Microtensile Bond Strength of Composite Resin Following the Use of Bromelain and Papain as Deproteinizing Agents on Etched Dentin: An In Vitro Study

Khatib, Mohd Sibghatullah; Devarasanahalli, Swapna V; Aswathanarayana, Ranjini M; Venkateswara, Ashwath H; Nadig, Roopa R

doi:10.5005/jp-journals-10005-1743

Cited by 4 publications

(7 citation statements)

References 23 publications

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“…In our results CHX showed significant increase in gap leading to increased nanoleakage after thermocycling and this could be explained by the chlorhexidine pretreatment of etch-and-rinse adhesive bonded to dentine failed to prevent hybrid layer degradation after thermocycling (8) . Although the application of chlorhexidine with a etch-and rinse technique on etched dentine surfaces prevented the collagen matrices from collapsing, it caused incomplete water removal from the interfibrillar collagen matrix rendered to the chlorhexidine and was dissolved by water that functioned as the desorption medium (18,27) .…”

Section: Discussionmentioning

confidence: 54%

“…In the current study thermocycling resulted in decreasing the µTBS for only control group. This may be attributed to fluctuation in temperatures during thermocycling, leading to accumulation of thermal stress at the adhesive interface (14,15) . Additionally, HEMA presented in the total etch adhesive is hydrophilic and more liable for water degradation during water storage and thermocycling (14,15) .…”

Section: Discussionmentioning

confidence: 99%

“…This may be attributed to fluctuation in temperatures during thermocycling, leading to accumulation of thermal stress at the adhesive interface (14,15) . Additionally, HEMA presented in the total etch adhesive is hydrophilic and more liable for water degradation during water storage and thermocycling (14,15) . On the other hand, groups treated with MMPs inhibitors and NaOCl showed an insignificant decrease in microtensile bond after 2500 thermocycles.…”

Section: Discussionmentioning

confidence: 99%

“…Specimens were sputtered with gold for scanning electron microscopy (SEM) examination. SEM was used to examine the hybrid layer of teeth samples to evaluate the depth of resin penetration, before and after thermocycling degradation (8) .…”

Section: Evaluation Of Resin-dentine Interface and Resin Tagsmentioning

confidence: 99%

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Evaluation of Matrix Metalloproteinases Biodegradation Inhibition Protocols on Resin-Dentine Interfacial Characteristics (An In Vitro Comparative Study)

EL-Behairy,

ELGhzawy,

Abdalla

2024

Al-Azhar Journal of Dental Science

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Objective: to evaluate and compare the effect of different matrix metalloproteinases biodegradation inhibition protocols. Material and methods: 192 permanent molar sound teeth were collected. Using a diamond disk, wet grinding of the occlusal surface of all samples into flat surface to expose the superficial dentinal surface. 37% phosphoric acid was applied for 15 seconds, cleaned with water and dried with cotton rolls. Then, the molar teeth were divided into four main groups (n=48); group I; No treatment (control group), group II; Chlorhexidine (2%); group III; Minocycline (2%); group IV; Sodium Hypochlorite (5.25%). Samples were cleansed with distilled water and the adhesive bonding of the dentine surface was performed and restored with light cure nanohybrid resin composite. Samples were longitudinally sectioned perpendicular to the adhesive interfaces and prepared according to test before and after thermocycling. Results: For microtensile bond test, control group and minocycline showed highest significant microtensile bond strength in comparison with chlorohexidine and sodium hypochlorite immediately and after thermocycling. While no significant difference was found between different groups for failure mode analysis. For nanoleakage, control group and minocycline showed the least nanoleakage. For hybrid layer resin tag length evaluation control group, minocycline and chlorohexidine groups showed almost similar resin tag penetration while sodium hypochlorite showed the highest penetration for resin tags. Conclusions: Usage of minocycline matrix metalloproteinase inhibitor showed promising results concerning bond strength and nanoleakage on contrary to chlorohexidine. Sodium hypochlorite results showed decreased bond strength, increased nanoleakage and resin tag length.

