Microtubule organization in cultured soybean and black spruce cells: Interphase ?mitosis transition and spindle morphology

Wang, H.; Cutler, Adrian J.; Fowke, Larry C.

doi:10.1007/bf01403900

Cited by 19 publications

(5 citation statements)

References 30 publications

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“…The present data favor the hypothesis that the prophase and the metaphase spindle of floweringplant cells are two different and independent Mt arrays and that the latter is formed de novo at prometaphase (see also Kubiak et al 1986, Wang et al 1991a. This view is supported by the observation of atypical metaphase spindles, consisting of k-Mt bundles, in all the EB-affected metaphase cells (Fig.…”

Section: Metaphase Spindle Organizationsupporting

confidence: 88%

Study of mitosis in root-tip cells ofTriticum turgidum treated with the DNA-intercalating agent ethidium bromide

2000

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This work examines mitosis in root-tip cells of Triticum turgidurn treated with the RNA synthesis inhibitor ethidium bromide, using tubulin immunolabeling and electron microscopy. The following aberrations were observed in ethidium bromideaffected cells: (1) incomplete chromatin condensation and nuclearenvelope breakdown; (2) delay of preprophase microtubule band maturation; (3) preprophase microtubule band assembly in cells displaying an interphase appearance of the nucleus; (4) prevention of the prophase spindle formation, caused by inhibition of perinuclear microtubule (Mt) formation and/or inability of the perinuclear Mts to assume bipolarity; (5) organization of an atypical metaphase spindle which is unable to arrange the chromosomes on the equatorial plane; (6) formation of an atypical "perinuclear metaphase spindle" in cells in which nuclear-envelope breakdown has been almost completely inhibited; (7) inhibition of the anaphase spindle formation as well as of anaphase chromosome movement; (8) disorganization of the atypical mitotic spindle during transition from mitosis to cytokinesis. The observations favor the following hypotheses. Nucleation of prophase spindle Mts is related to the mechanism that causes nuclear-envelope breakdown. The mitotic poles lack Mtnucleating and -organizing properties, and their function does not account for prophase and metaphase spindle assembly. The organization of the prophase spindle is not a prerequisite for the formation of the metaphase spindle; the metaphase spindle seems to be formed de novo by Mts nucleated on the nuclear envelope and/or in the immediate vicinity of chromosomes.

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Section: Metaphase Spindle Organizationsupporting

confidence: 88%

Study of mitosis in root-tip cells ofTriticum turgidum treated with the DNA-intercalating agent ethidium bromide

2000

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“…The metaphase MT-spindle is barrel-shaped with broad spindle poles and consists primarily of kinetochore-MT (K-MT) bundles ( Fig. 1I) as described in other gymnosperms (Fowke, 1993;Gilmer et al, 1999;Wang et al, 1991). AFs were not observed in the interior of the metaphase spindle ( Fig.…”

Section: Prometaphase/metaphase Cellsmentioning

confidence: 72%

Organization of the endoplasmic reticulum in dividing cells of the gymnosperms Pinus brutia and Pinus nigra, and of the pterophyte Asplenium nidus

Zachariadis

Quader

Galatis

et al. 2003

Cell Biology International

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Endoplasmic reticulum (ER) organization in the dividing cells of the pterophyte Asplenium nidus and of the gymnosperms Pinus brutia and Pinus nigra has been studied by immunolocalization techniques using the monoclonal antibody 2E7, which recognizes luminar ER resident proteins containing C-terminal HDEL sequences. In the pterophyte, the ER reorganization during cell cycle is similar to that in angiosperms. Among others, prominent ER gatherings were found at the mitotic spindle poles and in the phragmoplast during cytokinesis. However, in the gymnosperms examined, the ER displays a unique pattern of reorganization not described so far. In both the Pinus species, well-defined ER patterns are successively formed during cell cycle. They are the preprophase ER-band, the prophase- metaphase- and anaphase ER-spindle, the interzonal ER-system, the ER-phragmoplast and an ER-system lining the daughter cell wall. The ER patterns are closely similar to that of the correspondent microtubule (MT) arrangements with which they are co-organized. Observations made on P. nigra root-cells affected by oryzalin, colchicine and cytochalasin D favour the conclusion that the pattern of ER organization is controlled during mitosis and cytokinesis by the MT cytoskeleton.

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“…According to our data, this process is realised by a special mechanism that can be aberrated, and it consists of at least two stages: disintegration of the ring into separate cytoskeletal elements and their disorientation. At mitosis such ''chaotisation'' is described in the transfer from prophase spindle to prometaphase chaotic figure, the bipolar prometaphase spindle with convergent poles disintegrates into separate fibres which become chaotically orientated (DeMey et al, 1982;Wang et al, 1991). An interesting abnormality of cytoskeleton cycle that can be an illustration of aberration in the chaotisation process is described in the meiosis of Paspalum: developed metaphase spindles suddenly disintegrate and transform into a chaotic fibre net returning to the configuration of midprometaphase (Pagliarini et al, 1998).…”

Section: Early Prometaphasementioning

confidence: 99%

Formation of division spindles in higher plant meiosis

Shamina

2005

Cell Biology International

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Depolymerisation of the MT cytoskeleton during late prophase makes it impossible to follow the cytoskeleton cycle in centrosomeless plant meiocytes. This paper describes rearrangements of the MT cytoskeleton during plant meiotic spindle formation in normally dividing pollen mother cells in various higher plant species and forms in which the cytoskeleton does not depolymerise at prophase. In such variants of the wild-type, cytoskeleton rearrangements can be observed at late prophase/early prometaphase. Radial MT bundles coalesce in the meridian plane, reorientate tangentially, curve and give rise to a developed ring-shaped perinuclear cytoskeleton system at the meridian. During nuclear envelope breakdown this ring disintegrates and splits into a set of free MT bundles. Three sub-stages of prometaphase are indicated: early prometaphase (disintegration of perinuclear ring and invasion of MTs into the former nuclear area), middle prometaphase or chaotic stage (formation of bipolar spindle fibres), and late prometaphase (formation of bipolar spindle). Analysis of a range of abnormal phenotypes (disintegrated, multiple, polyarchal, chaotic spindles) reveals two previously unknown processes during late prometaphase: axial orientation and consolidation of the spindle fibres.

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Microtubule organization in cultured soybean and black spruce cells: Interphase ?mitosis transition and spindle morphology

Cited by 19 publications

References 30 publications

Study of mitosis in root-tip cells ofTriticum turgidum treated with the DNA-intercalating agent ethidium bromide

Study of mitosis in root-tip cells ofTriticum turgidum treated with the DNA-intercalating agent ethidium bromide

Organization of the endoplasmic reticulum in dividing cells of the gymnosperms Pinus brutia and Pinus nigra, and of the pterophyte Asplenium nidus

Formation of division spindles in higher plant meiosis

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