Microtubules in<i>orbit</i>

Kline-Smith, Susan L.; Walczak, Claire

doi:10.1083/jcb.149.1.5

Cited by 4 publications

(3 citation statements)

References 11 publications

(11 reference statements)

Supporting

Mentioning

Contrasting

Order By: Relevance

“…Curved spindles are described in mitosis in mutant orbit in Drosophila (Inoue et al, 2000). The product of this gene has similarity to MAP4 and to STU1 proteins and co-localises with spindle MTs (Kline-Smith and Walczak, 2000).…”

Section: Early Prometaphasementioning

confidence: 99%

Formation of division spindles in higher plant meiosis

Shamina

2005

Cell Biology International

View full text Add to dashboard Cite

Depolymerisation of the MT cytoskeleton during late prophase makes it impossible to follow the cytoskeleton cycle in centrosomeless plant meiocytes. This paper describes rearrangements of the MT cytoskeleton during plant meiotic spindle formation in normally dividing pollen mother cells in various higher plant species and forms in which the cytoskeleton does not depolymerise at prophase. In such variants of the wild-type, cytoskeleton rearrangements can be observed at late prophase/early prometaphase. Radial MT bundles coalesce in the meridian plane, reorientate tangentially, curve and give rise to a developed ring-shaped perinuclear cytoskeleton system at the meridian. During nuclear envelope breakdown this ring disintegrates and splits into a set of free MT bundles. Three sub-stages of prometaphase are indicated: early prometaphase (disintegration of perinuclear ring and invasion of MTs into the former nuclear area), middle prometaphase or chaotic stage (formation of bipolar spindle fibres), and late prometaphase (formation of bipolar spindle). Analysis of a range of abnormal phenotypes (disintegrated, multiple, polyarchal, chaotic spindles) reveals two previously unknown processes during late prometaphase: axial orientation and consolidation of the spindle fibres.

show abstract

Section: Early Prometaphasementioning

confidence: 99%

Formation of division spindles in higher plant meiosis

Shamina

2005

Cell Biology International

View full text Add to dashboard Cite

show abstract

“…These proteins are the fly and yeast homologues of the CLASPs. Mitotic phenotypes of MAST/Orbit mutants were complex, but suggested that the protein was essential for spindle assembly and function, possibly by promoting microtubule stability (Kline-Smith and Walczak, 2000). Subsequent RNAi and time-lapse microscopy analysis of MAST alleles revealed that the protein is required for chromosome alignment and maintenance of spindle bipolarity in mitosis (Maiato et al, 2002).…”

Section: Introductionmentioning

confidence: 99%

Human CLASP1 Is an Outer Kinetochore Component that Regulates Spindle Microtubule Dynamics

Maiato

Fairley

Rieder

et al. 2003

Cell

202

243

View full text Add to dashboard Cite

One of the most intriguing aspects of mitosis is the ability of kinetochores to hold onto plus ends of microtubules that are actively gaining or losing tubulin subunits. Here, we show that CLASP1, a microtubule-associated protein, localizes preferentially near the plus ends of growing spindle microtubules and is also a component of a kinetochore region that we term the outer corona. A truncated form of CLASP1 lacking the kinetochore binding domain behaves as a dominant negative, leading to the formation of radial arrays of microtubule bundles that are highly resistant to depolymerization. Microinjection of CLASP1-specific antibodies suppresses microtubule dynamics at kinetochores and throughout the spindle, resulting in the formation of monopolar asters with chromosomes buried in the interior. Incubation with microtubule-stabilizing drugs rescues the kinetochore association with microtubule plus ends at the periphery of the asters. Our data suggest that CLASP1 is required at kinetochores for attached microtubules to exhibit normal dynamic behavior.

show abstract

“…Changes in the profiles of MT bundles during ontogenesis have been described in spiral cortical MTs (Lloyd et al, 1996). Mutations that change such profiles and lead to curved spindle formation are known in Drosophila melanogaster (Inoue et al, 2000;Kline-Smith and Walczak, 2000). Newly-synthesized polar MTs appear constantly to invade the phragmoplast fibers, replicating its profile, increasing their number and then participating in centrifugal movement.…”

Section: Discussionmentioning

confidence: 99%

Formation and function of phragmoplast during successive cytokinesis stages in higher plant meiosis

Shamina

Gordeeva

Kovaleva

et al. 2007

Cell Biology International

View full text Add to dashboard Cite

Cytoskeletal rearrangements were studied during meiotic telophase in a number of monocotyledonous plant species. Wild type and abnormal meiosis (in wide cereal hybrids, meiotic mutants and allolines) was analyzed. It was found that central spindle fibers that move centrifugally, along with newly-formed MTs, are the basis of phragmoplast formation and function in PMCs of monocotyledonous plant species with successive cytokinesis stages. A model for centrifugal movement of the meiotic phragmoplast is proposed; this model is a modification of the corresponding process during B-anaphase.

show abstract

Microtubules inorbit

Cited by 4 publications

References 11 publications

Formation of division spindles in higher plant meiosis

Formation of division spindles in higher plant meiosis

Human CLASP1 Is an Outer Kinetochore Component that Regulates Spindle Microtubule Dynamics

Formation and function of phragmoplast during successive cytokinesis stages in higher plant meiosis

Contact Info

Product

Resources

About