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Section: Discussionmentioning

confidence: 54%

Section: Discussionmentioning

confidence: 99%

Section: Discussionmentioning

confidence: 99%

Section: Evaluation Of Resin-dentine Interface and Resin Tagsmentioning

confidence: 99%

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Evaluation of Matrix Metalloproteinases Biodegradation Inhibition Protocols on Resin-Dentine Interfacial Characteristics (An In Vitro Comparative Study)

EL-Behairy,

ELGhzawy,

Abdalla

2024

Al-Azhar Journal of Dental Science

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“…Sample size calculation was done by power analysis used microtensile bond strength as the primary outcome. The effect size f = 0.5695921 was calculated based upon the results of Khatib et al 2020, (13) and assuming that the standard deviation within each group = 4.5, using alpha level of 5% and Beta level of 80% i.e. power = 80%.…”

Section: Sample Size Calculationmentioning

confidence: 99%

Effect of Two Different Deproteinizing Agents on Microtensile Bond Strength between Resin Composite and Deep Dentin using Two Restorative Protocols. An In-Vitro Study

Ayman,

Moussa,

Gomaa

et al. 2024

Egyptian Dental Journal

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The purpose of this study was to evaluate the microtensile bond strength of resin composite to deep dentin using two different restorative protocols.Materials and Methods: Forty-five sound permanent molars were used to expose deep dentin. Teeth were etched with 37% phosphoric acid then rinsed. Teeth were divided into five equal groups (n=9) according to deproteinizing method. Group 1: only etching (control), Group 2 and 3: deproteinized with 10% sodium hypochlorite Group 4 and 5: deproteinized with 10% bromelain enzyme. Teeth were restored with two restorative protocols either: packable nanohybrid bulk-fill resin composite or bulk-fill flowable and packable bulk-fil nanohybrid resin composite. Teeth were stored in distilled water (37°C/24 hours) then sectioned into beams. The beams were subjected to microtensile bond strength testing using a universal testing machine at a crosshead speed of 0.5mm/ min until failure occurred.Results: There was a statistically significant difference (p<0.05) between microtensile bond strength values of different groups. Group 1 recorded the highest microtensile bond strength with non-statistically significant difference from Group 4 but a statistically significantly higher microtensile bond strength than other groups. Group 5 recorded the lowest mean microtensile bond strength. Conclusion:Deep Dentin deproteinization, either with 10% sodium hypochlorite solution or 10% bromelain enzyme solution, has no improvement effect on microtensile bond strength with the packable bulk-fill resin composite used in this study. Application of flowable bulk-fill resin composite, as a liner, has a deterioration effect on the microtensile bond strength of deproteinized deep dentin to packable bulk-fill resin composite.

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Collagen-depletion strategies in dentin as alternatives to the hybrid layer concept and their effect on bond strength: a systematic review

Delgado

Costa

Polido

et al. 2022

Sci Rep

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Strategies aiming to improve the longevity of resin–dentin adhesive interface developed so far have only been able to retard the problem. Different approaches are thus needed. The objective of this review was to determine whether the use of collagen-depletion strategies after acid-etching procedures may improve the bond strength of resin-based materials to dentin. A systematic review was planned following 2021 PRISMA statement guidelines, with a search strategy performed in five electronic databases: PubMed/Medline, Scopus, EMBASE, SciELO and IADR Abstract Archive (last search: 17/01/2022). Inclusion criteria encompassed studies which evaluated a collagen-depletion strategy in acid-etched human dentin and tensile/shear bond strength tests. Risk of bias assessment was carried out by two reviewers, working independently on an adapted five-domain risk of bias (RoB) checklist for laboratory studies. Results were synthesized qualitatively, as a meta-analysis was not possible due to limited number of studies and their RoB. A total of eight studies were eligible for inclusion in the systematic review after inclusion/exclusion criteria application. Out of these, two evaluated the effect of using NaOCl followed by an antioxidant, and the remaining six evaluated different enzymatic treatments (bromelain, chondroitinase ABC, papain, and trypsin). None of the studies reported a decrease of bond strength when a collagen-depletion strategy was used, in comparison to traditional hybrid layers (control). All enzymatic treatment studies which respected the inclusion criteria improved the bond strength to dentin. Some specific collagen-depletion strategies seem to play a favorable role in improving immediate bond strengths to dentin. Further research with sound methodology is required to consolidate these findings, since limitations in RoB and a low number of studies were found. The assessment of further proteolytic agents and long-term outcomes is also required.

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Microtensile Bond Strength of Composite Resin Following the Use of Bromelain and Papain as Deproteinizing Agents on Etched Dentin: An In Vitro Study

Cited by 4 publications

References 23 publications

Evaluation of Matrix Metalloproteinases Biodegradation Inhibition Protocols on Resin-Dentine Interfacial Characteristics (An In Vitro Comparative Study)

Evaluation of Matrix Metalloproteinases Biodegradation Inhibition Protocols on Resin-Dentine Interfacial Characteristics (An In Vitro Comparative Study)

Effect of Two Different Deproteinizing Agents on Microtensile Bond Strength between Resin Composite and Deep Dentin using Two Restorative Protocols. An In-Vitro Study

Collagen-depletion strategies in dentin as alternatives to the hybrid layer concept and their effect on bond strength: a systematic review

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