Microspore development during in vitro androgenesis in triticale

González, Juan M.; Jouve, N.

doi:10.1007/s10535-005-3028-4

Cited by 19 publications

(19 citation statements)

References 29 publications

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“…Features they regarded as phenomena connected to switching of the developmental pathway were also observed during our study: cell enlargement, vacuole fragmentation, enrichment of the cell lumen with cytoplasm, mitotic divisions of a frequency and symmetry different from that typical for pollen development, and the emergence and later polarization of granular structures (ER, starch grains). In contrast to other reports (Sunderland, 1973;Gonzáles and Jouve, 2005), we relatively often observed the first symmetric mitotic division in the vicinity of the sporoderm, but it was not clear whether those microspores would develop further into multinuclear or multicellular structures. During microspore culture of triticale, the first mitotic division of microspores is reported to be usually symmetric and followed by the formation of two vegetative-like nuclei or cells of similar size (Gonzáles and Jouve, 2005).…”

Section: Discussioncontrasting

confidence: 99%

“…In contrast to other reports (Sunderland, 1973;Gonzáles and Jouve, 2005), we relatively often observed the first symmetric mitotic division in the vicinity of the sporoderm, but it was not clear whether those microspores would develop further into multinuclear or multicellular structures. During microspore culture of triticale, the first mitotic division of microspores is reported to be usually symmetric and followed by the formation of two vegetative-like nuclei or cells of similar size (Gonzáles and Jouve, 2005).…”

Section: Discussioncontrasting

confidence: 99%

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Cell Structural Reorganization During Induction of Androgenesis in Isolated Microspore Cultures of Triticale (xTriticosecale Wittm.)

Dubas¹,

Wędzony²,

Petrovská³

et al. 2010

Acta Biologica Cracoviensia Series Botanica

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Upon stress treatment, isolated microspores of triticale (×Triticosecale Wittm.) were directed towards sporophytic development (androgenesis). We used fluorescence microscopy to study the cell structural reorganization associated with the process. Changes in the developmental pathway coincided with the character of the microtubular cytoskeleton configuration, the number and direction of nuclear divisions, changes in vacuolization, the distribution of mitochondria, ER and starch grains, and the architecture of new cell wall formation. A band of diffused fluorescence surrounding the nucleus was observed before the first symmetric division of microspores. This structure most likely represents a preprophase band (PPB). Successive mitotic divisions within the microspore wall led to the formation of multinucleate or multicellular structures consisting of one or two domains of cells differing in size. They were later released from the sporoderm and continued further development with features typical for a monocotyledonous embryo. The pattern of internal architecture of androgenic structures depended on their developmental phase. Before and after release from the microspore wall, cortical microtubules (MTs) exhibited various configurations without preferential orientation. They formed a denser network in the region opposite to the sporoderm rupture site. Released multicellular structures showed both intensely fluorescing cortical MTs and more dispersed endoplasmic MTs radiating along the cytoplasmic strands from the nuclear region to the cell cortex. Up to globular stage, isotropically expanding cells of androgenic embryos showed a random pattern of MTs. This is the first report that successive events of androgenic development of triticale microspores are associated with MT reorganization. The results support the view that changes in cytoskeleton architecture are critical during induction of androgenesis.K Ke ey y w wo or rd ds s: : Androgenesis, microspores, triticale, cytoskeleton, microtubules.

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Section: Discussioncontrasting

confidence: 99%

Section: Discussioncontrasting

confidence: 99%

Cell Structural Reorganization During Induction of Androgenesis in Isolated Microspore Cultures of Triticale (xTriticosecale Wittm.)

Dubas¹,

Wędzony²,

Petrovská³

et al. 2010

Acta Biologica Cracoviensia Series Botanica

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“…Sporophytic structures composed of generative-like and vegetativelike nuclei can be observed occasionally (Fan et al, 1988;Reynolds, 1993;González-Melendi et al, 1996;Kaltchuk-Santos et al, 1997;González and Jouve, 2005), but it is not known whether sustained division of generative-like cells contributes to the formation of viable embryos. Our results show that the LEC1 embryo reporters are expressed only in the microspore and vegetative cell after heat stress treatment, while exposure to heat stress and TSA also induces LEC1 expression in the generative cell.…”

