Microsphere-assisted enhanced photothermal lens detection integrated with digital holographic microscopy for 3D particle sensing and thermal diffusivity measurement

Abstract

In this paper, we show that by extending the concept of “microsphere-assisted imaging” into thermal lens (TL) detection, enhancement of photothermal detection is achieved. Furthermore, by integration of TL detection and digital holographic microscopy (DHM), we provide simultaneous measurement of photothermal properties as well as complementary imaging of the sample. Simultaneous acquisition of photothermal properties and imaging is essential for characterization of a variety of samples in biochemistry, material science, and process technologies. TL is a sensitive methodology to detect low concentrations of analytes in low-loss samples, and DHM in the transmission mode is an effective technique for label-free, non-contact, and real-time imaging and measurement of phase objects, such as biomaterials. We demonstrate the usefulness of the TL-DHM integrated system by applying it for acquisition of photothermal maps of samples as well as obtaining their morphometrical information. Additionally, we measure the thermal diffusivity of polydimethylsiloxane because of the focusing effect of polystyrene microspheres. The system has the potential to be applied for a variety of objects including bio-samples and may be proposed as a bench-top characterization device.