Microtubule-dependent transport and dynamics of vimentin intermediate filaments

Hookway, Caroline; Ding, Liya; Davidson, Michael W.; Rappoport, Joshua Z.; Danuser, Gaudenz; Gelfand, Vladimir I.

doi:10.1091/mbc.e14-09-1398

Cited by 87 publications

(136 citation statements)

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“…In agreement with our previously published results, we observed the presence of some short and uniformly red segments of filaments that have been transported along microtubules outside the photoconverted zone ( Fig. 4A) (16). However, no dual-color filaments were observed outside the photoconverted area in these cells, even 15 min after photoconversion (Fig.…”

Section: Y117l Vimentin Does Not Exchange In the Context Of Mature VIsupporting

confidence: 93%

“…Previous studies have shown that VIFs are very stable because the exchange of fragments or smaller subunits along the length of mature filaments is very slow (16,22). The present study revealed a very fast process of subunit exchange among ULFs assembled from vimentin Y117L .…”

Section: Y117l Vimentin Does Not Exchange In the Context Of Mature VIsupporting

confidence: 58%

“…Recent studies using photoactivation and photoconversion demonstrate that reorganization of VIFs and neurofilaments mainly occur via a different mechanism. Instead of releasing soluble subunits, these filaments reorganize through a process of severing and end-to-end reannealing (15,16). In general, assembly and disassembly of IFs is believed to be regulated by phosphorylation or other posttranslational modifications (see ref.…”

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confidence: 99%

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Vimentin filament precursors exchange subunits in an ATP-dependent manner

Ahrends

Rossow

Hookway

et al. 2015

Proc. Natl. Acad. Sci. U.S.A.

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Intermediate filaments (IFs) are a component of the cytoskeleton capable of profound reorganization in response to specific physiological situations, such as differentiation, cell division, and motility. Various mechanisms were proposed to be responsible for this plasticity depending on the type of IF polymer and the biological context. For example, recent studies suggest that mature vimentin IFs (VIFs) undergo rearrangement by severing and reannealing, but direct subunit exchange within the filament plays little role in filament dynamics at steady state. Here, we studied the dynamics of subunit exchange in VIF precursors, called unit-length filaments (ULFs), formed by the lateral association of eight vimentin tetramers. To block vimentin assembly at the ULF stage, we used the Y117L vimentin mutant (vimentin Y117L ). By tagging vimentin Y117L with a photoconvertible protein mEos3.2 and photoconverting ULFs in a limited area of the cytoplasm, we found that ULFs, unlike mature filaments, were highly dynamic. Subunit exchange among ULFs occurred within seconds and was limited by the diffusion of soluble subunits in the cytoplasm rather than by the association and dissociation of subunits from ULFs. Our data demonstrate that cells expressing vimentin Y117L contained a large pool of soluble vimentin tetramers that was in rapid equilibrium with ULFs. Furthermore, vimentin exchange in ULFs required ATP, and ATP depletion caused a dramatic reduction of the soluble tetramer pool. We believe that the dynamic exchange of subunits plays a role in the regulation of ULF assembly and the maintenance of a soluble vimentin pool during the reorganization of filament networks.ell shape, mechanical properties, and motile behavior are determined by the cytoskeleton composed of three interconnected polymers: actin microfilaments, microtubules, and intermediates filaments (IFs). Whereas the structural polarity of microfilaments and microtubules is essential for their functions, IFs are apolar structures that contribute to the mechanical properties of the cell (reviewed in ref.

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Section: Y117l Vimentin Does Not Exchange In the Context Of Mature VIsupporting

confidence: 93%

Section: Y117l Vimentin Does Not Exchange In the Context Of Mature VIsupporting

confidence: 58%

mentioning

confidence: 99%

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Vimentin filament precursors exchange subunits in an ATP-dependent manner

Ahrends

Rossow

Hookway

et al. 2015

Proc. Natl. Acad. Sci. U.S.A.