Section: Acquisition Of Embryo Identitymentioning

confidence: 99%

The Histone Deacetylase Inhibitor Trichostatin A Promotes Totipotency in the Male Gametophyte

et al. 2014

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The haploid male gametophyte, the pollen grain, is a terminally differentiated structure whose function ends at fertilization. Plant breeding and propagation widely use haploid embryo production from in vitro-cultured male gametophytes, but this technique remains poorly understood at the mechanistic level. Here, we show that histone deacetylases (HDACs) regulate the switch to haploid embryogenesis. Blocking HDAC activity with trichostatin A (TSA) in cultured male gametophytes of Brassica napus leads to a large increase in the proportion of cells that switch from pollen to embryogenic growth. Embryogenic growth is enhanced by, but not dependent on, the high-temperature stress that is normally used to induce haploid embryogenesis in B. napus. The male gametophyte of Arabidopsis thaliana, which is recalcitrant to haploid embryo development in culture, also forms embryogenic cell clusters after TSA treatment. Genetic analysis suggests that the HDAC protein HDA17 plays a role in this process. TSA treatment of male gametophytes is associated with the hyperacetylation of histones H3 and H4. We propose that the totipotency of the male gametophyte is kept in check by an HDAC-dependent mechanism and that the stress treatments used to induce haploid embryo development in culture impinge on this HDAC-dependent pathway.

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“…Several abiotic stresses, alone or in combination, are used to trigger ME. These include cold shock (Gonzalez and Jouve 2005;Labbani et al 2005), heat-shock (Custers et al 1994;Touraev et al 1996;Rimberia et al 2005), osmotic shock (Caredda et al 2000;Touraev et al 2001;Li and Devaux 2003;Wojnarowiez et al 2004), pH variation (Barinova et al 2004), nutrient starvation (Touraev et al 1996), exposure to heavy metals, such as lithium (Zonia and Tupy 1995) or copper sulphate (Wojnarowiez et al 2002). In barley anthers, ME is induced using a combined coldand osmotic shock treatment (Caredda et al 2000).…”

Section: Introductionmentioning

confidence: 99%

Microspore embryogenesis in barley: anther pre-treatment stimulates plant defence gene expression

Jacquard

Mazeyrat-Gourbeyre

Devaux³

et al. 2008

Planta

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Microspore embryogenesis (ME) is a process in which the gametophytic pollen programme of the microspore is reorientated towards a new embryo sporophytic programme. This process requires a stress treatment, usually performed in the anther or isolated microspores for several days. Despite the universal use of stress to induce ME, very few studies have addressed the physiological processes that occur in the anther during this step. To further understand the processes triggered by stress treatment, we followed the response of anthers by measuring the expression of stressrelated genes in two barley (Hordeum vulgare L.) cultivars differing in their ME response. Genes encoding enzymes involved in oxidative stress (glutathione-S-transferase, GST; oxalate oxidase, OxO), in the synthesis of jasmonic acid (13-lipoxygenase, Lox; allene oxide cyclase, AOC; allene oxide synthase, AOS) and in the phenylpropanoid pathway (phenylalanine ammonia lyase, PAL), as well as those encoding PR proteins (Barwin, chitinase 2b, Chit 2b; glucanase, Gluc; basic pathogenesis-related protein 1, PR1; pathogenesis-related protein 10, PR10) were up-regulated in whole anthers upon stress treatment, indicating that anther perceives stress and reacts by triggering general plant defence mechanisms. In particular, both OxO and Chit 2b genes are good markers of anther reactivity owing to their high level of induction during the stress treatment. The effect of copper sulphate appeared to limit the expression of defence-related genes, which may be correlated with its positive effect on the yield of microspore embryos.

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Microspore development during in vitro androgenesis in triticale

Cited by 19 publications

References 29 publications

Cell Structural Reorganization During Induction of Androgenesis in Isolated Microspore Cultures of Triticale (xTriticosecale Wittm.)

Cell Structural Reorganization During Induction of Androgenesis in Isolated Microspore Cultures of Triticale (xTriticosecale Wittm.)

The Histone Deacetylase Inhibitor Trichostatin A Promotes Totipotency in the Male Gametophyte

Microspore embryogenesis in barley: anther pre-treatment stimulates plant defence gene expression

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