Microtubule-associated protein 2 phosphorylation is decreased in the human epileptic temporal lobe cortex

Sánchez, Carlos Manuel Fernández; Arellano, Jon I.; Rodríguez-Sánchez, P; Ávila, Jesús; DeFelipe, Javier; Díez‐Guerra, F. Javier

doi:10.1016/s0306-4522(01)00338-4

Cited by 15 publications

(16 citation statements)

References 65 publications

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“…Western blots showed a single band of immunoreactive product, whose intensity was proportional to the amount of antigen. The staining pattern was identical to published data for human (Sanchez et al, 2001).…”

Section: Methodssupporting

confidence: 88%

Differential distribution of neurons in the gyral white matter of the human cerebral cortex

García-Marín

Blázquez-Llorca

Toledo-Rodriguez

et al. 2010

J of Comparative Neurology

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The neurons in the cortical white matter (WM neurons) originate from the first set of postmitotic neurons that migrates from the ventricular zone. In particular, they arise in the subplate that contains the earliest cells generated in the telencephalon, prior to the appearance of neurons in gray matter cortical layers. These cortical WM neurons are very numerous during development, when they are thought to participate in transient synaptic networks, although many of these cells later die, and relatively few cells survive as WM neurons in the adult. We used light and electron microscopy to analyze the distribution and density of WM neurons in various areas of the adult human cerebral cortex. Furthermore, we examined the perisomatic innervation of these neurons and estimated the density of synapses in the white matter. Finally, we examined the distribution and neurochemical nature of interneurons that putatively innervate the somata of WM neurons. From the data obtained, we can draw three main conclusions: first, the density of WM neurons varies depending on the cortical areas; second, calretinin-immunoreactive neurons represent the major subpopulation of GABAergic WM neurons; and, third, the somata of WM neurons are surrounded by both glutamatergic and GABAergic axon terminals, although only symmetric axosomatic synapses were found. By contrast, both symmetric and asymmetric axodendritic synapses were observed in the neuropil. We discuss the possible functional implications of these findings in terms of cortical circuits.

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Section: Methodssupporting

confidence: 88%

Differential distribution of neurons in the gyral white matter of the human cerebral cortex

García-Marín

Blázquez-Llorca

Toledo-Rodriguez

et al. 2010

J of Comparative Neurology

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“…Their significant increase in response to RA treatment may promote the expression of neuronal markers. Previous studies have shown that RA up-regulates the expression of neuronal markers, confirming that RA affects the differentiation of SK-N-SH cells [Lombet et al, 2001;Sánchez et al, 2001;Sarkanen et al, 2007].…”

Section: Discussionsupporting

confidence: 63%

Differential expression of nuclear matrix proteins during the differentiation of human neuroblastoma SK‐N‐SH cells induced by retinoic acid

Liang¹,

Li²,

Zhang³

et al. 2009

J of Cellular Biochemistry

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To investigate the alteration of nuclear matrix proteins (NMPs) during the differentiation of neuroblastoma SK-N-SH cells induced by retinoic acid (RA), differentiation markers were detected by immunocytochemistry and NMPs were selectively extracted and subjected to two-dimensional gel electrophoresis analysis. Immunocytochemical observation demonstrated that the expression of neuronal markers was up-regulated in SK-N-SH cells following RA treatment. Meanwhile, 52 NMPs (41 of which were identified) changed significantly during SK-N-SH differentiation; four of these NMPs were further confirmed by immunoblotting. This study suggests that the differentiation of neuroblastoma cells was accompanied by the altered expression of neuronal markers and NMPs. The presence of some differentially expressed NMPs was related to the proliferation and differentiation of neuroblastomas. Our results may help to reveal the relationship between NMPs and neuroblastoma carcinogenesis and reversion, as well as elucidate the regulatory principals driving neural cell proliferation and differentiation.

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“…Equivalent tissue samples subjected to Western blot analysis, however, did not show the same variations in MAP 1B concentration. Discrepancies between these two techniques have been previously reported by other groups (Ramon-Cueto and Avila 1999; Sanchez et al 2001). The differences might be methodological and possibly related to the finding that protein expression that occurs in specific areas in the same tissue is below the detection levels on Western blot because of the dilution of proteins in extracts prepared from a large mass of tissue.…”

Section: Discussionmentioning

confidence: 58%

Cells positive for microtubule-associated protein 1B (MAP 1B) are present along rat and human efferent ductules and epididymis

et al. 2006

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Microtubule-associated protein 1B (MAP 1B) is a neuronal cytoskeleton marker with predominant expression in the developing nervous system. The present study provides evidence for the expression of this cytoskeleton protein in non-neuronal and neuronal cells along rat and human efferent ductules and epididymis (initial segment, caput, and cauda). Reverse transcription/polymerase chain reaction and Western blot analysis were used to confirm the presence of MAP 1B (mRNA and protein) in rat tissues. Immunohistochemical studies revealed MAP-1B-positive staining in columnar ciliated cells present in efferent ductules and in narrow cells located in the initial segment, in both rat and human. MAP-1B-positive basal cells, located underneath the columnar cells, were only identified in the initial segment and caput epididymidis of the rat. Qualitative analysis of tissues from 40-day-old and 120-day-old rats indicated that the number of MAP-1B-positive ciliated, narrow, and basal cells per tubule increased with sexual maturation. These immunoreactive cells did not stain for dopamine beta-hydroxylase or acetylcholinesterase, indicating that they were not adrenergic or cholinergic in nature. Immunohistochemical studies also revealed the presence of MAP-1B-positive staining in interstitial nerve fibers in caput and cauda epididymidis from both rat and human. Thus, the expression of MAP 1B is not confined to a specific cell type in rat and human efferent ductules and epididymis. The functional significance of this cytoskeleton protein in tissues from the male reproductive tract requires further investigation.

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Microtubule-associated protein 2 phosphorylation is decreased in the human epileptic temporal lobe cortex

Cited by 15 publications

References 65 publications

Differential distribution of neurons in the gyral white matter of the human cerebral cortex

Differential distribution of neurons in the gyral white matter of the human cerebral cortex

Differential expression of nuclear matrix proteins during the differentiation of human neuroblastoma SK‐N‐SH cells induced by retinoic acid

Cells positive for microtubule-associated protein 1B (MAP 1B) are present along rat and human efferent ductules and epididymis

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