Doublecortin (DCX) is a neuronal microtubule-associated protein (MAP) that binds directly to microtubules via two Doublecortin (DC) domains. The DC domains sense the nucleotide state, longitudinal curvature, and protofilament number of the microtubule lattice, indicating a role in the regulation of microtubule structure in neurons. Mutations in DCX cause lissencephaly and subcortical band heterotopia (also known as double-cortex syndrome) due to impaired neuronal migration. To better understand the role of DCX in neuronal migration, we developed a model system based on induced pluripotent stem cells (iPSCs). We used CRISPR/Cas9 to knock out the Dcx gene in iPSCs and differentiated the cells into cortical neurons. Compared to control neurons, the DCX-KO neurons showed reduced velocities of nuclear movements, consistent with a neuronal migration phenotype. The reduced velocities correlated with an increase in the number of branches early in the neuronal development process, coherent with previous findings in a DCX-KO mouse model. Neurite branching is regulated by a host of microtubule-associated proteins (MAPs) and other protein factors, as well as by microtubule polymerization dynamics. However, microtubule dynamics were unchanged in DCX-KO neurons, with similar growth rates, lifetimes, and numbers. Rather, microtubule post-translational modifications were altered in DCX-KO neurons, where we observe a significant reduction in polyglutamylation. Polyglutamylation is usually abundant in neurons and regulates microtubule severing enzymes and intracellular trafficking by molecular motors. Consistently, we observe that lysosomes in DCX-KO neurons show a reduction of their processivity. We propose that the reduction of polyglutamylation leads to increased neurite branching and thus reduced neuronal migration. Our results indicate an unexpected role for DCX in the homeostasis of the tubulin code.
