Microtissues and tridimensional cell models: A new paradigm in tissue engineering

Alves, Gutemberg Gomes; Costa-Silva, Daniela

doi:10.4103/gfsc.gfsc_8_19

Cited by 2 publications

(3 citation statements)

References 0 publications

Supporting

Mentioning

Contrasting

Order By: Relevance

“…Alkaline phosphatase activity, an important biomarker of early osteoblasts response, similarly increased in the FBS-treated groups (even after removal of background activity), with the higher levels observed with PLA-CaP+FBS. The increase in ALP activity may indicate a greater response to cell differentiation capacity for PLA-CaP scaffold with FBS and is in accordance with recent results of studies with HA-coated PLA scaffolds in contact with mesenchymal stem cells [ 84 ]. The present findings provide insight into the possible role of the biomimetic coating in PLA and the interface with such proteins, resulting in increased interactions with cells.…”

Section: Discussionsupporting

confidence: 90%

“…For the initial assessment of such biological responses, the present study employed an in vitro tridimensional model based on a spheroidal 3D culture of osteoblastic cells, as proposed previously [ 49 ]. The use of tridimensional cell aggregates can increase the similarity with the microenvironment present on in vivo tissues, with more information than 2D cells models [ 84 , 85 ], as cells can remain integrated into the spheroid or migrate and proliferate to colonize a material surface, depending on its osteoconductive properties. The primary human osteoblast spheroids also allow the focal seeding of cells in a specific point of contact, providing insights on the migration and extent of occupation of a material surface over time.…”

Section: Discussionmentioning

confidence: 99%

See 1 more Smart Citation

Biomimetic Mineralization on 3D Printed PLA Scaffolds: On the Response of Human Primary Osteoblasts Spheroids and In Vivo Implantation

et al. 2020

View full text Add to dashboard Cite

This study aimed to assess the response of 3D printed polylactic acid (PLA) scaffolds biomimetically coated with apatite on human primary osteoblast (HOb) spheroids and evaluate the biological response to its association with Bone Morphogenetic Protein 2 (rhBMP-2) in rat calvaria. PLA scaffolds were produced via 3D printing, soaked in simulated body fluid (SBF) solution to promote apatite deposition, and characterized by physical-chemical, morphological, and mechanical properties. PLA-CaP scaffolds with interconnected porous and mechanical properties suitable for bone repairing were produced with reproducibility. The in vitro biological response was assessed with human primary osteoblast spheroids. Increased cell adhesion and the rise of in vitro release of growth factors (Platelet-Derived Growth Factor (PDGF), Basic Fibroblast Growth Factor (bFGF), Vascular Endothelial Growth Factor (VEGF) was observed for PLA-CaP scaffolds, when pre-treated with fetal bovine serum (FBS). This pre-treatment with FBS was done in a way to enhance the adsorption of serum proteins, increasing the number of bioactive sites on the surface of scaffolds, and to partially mimic in vivo interactions. The in vivo analysis was conducted through the implantation of 3D printed PLA scaffolds either alone, coated with apatite (PLA-CaP) or PLA-CaP loaded with rhBMP-2 on critical-sized defects (8 mm) of rat calvaria. PLA-CaP+rhBMP2 presented higher values of newly formed bone (NFB) than other groups at all in vivo experimental periods (p < 0.05), attaining 44.85% of NFB after six months. These findings indicated two new potential candidates as alternatives to autogenous bone grafts for long-term treatment: (i) 3D-printed PLA-CaP scaffold associated with spheroids, since it can reduce the time of repair in situ by expression of biomolecules and growth factors; and (ii) 3D-printed PLA-CaP functionalized rhBMP2 scaffold, a biocompatible, bioactive biomaterial, with osteoconductivity and osteoinductivity.

show abstract

Section: Discussionsupporting

confidence: 90%

Section: Discussionmentioning

confidence: 99%

Biomimetic Mineralization on 3D Printed PLA Scaffolds: On the Response of Human Primary Osteoblasts Spheroids and In Vivo Implantation

et al. 2020

View full text Add to dashboard Cite

show abstract

“…For the initial assessment of such biological responses, the present study employed an in vitro tridimensional model based on a spheroidal 3D culture of osteoblastic cells, as proposed previously [49]. The use of tridimensional cell aggregates can increase the similarity with the microenvironment present on in vivo tissues, with more information than 2D cells models [84,85], as cells can remain integrated into the spheroid or migrate and proliferate to colonize a material surface, depending on its osteoconductive properties. The primary human osteoblast spheroids also allow the focal seeding of cells in a specific point of contact, providing insights on the migration and extent of occupation of a material surface over time.…”

Section: Discussionmentioning

confidence: 99%

Biomimetic Mineralization on 3d Printed Pla Scaffolds: On the Response of Human Primary Osteoblasts Spheroids and in Vivo Implantation

Maia-Pinto

Brochado

Teixeira

et al. 2020

Preprint

View full text Add to dashboard Cite

This study aimed to assess the response of 3D printed PLA scaffolds biomimetically coated with apatite on human primary osteoblast spheroids and evaluate the biological response to its association with Bone Morphogenetic Protein 2 (rhBMP-2) in rat calvaria. PLA scaffolds were produced via 3D printing, soaked in simulated body fluid (SBF) solution, and characterized by physical-chemical, morphological, and mechanical properties. The in vitro biological response was assessed with human primary osteoblast (HOb) spheroids. The in vivo analysis was conducted through the implantation of 3D printed PLA scaffolds either alone, covered by apatite (PLA-CaP) or PLA-CaP loaded with rhBMP-2 (PLA-CaP+rhBMP-2) on critical-sized defects (8 mm) of rat calvaria. Increased cell adhesion and in vitro release of growth factors (PDGF, bFGF, VEGF) was observed for PLA-CaP scaffolds when pre-treated with FBS. PLA-CaP+BMP2 presented higher values of newly formed bone (NFB) than other groups at all experimental periods (p&lt;0.05), attaining 44.85% of NFB after 6 months. These findings indicate that functionalization of PLA scaffolds with biomimetic apatite can improve its biological properties in the presence of complex biological media. Its association with BMP2 may enhance bone repair, suggesting this strategy as a promising candidate for bone tissue engineering.

show abstract

Microtissues and tridimensional cell models: A new paradigm in tissue engineering

Cited by 2 publications

References 0 publications

Biomimetic Mineralization on 3D Printed PLA Scaffolds: On the Response of Human Primary Osteoblasts Spheroids and In Vivo Implantation

Biomimetic Mineralization on 3D Printed PLA Scaffolds: On the Response of Human Primary Osteoblasts Spheroids and In Vivo Implantation

Biomimetic Mineralization on 3d Printed Pla Scaffolds: On the Response of Human Primary Osteoblasts Spheroids and in Vivo Implantation

Contact Info

Product

Resources

About