Microstructured hydrogel scaffolds containing differential density interfaces promote rapid cellular invasion and vascularization

Celie, Karel-Bart; Toyoda, Yoshiko; Dong, Xue; Morrison, Kerry A.; Zhang, Peipei; Asanbe, Ope; Jin, Julia; Hooper, Rachel C.; Zanotelli, Matthew R.; Kaymakcalan, Omer; Bender, Ryan J.; Spector, Jason A.

doi:10.1016/j.actbio.2019.04.027

Cited by 17 publications

(14 citation statements)

References 64 publications

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“…[ 1,2 ] It can be applied in the form of film, [ 3,4 ] hydrogel, [ 5,6 ] or sponge, [ 7,8 ] among which hydrogels are of preference in a large range of medical applications due to the moisture‐maintaining capacity and structural similarity to extracellular matrix. [ 1 ] For instance, collagen hydrogels have been used as tissue engineered devices for the regeneration of skin, [ 9,10 ] bone, [ 11 ] cartilage, [ 12 ] intervertebral disc, [ 13 ] heart, [ 5 ] etc. In many reported cases, collagen hydrogels were fabricated from low concentrated solutions and suffered from several limitations such as poor mechanical properties and fast degradability.…”

Section: Introductionmentioning

confidence: 99%

Nanostructured Dense Collagen‐Polyester Composite Hydrogels as Amphiphilic Platforms for Drug Delivery

et al. 2021

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Associating collagen with biodegradable hydrophobic polyesters constitutes a promising method for the design of medicated biomaterials. Current collagen‐polyester composite hydrogels consisting of pre‐formed polymeric particles encapsulated within a low concentrated collagen hydrogel suffer from poor physical properties and low drug loading. Herein, an amphiphilic composite platform associating dense collagen hydrogels and up to 50 wt% polyesters with different hydrophobicity and chain length is developed. An original method of fabrication is disclosed based on in situ nanoprecipitation of polyesters impregnated in a pre‐formed 3D dense collagen network. Composites made of poly(lactic‐co‐glycolic acid) (PLGA) and poly(lactic acid) (PLA) but not polycaprolactone (PCL) exhibit improved mechanical properties compared to those of pure collagen dense hydrogels while keeping a high degree of hydration. Release kinetics of spironolactone, a lipophilic steroid used as a drug model, can be tuned over one month. No cytotoxicity of the composites is observed on fibroblasts and keratinocytes. Unlike the incorporation of pre‐formed particles, the new process allows for both improved physical properties of collagen hydrogels and controlled drug delivery. The ease of fabrication, wide range of accessible compositions, and positive preliminary safety evaluations of these collagen‐polyesters will favor their translation into clinics in wide areas such as drug delivery and tissue engineering.

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Section: Introductionmentioning

confidence: 99%

Nanostructured Dense Collagen‐Polyester Composite Hydrogels as Amphiphilic Platforms for Drug Delivery

et al. 2021

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“…[ 198–200 ] Differential density interfaces (denser collagen microspheres embedded in a softer collagen bulk matrix) can be created to facilitate robust EC invasion and assembly. [ 59 ] However, these approaches yield nanoporous matrices, which can make it challenging and slow for encapsulated ECs to tunnel through and form mature cell–cell junctions. This problem can be overcome by employing granular hydrogels composed of microporous annealed hydrogel particles (MAPs) or sub‐millimeter modularly assembled hydrogel rods covered with ECs.…”

Section: Fabrication Strategies To Generate Microfluidic and Vascularmentioning

confidence: 99%

Biofabrication Strategies and Engineered In Vitro Systems for Vascular Mechanobiology

Pradhan

Banda

Farino

et al. 2020

Adv Healthcare Materials

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The vascular system is integral for maintaining organ‐specific functions and homeostasis. Dysregulation in vascular architecture and function can lead to various chronic or acute disorders. Investigation of the role of the vascular system in health and disease has been accelerated through the development of tissue‐engineered constructs and microphysiological on‐chip platforms. These in vitro systems permit studies of biochemical regulation of vascular networks and parenchymal tissue and provide mechanistic insights into the biophysical and hemodynamic forces acting in organ‐specific niches. Detailed understanding of these forces and the mechanotransductory pathways involved is necessary to develop preventative and therapeutic strategies targeting the vascular system. This review describes vascular structure and function, the role of hemodynamic forces in maintaining vascular homeostasis, and measurement approaches for cell and tissue level mechanical properties influencing vascular phenomena. State‐of‐the‐art techniques for fabricating in vitro microvascular systems, with varying degrees of biological and engineering complexity, are summarized. Finally, the role of vascular mechanobiology in organ‐specific niches and pathophysiological states, and efforts to recapitulate these events using in vitro microphysiological systems, are explored. It is hoped that this review will help readers appreciate the important, but understudied, role of vascular‐parenchymal mechanotransduction in health and disease toward developing mechanotherapeutics for treatment strategies.

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“…Such hydrogels are formed by covalent or ionic crosslinking of diverse polymers that are biocompatible to easily encapsulate living cells or drug compounds. In a study, a microsphere hydrogel was fabricated by 1% collagen microspheres into a 0.3% collagen bulk for dermal regeneration [64]. The elastic properties and swelling ability of hydrogels makes them suitable for injectable purposes and bio-printing uses, which is a developing technology for the 3D production of structures utilized for the fabrication of complex functional tissues and artificial organs, from nano- to macro-scales.…”

Section: Various Forms Of Scaffolds For Tissue Engineering Applicamentioning

confidence: 99%

Status of Plant Protein-Based Green Scaffolds for Regenerative Medicine Applications

Jahangirian

Azizi²,

Rafiee-Moghaddam

et al. 2019

Biomolecules

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In recent decades, regenerative medicine has merited substantial attention from scientific and research communities. One of the essential requirements for this new strategy in medicine is the production of biocompatible and biodegradable scaffolds with desirable geometric structures and mechanical properties. Despite such promise, it appears that regenerative medicine is the last field to embrace green, or environmentally-friendly, processes, as many traditional tissue engineering materials employ toxic solvents and polymers that are clearly not environmentally friendly. Scaffolds fabricated from plant proteins (for example, zein, soy protein, and wheat gluten), possess proper mechanical properties, remarkable biocompatibility and aqueous stability which make them appropriate green biomaterials for regenerative medicine applications. The use of plant-derived proteins in regenerative medicine has been especially inspired by green medicine, which is the use of environmentally friendly materials in medicine. In the current review paper, the literature is reviewed and summarized for the applicability of plant proteins as biopolymer materials for several green regenerative medicine and tissue engineering applications.

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Microstructured hydrogel scaffolds containing differential density interfaces promote rapid cellular invasion and vascularization

Cited by 17 publications

References 64 publications

Nanostructured Dense Collagen‐Polyester Composite Hydrogels as Amphiphilic Platforms for Drug Delivery

Nanostructured Dense Collagen‐Polyester Composite Hydrogels as Amphiphilic Platforms for Drug Delivery

Biofabrication Strategies and Engineered In Vitro Systems for Vascular Mechanobiology

Status of Plant Protein-Based Green Scaffolds for Regenerative Medicine Applications

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