Microtubule actin crosslinking factor 1 promotes osteoblast differentiation by promoting β‐catenin/TCF1/Runx2 signaling axis

Hu, Lifang; Su, Peihong; Chen, Yin; Zhang, Yan; Li, Runzhi; Yan, Kun; Chen, Zhihao; Li, Dijie; Zhang, Ge; Wang, Liping; Miao, Zhiping; Qian, Airong; Chen, Xian

doi:10.1002/jcp.26059

Cited by 47 publications

(49 citation statements)

References 40 publications

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“…MACF1 deficiency restrains the translocation of the Axin complex, which results in the disruption of the Wnt‐signaling pathways. MACF1 exhibits a phosphorylation site for GSK‐3β and also regulates GSK‐3 signaling pathway . These conclusions confirm the role of MACF1 in cell signaling and vesicular transport.…”

Section: Physiological Role Of Microtubule Actin Cross‐linking Factorsupporting

confidence: 75%

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Microtubule actin cross‐linking factor 1, a novel potential target in cancer

Miao

Ali

et al. 2017

Cancer Science

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Cancer is a polygenic disease characterized by uncontrolled growth of normal body cells, deregulation of the cell cycle as well as resistance to apoptosis. The spectraplakin protein microtubule actin cross‐linking factor 1 (MACF1) plays an essential function in various cellular processes, including cell proliferation, migration, signaling transduction and embryo development. MACF1 is also involved in processes such as metastatic invasion in which cytoskeleton organization is a critical element that contributes to tumor progression in various human cancers. Aberrant expression of MACF1 initiates the tumor cell proliferation, and migration and metastasis in numerous cancers, such as breast cancer, colon cancer, lung cancer and glioblastoma. In this review, we summarized the current knowledge of MACF1 and its critical role in different human cancers. This will be helpful for researchers to investigate the novel functional role of MACF1 in human cancers and as a potential target to enhance the efficacy of therapeutic treatment modalities.

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Section: Physiological Role Of Microtubule Actin Cross‐linking Factorsupporting

confidence: 75%

“…MACF1 exhibits a phosphorylation site for GSK-3b (19) and also regulates GSK-3 signaling pathway. (28,29) These conclusions confirm the role of MACF1 in cell signaling and vesicular transport. In the case of Axonal vesicle transportation, MACF1 plays a critical role in translocation of vesicles between the Trans-Golgi network and Kif5A.…”

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confidence: 71%

Microtubule actin cross‐linking factor 1, a novel potential target in cancer

Miao

Ali

et al. 2017

Cancer Science

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“…Our previous study found that MACF1 had important regulatory effects on the function of osteoblast by promoting nuclear translocation of β‐catenin, which is essential for osteogenic differentiation (Hu et al, ; Y. Zhang et al, ). We supposed that MACF1 probably regulate osteogenic differentiation through epigenetic or nonepigenetic signaling pathways.…”

Section: Discussionmentioning

confidence: 99%

“…Microtubule‐actin crosslinking factor 1 (MACF1) was a cytoskeletal protein regulating cellular signal transduction, proliferation, migration, and intracellular material transportation (Fassett et al, ; Hu et al, ; Kodama, Karakesisoglou, Wong, Vaezi, & Fuchs, ; X. Wang et al, ; Yue et al, ). Our previous studies have shown that MACF1 positively regulated osteoblast differentiation and osteogenic signaling pathways (Hu et al, ; Yin et al, ; Y. Zhang et al, ). We assume that MACF1 may regulate osteoblast differentiation through a series of classical or epigenetic mechanisms.…”

Section: Introductionmentioning

confidence: 98%

A novel long noncoding RNA AK016739 inhibits osteoblast differentiation and bone formation

