Microstripes for transport and separation of magnetic particles

Donolato, Marco; Dalslet, Bjarke Thomas; Hansen, Mikkel Fougt

doi:10.1063/1.4704520

Cited by 26 publications

(35 citation statements)

References 16 publications

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“…Figure 3a were made with approximately 1 mg of magnetic material and thus do not provide insight into variations in the properties of individual beads. 15 Variations in the magnetic susceptibility of the SPM beads are quite important in this study as they could limit the precision and efficiency of FNLM separation. Microscopic measurements of magnetophoretic velocity using HGM were used to precisely determine the physical properties of individual beads.…”

Section: Resultsmentioning

confidence: 99%

“…In this article, we demonstrate that FNLM can be used to separate SPM beads with high-resolution based on their magnetic susceptibility. We first describe the preparation and characterization of three SPM beads that are similar in size but have significantly different magnetic 15 properties. We characterize the NLM transport behaviour of these beads on the microscopic scale to define the resolution and practical limitations of FNLM separation.…”

Section: Introductionmentioning

confidence: 99%

“…By using a similar technique, the effective manipulation and 55 separation of very large amount of SPM beads has also been demonstrated. 15 A SPM bead of specific size and magnetization has a critical frequency, ω c , at which it can no longer follow the external magnetic field because the hydrodynamic drag force on the beads increases with its velocity. The critical frequency is 60 found to be related to the physical dimensions of the beads , [1] where χ is magnetic bead susceptibility, μ o is the permeability of free space, σ o is an experimentally determined parameter representing the effective magnetic moment of the micro-magnets, H ext is the external electromagnet field strength, η is fluid viscosity, and β is the ratio of the bead radius to the distance between adjacent micro-magnets.…”

Section: Introductionmentioning

confidence: 99%

“…The first terms in the SE equations defines efficiency of isolation of the bead of desired susceptibility, while the second term defines the contribution of the undesirable beads. This definition of 15 efficiency also takes into account the loss of any beads that takes place during separation. The calculated SE m and SE l from the flow cytometry measurements were 73.8% and 56.4%, respectively.…”

mentioning

confidence: 99%

“…These results confirm the validity of the simplified one- These results can be used to define the separation efficiency of the FNLM process. To quantitatively analyse the FNLM performance we defined the separation efficiency of the M beads (SE m ) and L beads (SE l ) to be 15 15 2 2 (1 )…”

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confidence: 99%

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Flow enhanced non-linear magnetophoretic separation of beads based on magnetic susceptibility

Kilinc

Ran

et al. 2013

Lab Chip

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Magnetic separation provides a rapid and efficient means of isolating biomaterials from complex mixtures based on their adsorption on superparamagnetic (SPM) beads. Flow enhanced non-linear magnetophoresis (FNLM) is a high-resolution mode of separation in which hydrodynamic and magnetic fields are controlled with micron resolution to isolate SPM beads with specific physical properties. In this article we demonstrate that a change in the critical frequency of FNLM can be used to identify beads with 10 magnetic susceptibilities between 0.01 and 1.0 with a sensitivity of 0.01 Hz -1 . We derived an analytical expression for the critical frequency that explicitly incorporates the magnetic and non-magnetic composition of a complex to be separated. This expression was then applied to two cases involving the detection and separation of biological targets. This study defines the operating principles of FNLM and highlights the potential for using this technique for multiplexing diagnostic assays and isolating rare cell 15 types.

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Section: Resultsmentioning

confidence: 99%

Section: Introductionmentioning

confidence: 99%

Section: Introductionmentioning

confidence: 99%

mentioning

confidence: 99%

mentioning

confidence: 99%

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Flow enhanced non-linear magnetophoretic separation of beads based on magnetic susceptibility

Kilinc

Ran

et al. 2013

Lab Chip

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Two‐Dimensional Programmable Manipulation of Magnetic Nanoparticles on‐Chip

Anandakumar

Torti

Donolato³

et al. 2014

Advanced Materials

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A novel device is designed for on-chip selective trap and two-dimensional remote manipulation of single and multiple fluid-borne magnetic particles using field controlled magnetic domain walls in circular nanostructures. The combination of different ring-shaped nanostructures and field sequences allows for remote manipulation of magnetic particles with high-precision along any arbitrary pathway on a chip surface.

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A Trisymmetric Magnetic Microchip Surface for Free and Two‐Way Directional Movement of Magnetic Microbeads

Sajjad

Lage

McCord

2018

Adv Materials Inter

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it is dominated by the out-of-plane (z) stray component of the magnetic stray field gradient, denoted as dH stray,z /dz, occurring above the magnetised magnetic surface. By alteration of the external magnetic field strength and direction, the occurrence and the position of magnetic field gradients can be varied in a controlled way.Thus, using rotating magnetic fields, the manipulation and transportation of mere and labeled microbeads are realized along defined paths of discrete soft-magnetic patterns. [5][6][7][8][9][10] Similarly, patterned disk arrays are used for the movement of micro particles, [11][12][13][14][15] involving the applications of in-plane and out-of-plane magnetic field sequences. The precise positioning through predefined magnetic tracks [16][17][18][19][20][21] and 2D periodic structures [22] was realized. Moreover, the linear motion of magnetic particles within magnetic tubes [23] was shown. The sorting of microbeads populations in a fixed direction and along a magnetic track on a chip was demonstrated. [24][25][26] In all the latter cases, the particle motion takes place along a predefined axis or direction. A node structure or a switch is necessary in such structures. Limitations in efficiency of movement is another factor that affects the adaptability of magnetic chip based approaches. [27] Overall, using magnetically labeled superparamagnetic beads for selective and free or flexible lateral cell moving together with sorting has not been realized for microfluidic lab-on-chip applications.In this paper, we demonstrate a novel approach for the realization of free lateral and selective motion and, thus, sorting of different ensembles of microbeads by utilizing a broken symmetry in stray field gradients due to symmetrically and asymmetrically applied external magnetic fields. Temporally and spatially tuning of the stray field gradients at corners and along edges in periodically arranged soft-magnetic micromagnets facilitates the individual movement of dissimilar microbead populations. By applying square wave modulated externally applied magnetic field components, we realize the guided motion of microbeads along selectable microcorridors across arrays of magnetic elements. Choosing the proper external magnetic field parameters, we achieve bidirectional propulsion and separation, depending on the size of the microbeads. The areas with 2D periodic structures enable multidirectional and microbead selective transportation of mixed-sized microbead ensembles at a single bead level. The presented dynamical approach of selective bead motion and bead separation paves a new way for the development of alternative magnetic field configurable micromagnetic lab-on-chip devices.The guidance of labeled microcarriers in microfluidic environments is a prerequisite to the development of magnetic pattern assisted lab-on-chip technology. Square wave modulations of in-plane applied magnetic fields enable the forward and backward locomotion of superparamagnetic microspheres on discrete hexagonally arranged ferromagn...

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Microstripes for transport and separation of magnetic particles

Cited by 26 publications

References 16 publications

Flow enhanced non-linear magnetophoretic separation of beads based on magnetic susceptibility

Flow enhanced non-linear magnetophoretic separation of beads based on magnetic susceptibility

Two‐Dimensional Programmable Manipulation of Magnetic Nanoparticles on‐Chip

A Trisymmetric Magnetic Microchip Surface for Free and Two‐Way Directional Movement of Magnetic Microbeads

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