Microtubule bundle formation driven by ATP: the effect of concentrations of kinesin, streptavidin and microtubules

Kawamura, Ryuzo; Kakugo, Akira; Osada, Yoshihito; Gong, Jian Ping

doi:10.1088/0957-4484/21/14/145603

Cited by 33 publications

(39 citation statements)

References 24 publications

(34 reference statements)

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“…Our average loop radius is about 1 µm, whereas spools are typically larger 2-10 µm in radius (Hess et al 2005, Kawamura et al 2008, Liu et al 2008. Fourth, previous groups have found that spools form and rotate in a counter-clockwise direction (Kawamura et al 2010a). We find there is no preference for a specific rotation direction of our loops (supplemental figure 1 available at stacks.iop.…”

Section: Resultsmentioning

confidence: 49%

“…Previous studies have observed the formation of microtubule 'spools' created using microtubule-gliding assays and cross-linked microtubules that bundle together (Liu et al 2008, Hess et al 2005, Kawamura et al 2008, 2010b, 2010a. The likely mechanism of spool formation appears to be pinning of a bundle on a dead kinesin in the gliding chamber that allows for high curvature and then cross-linking of the bundle to itself (Hess et al 2005).…”

Section: Resultsmentioning

confidence: 99%

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Loop formation of microtubules during gliding at high density

Liu¹,

Tüzel²,

Ross³

2011

J. Phys.: Condens. Matter

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The microtubule cytoskeleton, including the associated proteins, forms a complex network essential to multiple cellular processes. Microtubule-associated motor proteins, such as kinesin-1, travel on microtubules to transport membrane bound vesicles across the crowded cell. Other motors, such as cytoplasmic dynein and kinesin-5, are used to organize the cytoskeleton during mitosis. In order to understand the self-organization processes of motors on microtubules, we performed filament-gliding assays with kinesin-1 motors bound to the cover glass with a high density of microtubules on the surface. To observe microtubule organization, 3% of the microtubules were fluorescently labeled to serve as tracers. We find that microtubules in these assays are not confined to two dimensions and can cross one other. This causes microtubules to align locally with a relatively short correlation length. At high density, this local alignment is enough to create 'intersections' of perpendicularly oriented groups of microtubules. These intersections create vortices that cause microtubules to form loops. We characterize the radius of curvature and time duration of the loops. These different behaviors give insight into how crowded conditions, such as those in the cell, might affect motor behavior and cytoskeleton organization.

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Section: Resultsmentioning

confidence: 49%

Section: Resultsmentioning

confidence: 99%

Loop formation of microtubules during gliding at high density

Liu¹,

Tüzel²,

Ross³

2011

J. Phys.: Condens. Matter

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“…MT concentration was fixed at 1000 nM and the crosslinker ratio (St/Bt) was kept at 1/3.8 for allowing MTs to preferentially form ring-shaped assembly by AcSO. 10,11 Fig.…”

Section: Resultsmentioning

confidence: 99%

“…8,9 Depending on the experimental conditions such as MT concentration and crosslinker ratio (St/Bt), AcSO of MTs provides bundle-, network-, and ring-shaped assemblies which generate translational, amoeboid and rotational motion respectively. 10,11 Among them, the ring-shaped assembly have been attracting considerable attention and appears to be a promising candidate for application in nanotechnology as this non-equilibrium structure is capable of storing a huge amount of bending energy 8 and can provide continuous work without changing the position of the mass center. [12][13][14][15][16] Therefore, it is important to understand how different factors affect the formation and properties of ring-shaped MT assemblies such as rotational direction, thickness (subtraction of inner diameter from outer diameter), size etc..…”

Section: Introductionmentioning

confidence: 99%

Effect of length and rigidity of microtubules on the size of ring-shaped assemblies obtained through active self-organization

et al. 2015

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The microtubule (MT)-kinesin biomolecular motor system has attracted much attention due to its possible applications in artificial biomachines. Recently an active self-organization (AcSO) method has been established to integrate MT filaments into highly organized assembled structures. The ring-shaped MT assembly, one of the structures derived from the AcSO of MTs, can convert the translational motion of MTs into rotational motion. Due to this attractive feature, the ring-shaped MT assembly appears to be a promising candidate for developing artificial devices and for future nanotechnological applications. In this work, we have investigated the effect of length and rigidity of MT filaments on the size of ring-shaped MT assembly in the AcSO process. We show that the size of ring-shaped MT assembly can be controlled by tuning the length and rigidity of MT filaments employed in the AcSO.Longer and stiffer MT filaments led to larger ring-shaped assemblies through AcSO, while AcSO of shorter and less stiff MT filaments produced smaller ring-shaped assemblies. This work might be important for the development of biomolecular motor based artificial biomachines, especially where size control of ring-shaped MT assembly will play an important role.

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“…(Fig. 34.2b) [21,22]. Bundles, networks, and ring-shaped microtubule assemblies offer diverse functions, quite different from that of a single microtubule filament, as reflected through translational, amoeboid and rotational motion, respectively ( Fig.…”

Section: Active Self-organization Of Biomolecular Motorsmentioning

confidence: 99%

ATP-Driven Bio-machine

et al. 2014

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Biomolecular motor systems microtubule/kinesin, actin/myosin are constituent components of biological power unit, which can perform mechanical work by converting chemical energy obtained from hydrolysis of adenosine triphosphate (ATP). The biomolecular motors are organized into highly complex and ordered structures as observed in muscle, stress fiber, contractile ring which exhibit various functions. Performance of these natural machines are much attractive compared to man-made machine. Therefore, to utilize the advantage of these natural machines biological motors have been proposed as the building blocks of ATP-driven bio-machine. In this chapter, different methodologies for designing biomolecular motor based bio-machines through non-equilibrium self-organization process are described.

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Microtubule bundle formation driven by ATP: the effect of concentrations of kinesin, streptavidin and microtubules

Cited by 33 publications

References 24 publications

Loop formation of microtubules during gliding at high density

Loop formation of microtubules during gliding at high density

Effect of length and rigidity of microtubules on the size of ring-shaped assemblies obtained through active self-organization

ATP-Driven Bio-machine

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