Microspore embryogenesis in Brassica: calcium signaling, epigenetic modification, and programmed cell death

Ahmadi, Behzad; Ahmadi, Medya; Silva, Jaime A. Teixeira da

doi:10.1007/s00425-018-2996-5

Cited by 11 publications

(4 citation statements)

References 86 publications

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“…Somatic regeneration from flower buds and second cycle gynogenesis offers additional opportunities to obtain diploids from mixoploid and tetraploid lines. In addition, the use of more effective doubling agents such as nitrous oxide gas (Molenaar et al 2018), n-butanol (Ahmadi et al 2018) and pronamide (Klutschewaski et al…”

Section: Conclusion and Prospectsmentioning

confidence: 99%

Doubled haploid production in onion (Allium cepa L.): from gynogenesis to chromosome doubling

Khan

Vijayalakshmi

Raja

et al. 2020

Plant Cell Tiss Organ Cult

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Bulb onion ( Allium cepa L.) is an allogamous diploid (2n = 16) important for its culinary uses, nutritional value, and medicinal benefits. Despite its economic importance, onion yields and bulb quality are declining, emphasizing the need for new and improved strategies for maintaining and enhancing overall crop quality. Development of inbred lines in onion through traditional breeding is often difficult due to its biennial life cycle, inbreeding depression, and comparatively high heterozygosities. Moreover, genetic research in onion has been hampered by large nuclear genome size. In this regard, gynogenic doubled haploids promise several advantages over inbred lines in support of onion breeding programs and genetic studies. These include complete homozygosity in doubled haploid lines, reduced DNA methylation, elimination of deleterious alleles, and amenability to genetic analysis. This review focuses on the application of in vitro gynogenesis for producing doubled haploids in onion. Factors influencing haploid induction, methods for inducing chromosome doubling and ploidy assessment, evaluation of haploid progenies and doubled haploid lines, and features of doubled haploids potentially useful in crop improvement and genetic studies, are discussed. We identify four major limitations to the success and efficiency of in vitro gynogenesis in onion and discuss strategies for mitigating the negative impacts they pose. This review may be useful to research programs producing doubled haploids in onion or other Allium species using in vitro gynogenesis.

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Section: Conclusion and Prospectsmentioning

confidence: 99%

Doubled haploid production in onion (Allium cepa L.): from gynogenesis to chromosome doubling

Khan

Vijayalakshmi

Raja

et al. 2020

Plant Cell Tiss Organ Cult

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“…For some species, isolated microspore culture protocols have been well-established and are routinely used in laboratories for developing new varieties as well as for basic research areas, such as genomics, gene expression, and genetic mapping (Ferrie and Caswell, 2011). The last few years have provided ample evidence that has allowed researchers of Brassica to markedly increase their understanding of the molecular and subcellular changes underlying the early events of totipotency that permit microspores to develop into embryos and facilitate their transformation (Ahmadi et al, 2018). Isolated plant microspores can be diverted from their normal gametophytic pathway toward sporophytic development and used also in stress-responsive studies.…”

Section: Introductionmentioning

confidence: 99%

The Resistance of Oilseed Rape Microspore-Derived Embryos to Osmotic Stress Is Associated With the Accumulation of Energy Metabolism Proteins, Redox Homeostasis, Higher Abscisic Acid, and Cytokinin Contents

et al. 2021

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The present study aims to investigate the response of rapeseed microspore-derived embryos (MDE) to osmotic stress at the proteome level. The PEG-induced osmotic stress was studied in the cotyledonary stage of MDE of two genotypes: Cadeli (D) and Viking (V), previously reported to exhibit contrasting leaf proteome responses under drought. Two-dimensional difference gel electrophoresis (2D-DIGE) revealed 156 representative protein spots that have been selected for MALDI-TOF/TOF analysis. Sixty-three proteins have been successfully identified and divided into eight functional groups. Data are available via ProteomeXchange with identifier PXD024552. Eight selected protein accumulation trends were compared with real-time quantitative PCR (RT-qPCR). Biomass accumulation in treated D was significantly higher (3-fold) than in V, which indicates D is resistant to osmotic stress. Cultivar D displayed resistance strategy by the accumulation of proteins in energy metabolism, redox homeostasis, protein destination, and signaling functional groups, high ABA, and active cytokinins (CKs) contents. In contrast, the V protein profile displayed high requirements of energy and nutrients with a significant number of stress-related proteins and cell structure changes accompanied by quick downregulation of active CKs, as well as salicylic and jasmonic acids. Genes that were suitable for gene-targeting showed significantly higher expression in treated samples and were identified as phospholipase D alpha, peroxiredoxin antioxidant, and lactoylglutathione lyase. The MDE proteome profile has been compared with the leaf proteome evaluated in our previous study. Different mechanisms to cope with osmotic stress were revealed between the genotypes studied. This proteomic study is the first step to validate MDE as a suitable model for follow-up research on the characterization of new crossings and can be used for preselection of resistant genotypes.

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“…More importantly, DNA methylation also plays an important role in SE development. Different SE developmental stages are companied by different DNA methylation levels in plants, such as Bactris gasipaes Kunth, Brassica, and rice [23][24][25].…”

Section: Introductionmentioning

confidence: 99%

Effects of Medium Supplements on Somatic Embryo Maturation and DNA Methylation in Pseudotsuga gaussenii Flous, a Species under Protection

Gao

Chen

Cui

et al. 2022

Forests

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Pseudotsuga gaussenii is a forest species under protection in China. Propagation via somatic embryogenesis is efficient and needed for recovering this species. In this research, effects of medium supplements, i.e., methylglyoxal-bis (guanylhydrazone) (MGBG), polyethylene glycol (PEG), and maltose, on somatic embryo (SE) development were studied. More mature SEs developed with the pre-maturation treatment medium supplemented with 50 μM or 100 μM MGBG. During SE maturation, when the medium was supplemented with both PEG and maltose, the over-proliferation of embryogenic tissue was suppressed. When maltose was eliminated from the maturation medium, SEs were slightly smaller but developed better than other treatments due to the supply of lower carbon source, whereas when PEG was taken out from the maturation medium, no mature SE was obtained, only the heavily-proliferated plant tissue. Meanwhile, DNA methylation levels differed in these SE maturation cultures at the late culture stage of SE maturation. The level increased sharply with no maltose treatment (20.28%) while decreasing without PEG supplement (10.35%). DNA methyltransferase genes MET1-1, MET1-2, MET1-3, CMT3, DRM1, and DRM2 were partially cloned in this study to detect their expression level via qPCR. Expression of these genes, except MET1-3, in the culture of no PEG was higher significantly than the cultures of other treatments at weeks 1, 3, and 10. Our results suggested that MGBG and/or PEG play an important role in stimulating SE development and maturation. Furthermore, sugar supplements at a lower level benefited SE maturation in Pseudotsuga gaussenii.

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Microspore embryogenesis in Brassica: calcium signaling, epigenetic modification, and programmed cell death

Cited by 11 publications

References 86 publications

Doubled haploid production in onion (Allium cepa L.): from gynogenesis to chromosome doubling

Doubled haploid production in onion (Allium cepa L.): from gynogenesis to chromosome doubling

The Resistance of Oilseed Rape Microspore-Derived Embryos to Osmotic Stress Is Associated With the Accumulation of Energy Metabolism Proteins, Redox Homeostasis, Higher Abscisic Acid, and Cytokinin Contents

Effects of Medium Supplements on Somatic Embryo Maturation and DNA Methylation in Pseudotsuga gaussenii Flous, a Species under Protection

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