Microtubules play a role in trafficking prevacuolar compartments to vacuoles in tobacco pollen tubes

Onelli, Elisabetta; Scali, Monica; Caccianiga, Marco; Stroppa, Nadia; Morandini, Piero; Pavesi, Giulio; Moscatelli, Alessandra

doi:10.1098/rsob.180078

Cited by 6 publications

(6 citation statements)

References 85 publications

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“…These findings challenge the assumed notion that, other than in secretion, microtubules have little involvement in vesicle and organelle movement in plants (28). In this challenge, our work joins other recent reports suggesting a role for microtubules in TGN biogenesis and tracking (74) and in trafficking between the MVB and the vacuole (75).…”

Section: Discussionsupporting

confidence: 78%

MTV proteins unveil ER- and microtubule-associated compartments in the plant vacuolar trafficking pathway

Delgadillo

Ruano

Zouhar

et al. 2020

Proc. Natl. Acad. Sci. U.S.A.

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The factors and mechanisms involved in vacuolar transport in plants, and in particular those directing vesicles to their target endomembrane compartment, remain largely unknown. To identify components of the vacuolar trafficking machinery, we searched for Arabidopsis modified transport to the vacuole (mtv) mutants that abnormally secrete the synthetic vacuolar cargo VAC2. We report here on the identification of 17 mtv mutations, corresponding to mutant alleles of MTV2/VSR4, MTV3/PTEN2A MTV7/EREL1, MTV8/ARFC1, MTV9/PUF2, MTV10/VPS3, MTV11/VPS15, MTV12/GRV2, MTV14/GFS10, MTV15/BET11, MTV16/VPS51, MTV17/VPS54, and MTV18/VSR1. Eight of the MTV proteins localize at the interface between the trans-Golgi network (TGN) and the multivesicular bodies (MVBs), supporting that the trafficking step between these compartments is essential for segregating vacuolar proteins from those destined for secretion. Importantly, the GARP tethering complex subunits MTV16/VPS51 and MTV17/VPS54 were found at endoplasmic reticulum (ER)- and microtubule-associated compartments (EMACs). Moreover, MTV16/VPS51 interacts with the motor domain of kinesins, suggesting that, in addition to tethering vesicles, the GARP complex may regulate the motors that transport them. Our findings unveil a previously uncharacterized compartment of the plant vacuolar trafficking pathway and support a role for microtubules and kinesins in GARP-dependent transport of soluble vacuolar cargo in plants.

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Section: Discussionsupporting

confidence: 78%

MTV proteins unveil ER- and microtubule-associated compartments in the plant vacuolar trafficking pathway

Delgadillo

Ruano

Zouhar

et al. 2020

Proc. Natl. Acad. Sci. U.S.A.

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“…The findings described in this section were mainly achieved using lily flower pollen, which has been widely used to study the growth kinetics of pollen tubes due to its rapid elongation property. A similar polarized distribution of vacuoles has been observed in other species, such as Arabidopsis and tobacco [ 16 , 42 , 43 ], implying that the homologous mechanism controlling vacuole dynamics might be conserved in angiosperms.…”

Section: Developmental Processessupporting

confidence: 73%

“…A previous study showed that the disruption of MTs by oryzalin did not cause any obvious defects in vacuolar status, suggesting that actin, rather than MTs, is primarily engaged in the maintenance of vacuolar morphology and distribution in pollen tubes [ 41 ]. However, a more recent report indicated that MTs are required for transporting prevacuolar compartments to large vacuoles in the shank of tobacco pollen tubes, which points to the possibility of MTs engagement in fine-tuning vacuolar dynamics [ 42 ].…”

Section: Developmental Processesmentioning

confidence: 99%

At the Nexus between Cytoskeleton and Vacuole: How Plant Cytoskeletons Govern the Dynamics of Large Vacuoles

