MicroTom - A Model Plant System to Study Bacterial Wilt by Ralstonia solanacearum

Chemical Pesticides and Plant Essential Oils for Disease Control of Tomato Bacterial Wilt

“…Twenty ml of pesticide or essential oil solutions were each poured evenly into a pot with one 5-week-old tomato seedling. The same volume of the bacterial suspension (10 8 cfu/ml) of R. solanacearum was also poured into the pot (Park et al, 2007a) to make a bacterial concentration of 10 7 cfu/ml in pot. Disease progress with wilting symptom was monitored daily for 10 days and rated based on the method of Roberts et al (1988): 0, healthy; 1, 1 to 25% leaf area wilted; 2, 26 to 50% leaf are wilted; 3, 51 to 75% leaf area wilted; and 4, > 75% leaf area wilted.…”

Loss of glutamate dehydrogenase in Ralstonia solanacearum alters dehydrogenase activity, extracellular polysaccharide production and bacterial virulence

“…Tomato plants were grown in pots containing commercial horticulture nursery-media soil (Punong Co., Ltd, Korea) and maintained in the plastic house for 6 weeks at 20e30 C. Before inoculation, the plants were moved to a growth chamber and grown at 28 C in light for 14 h and in the dark for 10 h. The virulence of R. solanacearum strains SL341, SL341P2, SL341P2B and SL341P2C was assessed by inoculating 3-4-week-old tomato plants using the soil-soaking method [27]. Bacterial cells were grown in CPG broth and centrifuged at 8000 rpm.…”

“…F -thi-1 hsdS20 (rB À , mB À ) supE44 recA13 ara-14 leuB6 proA2 lacY1 galK2 rpsL20 (str r ) xyl-5 mtl- 1 2 3 4 5 6 7 8 9 10 11 12 13 14 15 16 17 18 19 20 21 22 23 24 25 26 27 28 29 30 31 32 33 34 35 36 37 38 39 40 41 42 43 44 45 46 47 48 49 50 51 52 53 54 55 56 57 58 59 60 61 62 63 64 were conducted as described previously [27]. Tomato plants were grown in pots containing commercial horticulture nursery-media soil (Punong Co., Ltd, Korea) and maintained in the plastic house for 6 weeks at 20e30 C. Before inoculation, the plants were moved to a growth chamber and grown at 28 C in light for 14 h and in the dark for 10 h. The virulence of R. solanacearum strains SL341, SL341P2, SL341P2B and SL341P2C was assessed by inoculating 3-4-week-old tomato plants using the soil-soaking method [27].…”

Synergistic effect of acetyl salicylic acid and DL-Beta-aminobutyric acid on biocontrol efficacy of Bacillus strains against tomato bacterial wilt

“…The second application of treatments was soil drenching with 60 mL per pot of bacterial suspension (1 × 10 7 CFU mL -1 ) or aqueous solution of ASA or BABA (1 mM) or their combinations were poured in each pot one week before inoculation with pathogen (Almoneafy et al, 2012). The plants were weekly observed and disease index was recorded for each treatment according to the method described by Park et al (2007). Disease incidence was then calculated for each treatment after 15, 30, 45 and 60 days post inoculation using the formula (Xue et al, 2009):…”