MicroSugar: A database of comprehensive miRNA target prediction framework for sugarcane (Saccharum officinarum L.)

Qiu, Lihang; Luo, Hanmin; Zhou, Huiwen; Yan, Haifeng; Fan, Yanting; Zhou, Zhongfeng; Chen, Rongfa; Liu, Junxian; Luo, Ting; Deng, Yuchi; Xiong, Fei; Wu, Jianming

doi:10.1016/j.ygeno.2022.110420

Cited by 1 publication

(2 citation statements)

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“…N is an essential macro nutrient element for crops, and it is an important component of nucleic acid, phospholipid, and protein [ 1 ]. Sugarcane is the most important sugar crop [ 2 , 3 ]. N is one of the limiting factors for cane tonnage.…”

Section: Introductionmentioning

confidence: 99%

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Identification of Low-Nitrogen-Related miRNAs and Their Target Genes in Sugarcane and the Role of miR156 in Nitrogen Assimilation

Gao

Yang

et al. 2022

IJMS

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Chemical nitrogen (N) fertilizer is widely used in sugarcane production, especially in China and India. Understanding the molecular mechanisms and mining miRNAs and their target genes associated with nitrogen use efficiency (NUE) in sugarcane can aid in developing the N-efficient varieties, and thus is beneficial to reduce N fertilizer application. In this study, the root miRNA database of N-efficient sugarcane variety ROC22 under low N stress (0.3 mM NH4NO3) for 3 h was constructed, along with their transcriptome-rearranged data. KEGG analysis indicated that those candidate target genes, corresponding to differentially expressed miRNAs, were mainly enriched in N metabolism, amino acid metabolism, carbohydrate metabolism, photosynthesis, and hormone signal transduction pathways. It was found that under low N stress for 0–24 h, there was a negative correlation between miR168 and SPX, along with miR396 and acnA. Furthermore, the expression of miR156 in the roots of ROC22 was significantly up-regulated under low N treatment. Compared with the wild-type, the Arabidopsis plants overexpressing sugarcane miR156 exhibited significantly improved length and surface area of roots, while the expression of one NO3- transporter gene NRT1.1, three N assimilation key genes (NR1, NIR1, and GS), and the activity of two N assimilation key enzymes (NR and GS) were up-regulated under low N treatment. It can be reasonably deduced that sugarcane miR156 can enhance the nitrogen assimilation ability of the overexpressed Arabidopsis plants under low N application, and thus has a potential ability for improving sugarcane NUE. The present study should be helpful for understanding the molecular regulatory network in the N-efficient sugarcane genotype responding to low N stress and could provide the candidate miRNAs with a potential function in improving sugarcane NUE.

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Section: Introductionmentioning

confidence: 99%

“…In many biological metabolic processes, especially in stress response, miRNAs play an important regulatory role by inducing or inhibiting gene expression [ 3 , 6 , 7 ]. As reported, miRNAs play an important role in the regulation of plant nutrition [ 8 , 9 , 10 ].…”

Section: Introductionmentioning

confidence: 99%