Chen

Wang

et al. 2019

Journal Cellular Physiology

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The incidence of postmenopausal osteoporosis research 50% in middle‐aged and older women, however, effects of existing therapy are not ideal. Emerging evidence have proved that long noncoding RNAs (lncRNAs) was correlated with multiple physiological and pathology processes including development, carcinogenesis, and osteogenesis. However, reports on lncRNAs regulating bone formation were relatively limited. In this study, we screened osteogenic lncRNAs through mRNA/lncRNA microarray combined with gene coexpression analysis. The biological function of the screened lncRNA was assessed both in vitro and in vivo. The effects of the lncRNA on osteogenic transcription factors were also evaluated. We identified AK016739, which was correlated with osteogenic differentiation and enriched in skeletal tissues of mice. The expression levels of AK016739 in bone‐derived mesenchymal stem cells were increased with age and negatively correlated with osteogenic differentiation marker genes. Experiments showed that AK016739 inhibited osteoblast differentiation, and in vivo inhibition of AK016739 by its small interfering RNA would rescue bone formation in ovariectomized osteoporosis mice model. In addition, AK016739 suppressed both expression levels and activities of osteogenic transcription factors. This newly identified lncRNA AK016739 has revealed a new mechanism of osteogenic differentiation and provided new targets for treatment of skeletal disorders.

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“…However, the downstream molecular mechanisms for the MACF1 effect in regulating osteoblast proliferation remain unclear. Previously, MACF1 was shown to participate in Wnt/β‐catenin pathway allowing β‐catenin to dissociate from the β‐catenin/AXIN/APC complex and thus transport to cell nucleus (Chen et al, ; Hu et al, ). Here, we found that the expression levels of β‐catenin increased in MACF1‐overexpression MC3T3‐E1 cells, but decreased in MACF1‐knockdown MC3T3‐E1 cells.…”

Section: Discussionmentioning

confidence: 99%

Mechanical unloading reduces microtubule actin crosslinking factor 1 expression to inhibit β‐catenin signaling and osteoblast proliferation

Chen

Zhang

et al. 2018

Journal Cellular Physiology

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Mechanical unloading was considered a major threat to bone homeostasis, and has been shown to decrease osteoblast proliferation although the underlying mechanism is unclear. Microtubule actin crosslinking factor 1 (MACF1) is a cytoskeletal protein that regulates cellular processes and Wnt/β-catenin pathway, an essential signaling pathway for osteoblasts. However, the relationship between MACF1 expression and mechanical unloading, and the function and the associated mechanisms of MACF1 in regulating osteoblast proliferation are unclear. This study investigated effects of mechanical unloading on MACF1 expression levels in cultured MC3T3-E1 osteoblastic cells and in femurs of mice with hind limb unloading; and it also examined the role and potential action mechanisms of MACF1 in osteoblast proliferation in MACF1-knockdown, overexpressed or control MC3T3-E1 cells treated with or without the mechanical unloading condition. Results showed that the mechanical unloading condition inhibited osteoblast proliferation and MACF1 expression in MC3T3-E1 osteoblastic cells and mouse femurs. MACF1 knockdown decreased osteoblast proliferation, while MACF1 overexpression increased it. The inhibitory effect of mechanical unloading on osteoblast proliferation also changed with MACF1 expression levels. Furthermore, MACF1 was found to enhance β-catenin expression and activity, and mechanical unloading decreased β-catenin expression through MACF1. Moreover, β-catenin was found an important regulator of osteoblast proliferation, as its preservation by treatment with its agonist lithium attenuated the inhibitory effects of MACF1-knockdown or mechanical unloading on osteoblast proliferation. Taken together, mechanical unloading decreases MACF1 expression, and MACF1 up-regulates osteoblast proliferation through enhancing β-catenin signaling. This study has thus provided a mechanism for mechanical unloading-induced inhibited osteoblast proliferation.

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Microtubule actin crosslinking factor 1 promotes osteoblast differentiation by promoting β‐catenin/TCF1/Runx2 signaling axis

Cited by 47 publications

References 40 publications

Microtubule actin cross‐linking factor 1, a novel potential target in cancer

Microtubule actin cross‐linking factor 1, a novel potential target in cancer

A novel long noncoding RNA AK016739 inhibits osteoblast differentiation and bone formation

Mechanical unloading reduces microtubule actin crosslinking factor 1 expression to inhibit β‐catenin signaling and osteoblast proliferation

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