1990
DOI: 10.1007/bf01323277
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Microtubule organization and cell division in embryogenie protoplast cultures of white spruce (Picea glauca)

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Cited by 30 publications
(9 citation statements)
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“…Conifer somatic embryos are an ideal source of totipotent protoplasts, and a number of reports have described somatic embryogenesis and plant regeneration from isolated protoplasts (Table 2). Egertsdotter &von Arnold 1993 Attree et al 1987, 1989aBekkaoui et al 1987Attree et al 1989bFowke et al 1990Tautorus et al 1990 Gupta & Durzan 1987bGupta et al 1988 Lain~ & David 1990 Gupta et al 1988 *Embryogenic tissue derived from haploid megagametophytes…”
Section: Somatic Embryogenesismentioning
confidence: 99%
“…Conifer somatic embryos are an ideal source of totipotent protoplasts, and a number of reports have described somatic embryogenesis and plant regeneration from isolated protoplasts (Table 2). Egertsdotter &von Arnold 1993 Attree et al 1987, 1989aBekkaoui et al 1987Attree et al 1989bFowke et al 1990Tautorus et al 1990 Gupta & Durzan 1987bGupta et al 1988 Lain~ & David 1990 Gupta et al 1988 *Embryogenic tissue derived from haploid megagametophytes…”
Section: Somatic Embryogenesismentioning
confidence: 99%
“…DAPI at a concentration of 1 ~tg ml-1 in microtubule stabilizing buffer [MtSB : 100 mM PIPES (pH 6.9), 10 mM EGTA, 10 mM MgSO4] containing 9% mannitol was used to stain nuclear DNA (Fowke et al 1990). All observations were made with a Nikon optiphot epi-flourescence microscope using appropriate filter sets (excitation 330-380 nm, Barrier 420 nm).…”
Section: Nuclear Staining With Dapl(46-diamidino-2-phenylindole)mentioning
confidence: 99%
“…Randomly oriented cortical-MT arrays were observed in protoplasts derived from cellsuspension cultures of soybean (Wang et al 1989a) and maize (Wang et al 1989d), and from embryogenic cultures of white spruce (Fowke et al 1990) and larch (Staxen et al 1990). Although the cortical-MT arrangements in cells from the above cultures have not been reported, others have attempted to correlate the cortical-MT organization of protoplasts and the cells from which they were derived.…”
Section: Cortical Microtubule Organizationmentioning
confidence: 99%