Microsporogenesis, Megasporogensis and Male and Female Gametophyte Development in Feijoa sellowiana (Myrtaceae)

Abstract

Feijoa sellowiana Berg is cultivated as a non-timber forest tree in New Zealand, Colombia and France, and is well known for its high dietary value fruit. F. sellowiana was introduced into China during the 1980s. However, it is particularly prone to erratic fruit set and very little work has been conducted on its reproductive biology. F. sellowiana 'Mammoth' cultivar was subjected to a light microscopy analysis to clarify male and female gametogenesis. The results showed that the formation of anther wall conforms to the basic type. The tapetum is of the glandular type. Cytokinesis in microsporocyte meiosis is of simultaneous type, and the micropore tetrads are tetrahedral. Mature pollen grains are two-celled with three germ pores. The ovules are anatropous, bitegminous, and tenuinucellate. The megaspore tetrads are arranged linearly, and the megaspore at the chalazal end is functional. The functional megaspore undergoes three successive mitoses resulting in the formation of an 8-nucleate embryo sac of the Polygonum type. Our results elucidate the mechanism that regulates sexual reproduction in F. sellowiana, thus expanding the prospects for F. sellowiana breeding programs and further molecular and genetic analyses of this species.

Morphological and Cytological Characterization of Petaloid-type Cytoplasmic Male Sterility in Camellia oleifera

“…The samples were sectioned into 8-to 10-mm-thick slices with a microtome (Leica RM2265; Wetzlar, Germany), stained with hematoxylin-eosin or safraninfast green, and sealed with Canadian neutral resin. Typical sections were observed and photographed using an Olympus BX-51 microscope (Zou et al, 2016). Another set of materials was used for observations of microspore morphology by transmission electron microscopy (TEM).…”

Flower Bud Differentiation and Development of ‘Jinsi No.4’ Jujube (Ziziphus jujuba Mill.) in Hunan Province of Southern China

“…The other ten of the samples were fixed in FAA solution (5% formalin, 5% acetic acid, 90% alcohol, by volume), and stored at 4 °C [15]. Paraffin sectioning followed the methods introduced by Feng Zou et al [16], dehydrated through an ethyl-alcohol series, embedded in paraffin with a 58-60 °C melting point, and sectioned at a thickness of 10 µm by the microtome. The sections were stained with hematoxylin-eosin Y.…”

Flower, seed, and fruit development in three Tunisian species of Polygonum: Implications for their taxonomy and evolution of distyly in Polygonaceae

“…[39]. The mature anther wall of P. equisetiforme and P. maritimum is characterized by well-developed endothecium also prevalent in many dicotyledonous species such as Onobrychis schahuensis Bornm [40], Acca sellowiana (O. Berg) Burret [41], and Camellia japonica L. [42]. On the contrary, recent studies have shown that the endothelium is not differentiated and or absent in the anther of Camellia yunnanensis var.…”