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“…In our case, we studied: the relationship between the sizes and lifetimes of clathrin-coated pits (CCPs), the role of actin in clathrin-mediated endocytosis, the dynamics at clathrin patches and hot spots of CCP formation, the formation of nanoscale actin rings, the diversity of caveolin-rich structures, and the nanoscale recruitment of a-actinin during the formation of actin-rich structures. Elsewhere, in the past year or two live-cell SIM has been used to investigate the dynamics of MreB filaments in bacteria (9) and cortical microtubules (10), microtubule-dependent transport of vimentin filaments (11), vesicle formation at the inner nuclear membrane (12), centrosome assembly in Drosophila (13), a dorsal actomyosin network that drives cell motility (14), and the assembly of isoforms of nonmuscle myosin II (15) and combinations of myosin II and myosin 18 (16) into bipolar filaments. We look forward to revisiting our debate with Sahl et al a decade from now to see which SR methods have had the broadest effect in biology.…”

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confidence: 99%

Response to Comment on “Extended-resolution structured illumination imaging of endocytic and cytoskeletal dynamics”

Dong

Betzig

2016

Science

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“…The presence of vimentin and crystalline alpha proteins increases stability of the cytoskeleton by reducing depolymerization. 56,57 The results of decreased depolymerization is an increase of the cytoskeleton with membrane protrusion, tumor progression and metastasis, as well as a change of polarization during mitosis leading to cellular pleomorphism. 58,59 The presence of HSP 60 in the primary tumor indicates the refurbishment of misfolding proteins and affects the stability of the cytoskeleton, thus enhancing the progression of carcinogenesis.…”

Section: Resultsmentioning

confidence: 99%

Morpho/Proteomic Comparative between High Grade Pleomorphic Sarcoma and Metastasis Diagnosed in an Old Captive Common Hippo

Silva¹,

Lacerda²,

Faria³

et al. 2017

J. Braz. Chem. Soc.

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Old age is a risk factor for cancer development in humans and animals, and studies have shown that tumors in animals are acceptable models for studying human cancers, considering the similarities between their factors. This work was conducted in a 53-year-old captive female common hippo (Hippopotamus amphibious) with a left leg tumor and metastatic mass. Histopathological and immunohistochemical analyses were carried out with a final diagnosis of a high grade pleomorphic sarcoma. A proteomic study using mass spectrometry was added in order to identify further aspects of the primary tumor and metastasis which could improve our understanding, and each tissue showed a proteomic profile indicative of its pathologic state with significant differences between healthy tissue, primary and metastatic tumors. Low levels of β-actin in primary tumors were identified, and this may be associated with a possible consequence of cytoskeleton dynamic modification. In metastatic tissue, these dynamics may be affected by the presence of HSP chaperone 60. Keywords:Hippopotamus amphibious, high grade pleomorphic sarcoma, cytoskeleton, β-actin, mass spectrometry IntroductionOncogenic phenomena in animals include a wide variety of benign and malignant tumors that develop during the life of the animal and have several health consequences. 1 Species with large bodies tend to have a lower cancer rate, which seems to indicate the presence of complex tumor detection systems (Peto's paradox).2 To illustrate this paradox, in contrast with humans, who have only two copies of the TP53 tumor suppressor gene, elephants, the largest of the land mammmals, have 20 copies of the TP53 gene. This allows for extra efficiency in their systems, enabling the correction of DNA damage or inducing apoptosis in mutated cells. As a result, only 3% of elephants develop cancer. 3,4 Despite some comparative cancer and lifespan analyses between wild and zoo animals showing that captive animals were susceptible to cancer and decreased longevity. 5,6 Tidière et al. 7 revealed that mammals from zoo populations generally lived longer than their wild counterparts (84% of species). Of particular interest, the common hippopotamus (Hippopotamus amphibius), a robust mammal with an estimated 40-year lifespan in nature and with slow pace of life, has increased longevity in captivity. [7][8][9] The relationship between life expectancy and environment may be associated with the low caloric diet and reduced metabolism present in captive animals, which slows ageing and the appearance of cancer cases. This is also true for humans. 10,11 Paradoxically, the exceptional longevity these captive animals exhibit can be associated with cancer due to several factors such as telomere shortening, increased cellular senescence, accumulation mutation of DNA in stem and progenitor cells required for active replenishment during the lifespan of the organism, mutations in tumor suppressor genes and metabolic changes. 12-14Based on available database information and proteomics approach, it is poss...

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Microtubule-dependent transport and dynamics of vimentin intermediate filaments

Cited by 87 publications

References 43 publications

Vimentin filament precursors exchange subunits in an ATP-dependent manner

Vimentin filament precursors exchange subunits in an ATP-dependent manner

Response to Comment on “Extended-resolution structured illumination imaging of endocytic and cytoskeletal dynamics”

Morpho/Proteomic Comparative between High Grade Pleomorphic Sarcoma and Metastasis Diagnosed in an Old Captive Common Hippo

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