Microsurgical Techniques of Heterotopic and Orthotopic Small-Bowel Transplantation in the Rat

Preissner, W. Ch.; Schröeder, Peter; Gundlach, M.; Liedgens, P.; Schröder, E.; Deltz, E.

doi:10.1007/978-3-642-71087-2_1

Cited by 14 publications

(6 citation statements)

References 5 publications

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“…Bei insgesamt 78 Tieren erfolgte eine isogene, heterotope Dfinndarmtransplantation, die von Preissner und Mitarb. [7] A. Dasselbe gilt ffir alle Transplantate beider Gruppen, bei denen die Transplantation lfinger als zwei Wochen zurfickliegt.…”

Section: Materials Und Methodeunclassified

Untersuchungen zur Regeneration des Lymphabflusses nach D�nndarmtransplantation

Schmid

Körözsi

Klima

et al. 1989

Langenbecks Arch Chiv

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Since the resorption of long-chained fatty acids proceeds exclusively via the lymphatics, the regeneration of lymphatic flow from small bowel grafts takes on a significant role. An isogene, heterotopic small bowel transplant was performed in a total of 54 Lewis rats, whereby 27 animals received no immunosuppression (group A) and the remaining 27 rats received cyclosporin A (group B). The lymphatic regeneration was determined by instilling dye into the mesenterial lymph nodes of the graft between day 1 and day 42 after transplantation and measuring the interval between instillation and appearance in the retroperitoneal lymph channels of the host. Additionally, the severity of the lymph edema was examined in histological slides, and the lymph vessels of the villi were examined for the presence of chylomicrons. In group A the passage of the dye into the lymphatic system first occurred on day 3 after transplantation, namely after a mean of 11.8 min, while in group B the lymph channels of the retroperitoneum first became visible on day 7 after transplantation (mean 8 min after instillation). Passage of the dye out of the lymphatic system of the graft was prompt and unhindered from day 10 in group A and from day 14 after transplantation in group B. In all grafts there was a marked interstitial lymph edema during the first week, and it was less severe in both groups up to day 14. Chylomicrons in the villi were observed in group A as of day 10 and after two weeks in group B. Regeneration of the lymphatics in small bowel grafts thus appears to be adequate for enteral nutrition only after 14 days.

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Section: Materials Und Methodeunclassified

Untersuchungen zur Regeneration des Lymphabflusses nach D�nndarmtransplantation

Schmid

Körözsi

Klima

et al. 1989

Langenbecks Arch Chiv

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“…Orthotopic small bowel transplants using microsurgical techniques were performed in rats [8]. The entire small intestine from the ligament of Treitz to the ileocecal valve was harvested from inbred male Lewis (LEW) rats weighing between 200 and 270 g and trans planted into inbred male LEW rats of the same weight (all animals were obtained from the Zentralinstitut fur Versuchstierzucht.…”

Section: Methodsmentioning

confidence: 99%

Preservation of Small Bowel Grafts – A Comparison with Two Standard Solutions

Thaler

Oberhuber²,

Klima³

et al. 1993

Eur Surg Res

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This study investigates the use of EuroCollins (EC) and University of Wisconsin (UW) solution, two major preservation fluids, for small bowel preservation. After in situ flushing, grafts were cold-stored at 4°C with either EC for 30 min (group 1a), 6 h (group 1b) and 12 h (group 1c) or with UW for 30 min (group 2a), 6 h (group 2b) and 12 h (group 2c). Using UW, cold ischemia was extended to up to 18 h (group 2d). As a control, small intestines were flushed and stored for the same time periods in cold saline (group 3a–c). Survival in group 1b was 66 % versus 100% in group 2b. After 12 h 80% survived in group 2c, but there were no survivors in group 1 c. After 18 h of cold storage, survival was only observed in group 2d (25%). Saline was ineffective after 6 h of preservation. Histology at the end of preservation revealed characteristic changes for EC (intracellular vacuoles) and UW (amorphic granules). We conclude that with UW small bowel can be preserved for up to 12 h.

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“…Accessory small bowel transplantation was per formed in rats using microsurgical techniques as de scribed previously [17,18], The animals were divided into five groups. Group 1 (n = 9): Brown Norway strain rat (BN) recipients received grafts from BN donors and no immunosup pression.…”

Section: Methodsmentioning

confidence: 99%

Small Bowel Transplantation in the Rat: Impact of Various Immunosuppressive Regimens on Graft-versus-Host Reaction

Oberhuber

Schmid²,

Erdkönig³

et al. 1991

Eur Surg Res

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The effect of ciclosporin (CS) and methotrexate (MTX) on the development of graft-versus-host (GvH) disease was examined after small bowel allotransplantation in the rat. The drugs were tested either alone or in combination. Lewis small bowel allografts were transplantated into Brown Norway recipients in a heterotopic position. The native small bowel, spleen, liver, skin, mesenteric lymph nodes and the kidney of the recipients were examined histologically 5, 10 and 20 days after allotransplantation. Intraepithelial lymphocyte numbers were determined quantitatively in the native small bowel. The relative spleen weight of the host was detemined after sacrifice for estimation of the severity of GvH disease. Grade I GvH reaction of the native small bowel occurred in the animals without immunosuppression, but graft rejection predominated in this group. Treatment with CS was effective in the early postoperative period; after 10 and 20 days GvH lesions in the native small bowel were comparable to those observed in the allogeneic combinations. MTX had a detrimental effect on the allografts and the GvH reaction was augmented. When CS and MTX were combined, GvH lesions were comparable to those in the animals treated solely with CS. Animals, however, suffered from heavy side effects. The spleen, liver, lymph nodes and kidney exhibited only unspecific histologic changes, which could not unequivocally be recognized as a GvH reaction. This was true for all groups. As a conclusion it can be said that GvH reaction occurs in the early postoperative period in a fully allogeneic model and cannot be prevented by CS in the dosae used. MTX was not seen to be of any value in this regard.

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Microsurgical Techniques of Heterotopic and Orthotopic Small-Bowel Transplantation in the Rat

Cited by 14 publications

References 5 publications

Untersuchungen zur Regeneration des Lymphabflusses nach D�nndarmtransplantation

Untersuchungen zur Regeneration des Lymphabflusses nach D�nndarmtransplantation

Preservation of Small Bowel Grafts – A Comparison with Two Standard Solutions

Small Bowel Transplantation in the Rat: Impact of Various Immunosuppressive Regimens on Graft-versus-Host Reaction

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Microsurgical Techniques of Heterotopic and Orthotopic Small-Bowel Transplantation in the Rat

Cited by 14 publications

References 5 publications

Untersuchungen zur Regeneration des Lymphabflusses nach D�nndarmtransplantation

Untersuchungen zur Regeneration des Lymphabflusses nach D�nndarmtransplantation

Preservation of Small Bowel Grafts &ndash; A Comparison with Two Standard Solutions

Small Bowel Transplantation in the Rat: Impact of Various Immunosuppressive Regimens on Graft-versus-Host Reaction

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Preservation of Small Bowel Grafts – A Comparison with Two Standard Solutions