Microsphere sedimentation arrays for multiplexed bioanalytics

Moser, Christoph; Mayr, Torsten; Klimant, Ingo

doi:10.1016/j.aca.2005.11.066

Cited by 22 publications

(20 citation statements)

References 27 publications

Supporting

Mentioning

Contrasting

Order By: Relevance

“…The magnetic nature of the particles allows easy manipulation or separation because they are attracted or repelled by a magnet. 12 The trapping of MagNPs in a desired location facilitates the sophisticated design of analysis schemes; e.g., rinsing after a reaction or consecutive reactions with different reagents is easily performed for the entrapped particles. Other physical fields, including electric, 13 dielectric, 14,15 flow, [16][17][18] optical, 17 and acoustic fields, [19][20][21] have also been employed for particle manipulation and separation.…”

Section: Introductionmentioning

confidence: 99%

Acoustic Sensing Based on Density Shift of Microspheres by Surface Binding of Gold Nanoparticles

et al. 2017

View full text Add to dashboard Cite

Herein, we propose a concept for sensing based on density changes of microparticles (MPs) caused by a biochemical reaction. The MPs are levitated by a combined acoustic-gravitational force at a position determined by the density and compressibility. Importantly, the levitation is independent of the MPs sizes. When gold nanoparticles (AuNPs) are bound on the surface of polymer MPs through a reaction, the density of the MPs dramatically increases, and their levitation position in the acoustic-gravitational field is lowered. Because the shift of the levitation position is proportional to the number of AuNPs bound on one MP, we can determine the number of molecules involved in the reaction. The avidinbiotin reaction is used to demonstrate the effectiveness of this concept. The number of molecules involved in the reaction is very small because the reaction space is small for an MP; thus, the method has potential for highly sensitive detection.

show abstract

Section: Introductionmentioning

confidence: 99%

Acoustic Sensing Based on Density Shift of Microspheres by Surface Binding of Gold Nanoparticles

et al. 2017

View full text Add to dashboard Cite

show abstract

“…We applied luminescence lifetime imaging to decode randomly ordered bead arrays. The luminescence decay of the encoded beads was adjusted by staining beads with a combination of Tris(4,7-diphenyl-1,10-phenanthroline)ruthenium(II) chloride and crystal violet as the fluorescence resonance energy acceptor (Moser et al ., 2006). Thus, beads can be clearly distinguished and attributed to a bead class (Fig.…”

Section: Resultsmentioning

confidence: 99%

“…Magnetic polystyrene microspheres of 6-µ m diameter were purchased from Micromod (Rostock, Germany) and stained with Pt(II) meso-tetra(pentafluorophenyl)porphine showing excitation and emission maxima of 404 and 652 nm, respectively. To obtain specimens showing the selected fluorescence lifetime, magnetic polystyrene microspheres were stained with combinations of Tris(4,7-diphenyl-1,10-phenanthroline)ruthenium(II) chloride and crystal violet as acceptor dye (Moser et al ., 2006). Optical filters from Zeiss, Omega Optical (Brattleboro, VT, USA) and Schott (Mainz, Germany) were used.…”

Section: Specimenmentioning

confidence: 99%

Filter cubes with built‐in ultrabright light‐emitting diodes as exchangeable excitation light sources in fluorescence microscopy

Moser

Mayr

Klimant

2006

Journal of Microscopy

View full text Add to dashboard Cite

SummaryThe use of ultrabright light-emitting diodes as a potential substitute for conventional excitation light sources in fluorescence microscopy is demonstrated. We integrated ultrabright light-emitting diodes in the filter block of a conventional fluorescence microscope together with a collimating Fresnel lens, a holographic diffuser and emission filters. This setup enabled convenient changes between different excitation light sources and resulted in high excitation efficiencies. Quantitative comparison of image intensities of test samples revealed that light-emitting diodes yielded intensities in the range of a mercury arc lamp depending on the wavelength. The use of ultrabright light-emitting diodes also enabled luminescence lifetime imaging without the need for image intensification.

show abstract

“…This procedure is mostly used for the modification of commercially available magnetic particles [27,38,156,157].…”

Section: Organic Polymersmentioning

confidence: 99%

“…A spectral encoding scheme for multiplexed sedimentation arrays was accomplished via swelling of polymeric magnetic particles from Micromod GmbH [156,157]. It is important to mention that this route can only be successful for matrices fulfilling certain requirements.…”

Section: Modif Ication Of Non-luminescent Magnetic Particlesmentioning

confidence: 99%

Luminescent magnetic particles: structures, syntheses, multimodal imaging, and analytical applications

Mistlberger

Klimant

2010

Bioanal Rev

Self Cite

View full text Add to dashboard Cite

Luminescent magnetic particles (LuMaPs) are attractive tools for life science applications such as multimodal imaging, analyte monitoring, nanotherapeutics, and combinations thereof. LuMaPs consist of at least one magnetic and one luminescent component which often are incorporated in a (polymeric) matrix. A large variety of materials do exist for the components that make up LuMaPs. However, a smart selection and combination is required for achieving useful tools. While the magnetic component mainly influences the response to a magnetic field, the luminophore can act as label, sensor, or therapeutic agent. The matrix fulfills tasks such as stabilizing the luminophore and magnet, carrying useful functional groups on its surface, or hosting smart drug delivery systems. Surface modifications with targeting ligands can further improve the applicability of LuMaPs, for example in biomedicine. This review provides an overview on LuMaPs with respect to the materials used and to its structures. Routes toward LuMaPs are outlined, and potential applications are discussed.

show abstract

Microsphere sedimentation arrays for multiplexed bioanalytics

Cited by 22 publications

References 27 publications

Acoustic Sensing Based on Density Shift of Microspheres by Surface Binding of Gold Nanoparticles

Acoustic Sensing Based on Density Shift of Microspheres by Surface Binding of Gold Nanoparticles

Filter cubes with built‐in ultrabright light‐emitting diodes as exchangeable excitation light sources in fluorescence microscopy

Luminescent magnetic particles: structures, syntheses, multimodal imaging, and analytical applications

Contact Info

Product

Resources

About