Microtiter Plate-Format Optode

Kim, Sung‐Bae; Cho, Ho Choll; Sig, Geun; Nam, Hakhyun

doi:10.1021/ac980322+

Cited by 22 publications

(10 citation statements)

References 18 publications

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“…21 An even more promising technique, however, that offers rapid, high-throughput analyses with multiplexing capabilities is flow cytometry. Microspheres have been used for flow cytometric applications for more than 25 years 22 and they are commonly used for multiplexed analyses.…”

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confidence: 99%

“…In an attempt to increase the throughput of ion determinations, Kim et al have described a 96-well plate-format absorbance-based optode that required microvolume samples and that could be read using existing clinical laboratory instrumentation. 21 An even more promising technique, however, that offers rapid, high-throughput analyses with multiplexing capabilities is flow cytometry. Microspheres have been used for flow cytometric applications for more than 25 years 22 and they are commonly used for multiplexed analyses.…”

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confidence: 99%

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Flow Cytometric Ion Detection with Plasticized Poly(Vinyl Chloride) Microspheres Containing Selective Ionophores

et al. 2002

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The use of flow cytometry as a rapid, high-throughput diagnostic tool for the analysis of ions is described. Monodisperse, uniform microspheres, which obey bulk optode theory and are governed by bulk extraction processes rather than surface phenomena, were prepared under mild, nonreactive conditions using a sonic stream particle casting apparatus. As an initial example demonstrating the utility of this approach, microspheres that contained a H+-selective fluorescent chromoionophore (ETH 5294), a cation-exchanger (NaTFPB), and either a highly sodium-selective (sodium ionophore X) or a potassium-selective ionophore (BME-44) were prepared. Separate solution analysis of sodium- and potassium-selective microspheres resulted in the generation of functional response curves using peak channel fluorescence intensities. The selectivity observed for both types of particles is sufficient for the clinical determination of Na+ and K+. Furthermore, sodium- and potassium-selective microspheres were analyzed in parallel using sodium sample solutions, resulting in the successful determination of sodium ion concentrations and providing important information about the selectivity of the potassium-selective sensors over sodium. This work demonstrates the potential applicability of flow cytometry as a means for developing multiplexed, rapid, high-throughput analyses for clinically relevant ions.

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Flow Cytometric Ion Detection with Plasticized Poly(Vinyl Chloride) Microspheres Containing Selective Ionophores

et al. 2002

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“…To prepare the calcium selective nanosensors, 1.9 mg of NaTFPB, 1.4 mg of Chromoionophore I, 8.0 mg of DOS, 5.0 mg of F127, and 6.5 mg of calcium ionophore IV were dissolved in 3.0 mL of THF to form a homogeneous solution. A 0.5-mL portion of this solution was pipetted and injected into 4.5 mL of deionized water on a vortex with a spinning speed of 1000 r/min.…”

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confidence: 99%

Ionophore-Based Ion-Selective Optical NanoSensors Operating in Exhaustive Sensing Mode

et al. 2014

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Ion selective optical sensors are typically interrogated under conditions where the sample concentration is not altered during measurement. We describe here an alternative exhaustive detection mode for ion selective optical sensors. This exhaustive sensor concept is demonstrated with ionophore-based nanooptodes either selective for calcium or the polycationic heparin antidote protamine. In agreement with a theoretical treatment presented here, linear calibration curves were obtained in the exhaustive detection mode instead of the sigmoidal curves for equilibrium-based sensors. The response range can be tuned by adjusting the nanosensor loading. The nanosensors showed average diameters of below 100 nm and the sensor response was found to be dramatically faster than that for film-based optodes. Due to the strong binding affinity of the exhaustive nanosensors, total calcium concentration in human blood plasma was successfully determined. Optical determination of protamine in human blood plasma using the exhaustive nanosensors was attempted, but was found to be less successful.

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“…In contrast to microelectrodes, they are easy to manufacture, insensitive to stirring, do not consume oxygen and show fairly long-term stability (Klimant et al, 1995). Optodes can be introduced into a variety of closed and flow-through (Sanchez-Pedreno et al, 2000) incubation chambers or even in microtiter plate-format (Kim et al, 1998). They are suited for experimentation under conditions in which conventional chemical analysis or use of polarographic electrodes is difficult, e.g.…”

Section: Optodesmentioning

confidence: 99%