Microspore wall organisation and ultrastructure in two species of<i>Selaginella</i>(Lycophyta) producing permanent tetrads

Morbelli, Marta A.; Lugardon, Bernard

doi:10.1080/00173134.2012.691109

Cited by 8 publications

(13 citation statements)

References 6 publications

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“…It is used since the nineties for plant cuticles, mainly for fossils, summarized in all these former papers. As unfortunately the detailed method appears only partially in Lugardon’s thesis [2] and in spore – megaspore – pollen articles [3] , [4] , [5] , [6] , [7] , beside of the English text of the present paper, we reproduced here the most interesting method’s page hand-written in French by Lugardon with a pencil ( Fig. 1 ), during one of my first stays in Toulouse, also partly hand-written by me for some procedure details.…”

Section: Methods Detailsmentioning

confidence: 99%

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Method for ultrastructural fine details of plant cuticles by transmission electron microscopy

Guignard

2021

MethodsX

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This method for plant cuticles was adapted from spores and pollen procedure. It already enabled to prepare ultrathin plant cuticle sections of 60–70 nanometers of thickness, in high numbers as a routine, and allowed statistical measurements as any other macro- and/or micro-features. It provides through transmission electron microscope clean and contrasted observations of layers and sublayers of the cuticle, at a magnification over 100,000 times. It has been used efficiently for taxonomy, signature of environment and evolution topics since the end of the nineties (Gaëtan Guignard, 2019), however it is detailed here step by step for the first time, and illustrated. It can serve in the future for many purposes, like other taxonomical comparisons, palaeoreconstructions and evolutionary considerations. Value of the protocol This method provides ultrathin plant cuticle sections of 60–70 nanometers of thickness Details of layers and sublayers of the cuticle are easily observed High numbers of sections allows statistical measurements as a routine

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Section: Methods Detailsmentioning

confidence: 99%

“…https://doi-org.inee.bib.cnrs.fr/10.1016/j.revpalbo.2009.03.003 . [7] M.A. Morbelli, B. Lugardon, Microspore wall organisation and ultrastructure in two species of Selaginella (Lycophyta) producing permanent tetrads, Grana 51 (2012) 97–106.…”

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confidence: 99%

Method for ultrastructural fine details of plant cuticles by transmission electron microscopy

Guignard

2021

MethodsX

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“…Spring, and S. lepidophylla (Hook & Grev.) Spring (Morbelli and Lugardon, 2012), whose microspores remain attached in tetrads. For instance, the spore tetrad of S. stenophylla has a diameter of 41.6 ± 2.16 µm and a higher Vt of 14.96 ± 1.08 cm·s −1 ( n = 31) than do the individual microspores of S. pallescens (C. Presl) Spring, which are 26 × 30 µm and have a slower Vt of 9.9 ± 0.92 cm·s −1 ( n = 31).…”

Section: Discussionmentioning

confidence: 99%

Terminal velocity of fern and lycopod spores is affected more by mass and ornamentation than by size

Gómez‐Noguez

Domínguez‐Ugalde

Flores‐Galván

et al. 2022

American J of Botany

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Premise Terminal velocity (Vt) is an important factor for the dispersal of biological particles but has scarcely been studied for anemochorous fern spores, and the influence of spore characteristics on Vt has not been evaluated. Here, we measured the Vt of 1234 spores of 18 fern species and two Selaginella microspores using videoimaging analysis and evaluated the effects of mass, size, and ornamentation on Vt. Methods We designed a sedimentation tower with a graduated microtelescope attached to a high‐speed video camera to record falling particles and measure the Vt of fern spores using video‐image processing software. Spores were measured for each species and their size correlated with Vt. Results The Vt of fern spores ranged from 4.7 cm·s−1 (Cyathea costaricensis) to 18.85 cm·s−1 (Acrostichum danaeifolium). The method is accurate and reliable as predicted by Stokes model for glass beads of known density and size. In addition, Vt had a higher correlation coefficient with mass (ρ = 0.72) than size (ρ = 0.20), and ornamental appendages reduced Vt. Conclusions The reported values of Vt of fern spores are within the range of different biological airborne particles such as moss spores and pollen grains of seed plants. The results showed that spore ornamentation is directly related to Vt rather than spore size and may increase or decrease the drag. This method will aid future aerobiological research on biological particles.

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“…Among the extant species of Selaginella, the common pattern is that microspores are disseminated indi- vidually (as monads), but they may also remain in tetrads after their release due to different connections or fusions of the spore wall (Morbelli & al. 2001;Tryon & Lugardon 2001;Morbelli & Lugardon 2012;Zhou & al. 2015a;.…”

Section: Selaginella Wangboimentioning

confidence: 99%

Selaginella in Cretaceous amber from Myanmar

et al. 2022

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Selaginella (Selaginellales, Selaginellaceae) is the most speciose genus of lycophytes and, with approximately 750 recognized present-day species, also one of the largest genera of vascular plants. However, the evolutionary history of this species richness remains largely unresolved. Recent research suggests that Selaginella was diverse already in the mid-Cretaceous and shows that S. subg. Stachygynandrum dates back at least to the incipient Angiosperm Terrestrial Revolution some 100 million years ago. Here, we describe 20 new fossil-species of Selaginella based on fertile shoots and spores preserved in mid-Cretaceous Kachin amber from Myanmar and emend the previously described S. cretacea. Ten of the species (S.

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Microspore wall organisation and ultrastructure in two species ofSelaginella(Lycophyta) producing permanent tetrads

Cited by 8 publications

References 6 publications

Method for ultrastructural fine details of plant cuticles by transmission electron microscopy

Method for ultrastructural fine details of plant cuticles by transmission electron microscopy

Terminal velocity of fern and lycopod spores is affected more by mass and ornamentation than by size

Selaginella in Cretaceous amber from Myanmar

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