Microspectrophotometric determination of the haemoglobin oxygen saturation in haemolyzed arterialized capillary blood

Abstract

A description is given of a rapid spectrophotometric micromethod of determining the oxygen saturation in arterialized capillary blood. During sampling, the blood was haemolyzed in a glass capillary with the aid of the haemolytic agent Sterox SE.Extinctions were measured at 795 nm and 655 nm in a cuvette made up of slides (d = I mm). A single determination is sufficiently exact. Capillary blood from the arterialized earlobe was found to be an adequate substitute for blood from an artery. This method showed good agreement with the haemoreflector method.

The Measurement of Oxygen Saturation and Po2

“…Summary, Graphical representation of the results shows an even scatter about the line of equality in all but the methods of Maas et al, (1964) and Tsao et al, (1955) where the results are consistently low. Determination of the constants used in the formula and nomogram respectively for these methods gave values slightly different from those of the authors and recalculation of the saturation values gave a better correlation with the reference method.…”

“…glass Hemolysis is generally achieved by the addition of a minimal quantity of saponin solution to the blood. Other methods have been described, for example Deibler (1959) used Triton X-I00 and Maas et al, (1964) the detergent Sterox. Anderson et al, (1962) centrifuged blood in capillary tubes and lysed the red cells by alternate freezing and thawing.…”

Some Aspects of the Oxygen Transport in Patients with Chronic Obstructive Lung Diseases and Respiratory Insufficiency

“…Oxygen pressure (P0;), carbon diox ide pressure (PC0,) and pH were measured with an acid-base-analyzer with a P0, module (Radiometer, Copenhagen, Denmark). Oxygen saturation (S(>,) was measured by a spectrophotometric method [Maas et al, 1964], The ODC was measured with the Dissociation Curve Analyzer (type DCA. Radiome ter, Copenhagen, Denmark) according to Duvelleroy et al [1970], PSu and P5,K -4| were calculated from the ODC.…”

Some Circulatory Aspects of the Oxygen Transport in Patients with Emphysema

“…2 (Netapo, Epalinges, Switzer land). In order to measure partial oxygen pressure (P 0 2), carbon dioxide pressure (PC 02) and pH in blood samples an acid base analyzer with PO: mo dule (Radiometer, Copenhagen, Denmark) was used, while oxygen saturation (S02) values were de termined according to a spectrophotometric method by Maaset al [2].…”