Takatsuka

Higaki

Ito

2023

IJMS

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Large vacuoles are a predominant cell organelle throughout the plant body. They maximally account for over 90% of cell volume and generate turgor pressure that acts as a driving force of cell growth, which is essential for plant development. The plant vacuole also acts as a reservoir for sequestering waste products and apoptotic enzymes, thereby enabling plants to rapidly respond to fluctuating environments. Vacuoles undergo dynamic transformation through repeated enlargement, fusion, fragmentation, invagination, and constriction, eventually resulting in the typical 3-dimensional complex structure in each cell type. Previous studies have indicated that such dynamic transformations of plant vacuoles are governed by the plant cytoskeletons, which consist of F-actin and microtubules. However, the molecular mechanism of cytoskeleton-mediated vacuolar modifications remains largely unclear. Here we first review the behavior of cytoskeletons and vacuoles during plant development and in response to environmental stresses, and then introduce candidates that potentially play pivotal roles in the vacuole–cytoskeleton nexus. Finally, we discuss factors hampering the advances in this research field and their possible solutions using the currently available cutting-edge technologies.

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“…It has been shown that microtubules play an important role in abiotic stress due to changes in their location and structural organization and can cause changes in cell size [60][61][62]. Atypical components of the cytoskeleton in tomato root cells exposed to NaCl and Na 2 SO 4 at different levels of ROS, as well as shortening and thinning of microtubules, were observed in the cytoplasm of tobacco root cells in longitudinal and transverse sections [63].…”

Section: Discussionmentioning

confidence: 96%

Characteristics of Root Cells during In Vitro Rhizogenesis under Action of NaCl in Two Tomato Genotypes Differing in Salt Tolerance

Bogoutdinova

Баранова

Кононенко

et al. 2023

IJPB

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Understanding the mechanisms of plant salt tolerance as a complex trait is an integral part of many studies, the results of which have been used in the breeding process. The aim of this study was to compare the root response of two tomato (Solanum lycopersicum L.) genotypes (breeding line YaLF and cultivar Recordsmen) differing in salt tolerance. Rhizogenesis was induced in tomato shoots in vitro with different concentrations of NaCl in the culture medium. A number of morphobiological and cytological parameters were evaluated at the organ, tissue, and cellular levels for possible use in a comprehensive assessment of genotypes for salt tolerance. The influence of NaCl caused disruption of the cell cycle and redistribution of cells in the phases of the cell cycle. An increase in the degree of vacuolization was shown in cv Recordsmen at 75 and 150 mM NaCl and in the YaLF line at 150 mM NaCl. Under salt action, an increase/decrease in the length of cells such as columella cells (both genotypes) and epidermal cells (in cv Recordsmen at 75 and 150 mM NaCl) was shown. Differences between genotypes were demonstrated by changes in the area of the central cylinder and primary root cortex cells, as well as by changes of the Snucleolus/Snucleus ratio in these cells. Transmission electron microscopy (TEM) showed the modification of the chromatin structure in the root cells of these genotypes. Various cytoskeletal disorders were revealed in interphase cells of the tomato root of cv Recordsmen and the YaLF line by immunofluorescent staining under saline conditions. These morphometric and cytological parameters can be used for a comparative evaluation of genotypes differing in salt tolerance in a comprehensive assessment of varieties.

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Microtubules play a role in trafficking prevacuolar compartments to vacuoles in tobacco pollen tubes

Cited by 6 publications

References 85 publications

MTV proteins unveil ER- and microtubule-associated compartments in the plant vacuolar trafficking pathway

MTV proteins unveil ER- and microtubule-associated compartments in the plant vacuolar trafficking pathway

At the Nexus between Cytoskeleton and Vacuole: How Plant Cytoskeletons Govern the Dynamics of Large Vacuoles

Characteristics of Root Cells during In Vitro Rhizogenesis under Action of NaCl in Two Tomato Genotypes Differing in Salt Tolerance

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