in 14 days. Manually seeded ARPE-19 cells were poorly controlled during and after cell seeding, and they aggregated to form uneven cell layer. The Y79 cells were subsequently bioprinted on the ARPE-19 cell monolayer to form 2 distinctive patterns. The microvalve-based bioprinting is efficient and accurate to build the in vitro tissue models with the potential to provide similar pathological responses and mechanism to human diseases, to mimic the phenotypic endpoints that are comparable with clinical studies, and to provide a realistic prediction of clinical efficacy.

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Microvalve-based bioprinting – process, bio-inks and applications

Cited by 183 publications

References 116 publications

3D Bioprinting of Artificial Tissues: Construction of Biomimetic Microstructures

3D Bioprinting of Artificial Tissues: Construction of Biomimetic Microstructures

Precise, high-throughput production of multicellular spheroids with a bespoke 3D bioprinter

A bilayer photoreceptor-retinal tissue model with gradient cell density design: A study of microvalve-based bioprinting

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