Microtubule organization and cell division in embryogenie protoplast cultures of white spruce (Picea glauca)

Fowke, Larry C.; Attree, S. M.; Wang, H.; Dunstan, David I.

doi:10.1007/bf01323277

Cited by 30 publications

(9 citation statements)

References 18 publications

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“…Conifer somatic embryos are an ideal source of totipotent protoplasts, and a number of reports have described somatic embryogenesis and plant regeneration from isolated protoplasts (Table 2). Egertsdotter &von Arnold 1993 Attree et al 1987, 1989aBekkaoui et al 1987Attree et al 1989bFowke et al 1990Tautorus et al 1990 Gupta & Durzan 1987bGupta et al 1988 Lain~ & David 1990 Gupta et al 1988 *Embryogenic tissue derived from haploid megagametophytes…”

Section: Somatic Embryogenesismentioning

confidence: 99%

Embryogeny of gymnosperms: advances in synthetic seed technology of conifers

Attree

Fowke

1993

Plant Cell Tiss Organ Cult

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295

130

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Synthetic seed technology requires the inexpensive production of large numbers of high-quality somatic embryos. Proliferating embryogenic cultures from conifers consist of immature embryos, which undergo synchronous maturation in the presence of abscisic acid and elevated osmoticum. Improvements in conifer somatic embryo quality have been achieved by identifying the conditions in vitro that resemble the conditions during in ovulo development of zygotic embryos. One normal aspect of zygotic embryo development for conifers is maturation drying, which allows seeds to be stored and promotes normal germination. Conditions of culture are described that yield mature conifer somatic embryos that possess normal storage proteins and fatty acids and which survive either partial drying, or full drying to moisture contents similar to those achieved by mature dehydrated zygotic embryos. Large numbers of quiescent somatic embryos can be produced throughout the year and stored for germination in the spring, which simplifies production and provides plants of uniform size. This review focuses on recent advances in conifer somatic embryogenesis and synthetic seed technology, particularly in areas of embryo development, maturation drying, encapsulation and germination. Comparisons of conifer embryogeny are made with other gymnosperms and angiosperms.Abbreviations: ABA-abscisic acid, LEA-late embryogenesis abundant, PEG-polyethylene glycol, PGR-plant growth regulator, RH-relative humidity, TAG-triacylglycerol

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Section: Somatic Embryogenesismentioning

confidence: 99%

Embryogeny of gymnosperms: advances in synthetic seed technology of conifers

Attree

Fowke

1993

Plant Cell Tiss Organ Cult

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295

130

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“…DAPI at a concentration of 1 ~tg ml-1 in microtubule stabilizing buffer [MtSB : 100 mM PIPES (pH 6.9), 10 mM EGTA, 10 mM MgSO4] containing 9% mannitol was used to stain nuclear DNA (Fowke et al 1990). All observations were made with a Nikon optiphot epi-flourescence microscope using appropriate filter sets (excitation 330-380 nm, Barrier 420 nm).…”

Section: Nuclear Staining With Dapl(46-diamidino-2-phenylindole)mentioning

confidence: 99%

Plant regeneration from petal protoplast culture ofPetunia hybrida

Kim

1994

Plant Cell Tiss Organ Cult

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Morphologically normal plants have been regenerated from petal protoplasts of petunia (Petunia hybrida) flower. Maximum protoplast yields from petal tissues were obtained within 2 days after anthesis. Protoplasts were cultured on modified Murashige and Skoog's medium in which NH4NO3 and Fe.EDTA concentrations were reduced to 1/3 (7mM) and 1/10 (10 ~tM), respectively. After plating, protoplasts gradually reduced pigment density, and plastids developed near the nucleus. In premitotic petunia petal cells, the nucleus moved from the periphery to the central region of the cell. The first cell divisions were detected after 6-10 days of culture initiation, and the average division frequency was 15% in the best culture condition. The results indicated that the time of the first cell division and cell division frequency were closely related to flower age after anthesis. More than a hundred plants with morphologically normal shoots and roots have been obtained. Those plants grew vigorously in soil.

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“…Randomly oriented cortical-MT arrays were observed in protoplasts derived from cellsuspension cultures of soybean (Wang et al 1989a) and maize (Wang et al 1989d), and from embryogenic cultures of white spruce (Fowke et al 1990) and larch (Staxen et al 1990). Although the cortical-MT arrangements in cells from the above cultures have not been reported, others have attempted to correlate the cortical-MT organization of protoplasts and the cells from which they were derived.…”

Section: Cortical Microtubule Organizationmentioning

confidence: 99%

Microtubules in cultured plant protoplasts

Simmonds¹

1991

Acta Botanica Neerlandica

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Microtubule organization and cell division in embryogenie protoplast cultures of white spruce (Picea glauca)

Cited by 30 publications

References 18 publications

Embryogeny of gymnosperms: advances in synthetic seed technology of conifers

Embryogeny of gymnosperms: advances in synthetic seed technology of conifers

Plant regeneration from petal protoplast culture ofPetunia hybrida

Microtubules in cultured plant protoplasts

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