Microtubule-Associated Kinase-like Protein RUNKEL Needed for Cell Plate Expansion in Arabidopsis Cytokinesis

Krupnova, Tamara; Sasabe, Michiko; Ghebreghiorghis, Luam; Gruber, Christian W.; Hamada, Takahiro; Dehmel, Verena; Strompen, Georg; Stierhof, York‐Dieter; Lukowitz, Wolfgang; Kemmerling, Birgit; Machida, Yasunori; Hashimoto, Takashi; Mayer, Ulríke; Jürgens, Gerd

doi:10.1016/j.cub.2009.02.021

Cited by 44 publications

(17 citation statements)

References 29 publications

Supporting

Mentioning

Contrasting

Order By: Relevance

“…Endosperm defective 1 (AT2G44190; c44482_g1_i1) is a microtubule-associated protein, essential for cell division of embryo and endosperm82. RUNKEL (AT5G18700; c50689_g1_i1) is responsible for phragmoplast organization and cell plate expansion83. ALTERED MERISTEM PROGRAM 1 (AT3G54720; c47383_g5_i1 & c48054_g2_i2) regulates pollen tube attraction by synergid cells84.…”

Section: Resultsmentioning

confidence: 99%

Transcriptional changes during ovule development in two genotypes of litchi (Litchi chinensis Sonn.) with contrast in seed size

Pathak

Singh

Gupta

et al. 2016

Sci Rep

View full text Add to dashboard Cite

Litchi chinensis is a subtropical fruit crop, popular for its nutritional value and taste. Fruits with small seed size and thick aril are desirable in litchi. To gain molecular insight into gene expression that leads to the reduction in the size of seed in Litchi chinensis, transcriptomes of two genetically closely related genotypes, with contrasting seed size were compared in developing ovules. The cDNA library constructed from early developmental stages of ovules (0, 6, and 14 days after anthesis) of bold- and small-seeded litchi genotypes yielded 303,778,968 high quality paired-end reads. These were de-novo assembled into 1,19,939 transcripts with an average length of 865 bp. A total of 10,186 transcripts with contrast in expression were identified in developing ovules between the small- and large- seeded genotypes. A majority of these differences were present in ovules before anthesis, thus suggesting the role of maternal factors in seed development. A number of transcripts indicative of metabolic stress, expressed at higher level in the small seeded genotype. Several differentially expressed transcripts identified in such ovules showed homology with Arabidopsis genes associated with different stages of ovule development and embryogenesis.

show abstract

Section: Resultsmentioning

confidence: 99%

Transcriptional changes during ovule development in two genotypes of litchi (Litchi chinensis Sonn.) with contrast in seed size

Pathak

Singh

Gupta

et al. 2016

Sci Rep

View full text Add to dashboard Cite

show abstract

“…Indeed, previous functional studies of plant PKs indicated that these PK genes within conserved exon-intron structures function as core components of various signaling pathways that control multiple plant cellular processes. (1) Mitosis: Aur [ 51 ] and ULK_ULK4 [ 52 ] function in cell division in A . thaliana .…”

Section: Discussionmentioning

confidence: 99%

Genome-wide classification, evolutionary analysis and gene expression patterns of the kinome in Gossypium

Yan

Guo

et al. 2018

PLoS ONE

View full text Add to dashboard Cite

The protein kinase (PK, kinome) family is one of the largest families in plants and regulates almost all aspects of plant processes, including plant development and stress responses. Despite their important functions, comprehensive functional classification, evolutionary analysis and expression patterns of the cotton PK gene family has yet to be performed on PK genes. In this study, we identified the cotton kinomes in the Gossypium raimondii, Gossypium arboretum, Gossypium hirsutum and Gossypium barbadense genomes and classified them into 7 groups and 122–24 subfamilies using software HMMER v3.0 scanning and neighbor-joining (NJ) phylogenetic analysis. Some conserved exon-intron structures were identified not only in cotton species but also in primitive plants, ferns and moss, suggesting the significant function and ancient origination of these PK genes. Collinearity analysis revealed that 16.6 million years ago (Mya) cotton-specific whole genome duplication (WGD) events may have played a partial role in the expansion of the cotton kinomes, whereas tandem duplication (TD) events mainly contributed to the expansion of the cotton RLK group. Synteny analysis revealed that tetraploidization of G. hirsutum and G. barbadense contributed to the expansion of G. hirsutum and G. barbadense PKs. Global expression analysis of cotton PKs revealed stress-specific and fiber development-related expression patterns, suggesting that many cotton PKs might be involved in the regulation of the stress response and fiber development processes. This study provides foundational information for further studies on the evolution and molecular function of cotton PKs.

show abstract

“…4b–d), suggesting that the AtNACK1-GFP protein has a dominant-inhibitory effect on wild-type AtNACK1 in the transgenic plants. The Arabidopsis mutation of RUNKEL exhibits severe cytokinetic defects and results in seedling lethal (Krupnova et al 2009). Recently, it has been reported that the transgenic plants expressing truncated RUNKEL proteins cause the mislocalization of Myc-AtNACK1/HIK, such that it is dispersed around the cell plate and between the daughter nuclei from early to late stages of cytokinesis in these plants (Krupnova et al 2013).…”

Section: Discussionmentioning

confidence: 99%

The carboxyl-terminal tail of the stalk of Arabidopsis NACK1/HINKEL kinesin is required for its localization to the cell plate formation site

et al. 2014

Self Cite

View full text Add to dashboard Cite

Plant cytokinesis is achieved by formation of cell plates in the phragmoplast, a plant-specific cytokinetic apparatus, which consists of microtubules (MTs) and microfilaments. During cytokinesis, the cell plate is expanded centrifugally outward from the inside of cells in a process that is supported by dynamic turnover of MTs. M-phase-specific kinesin NACK1, which comprises the motor domain at the amino-terminal half to move on MT bundles and the stalk region in the carboxyl-terminal half, is a key player in the process of MT turnover. That is, the specific region in the stalk binds the MAP kinase kinase kinase to activate the whole MAP kinase cascade, which stimulates depolymerization of MTs for the MT turnover. The stalk is also responsible for recruiting the activated kinase cascade to the mid-zone of the phragmoplast, which corresponds to the cell-plate formation site. It should be crucial to uncover roles of the NACK1 kinesin stalk as well as the motor domain in the formation of cell plates in order to understand the mechanisms of cell plate formation. Using dissected Arabidopsis NACK1 (AtNACK1/HINKEL) molecules and AtNACK1-fused GFP, we showed that the C-terminal tail of the stalk in addition to the motor domain is critical for its proper localization to the site of cell plate formation in the phragmoplast, probably by affecting its motility activity.

show abstract

Microtubule-Associated Kinase-like Protein RUNKEL Needed for Cell Plate Expansion in Arabidopsis Cytokinesis

Cited by 44 publications

References 29 publications

Transcriptional changes during ovule development in two genotypes of litchi (Litchi chinensis Sonn.) with contrast in seed size

Transcriptional changes during ovule development in two genotypes of litchi (Litchi chinensis Sonn.) with contrast in seed size

Genome-wide classification, evolutionary analysis and gene expression patterns of the kinome in Gossypium

The carboxyl-terminal tail of the stalk of Arabidopsis NACK1/HINKEL kinesin is required for its localization to the cell plate formation site

Contact Info

Product

Resources

About