Microsecond-resolved smartphone time-gated luminescence spectroscopy

Deng, Qisheng; Liu, Yulei; Zhu, Zece; Shu, Xuewen

doi:10.1364/ol.467458

Cited by 4 publications

(7 citation statements)

References 46 publications

Supporting

Mentioning

Contrasting

Order By: Relevance

“…By using a time-gated technique, different lifetimes lead to different detected emission intensities, so that fluorescence and phosphorescence with distinct lifetime can be distinguished . To further examine the phosphorescence component of S -Cu 3 , we separately captured time-gated luminescence spectra of S -Cu 3 in the solid state, solution state, and film state (Figure a–c).…”

Section: Resultsmentioning

confidence: 99%

High-Efficiency Pure Blue Circularly Polarized Phosphorescence from Chiral N-Heterocyclic-Carbene-Stabilized Copper(I) Clusters

Ma,

Si,

et al. 2023

J. Am. Chem. Soc.

View full text Add to dashboard Cite

Circularly polarized luminescence (CPL) materials have attracted considerable attention for their promising applications in encryption, chiral sensing, and three-dimensional (3D) displays. However, the preparation of high-efficiency, pure blue CPL materials remains challenging. In this study, we reported an enantiomeric pair of triangle copper(I) clusters (R/S -Cu 3 ) rigidified by employing chiral N-heterocyclic carbene (NHC) ligands with two pyridine-functionalized wingtips. These chiral clusters emitted pure blue phosphorescence that overlapped with that of the commercial blue phosphor having Commission Internationale de l’Eclairage (CIE) chromaticity coordinates of (0.14, 0.10), and the films exhibited an unprecedented photoluminescence quantum yield (PLQY) of ∼70.0%. Additionally, the solutions showed very bright circularly polarized phosphorescence (CPP) with a dissymmetry factor of ±2.1 × 10–3. The excellent solubility and photostability endowed these pure-blue-emitting chiral clusters with promising applications as pure blue CPP inks for 3D printing white objects, such as precise-atomic-enlarged models of metal clusters and a lovely white stereoscopic “rabbit”. The intricate mechanism underlying blue phosphorescence in this small cluster and across various states is elucidated through a comprehensive approach that integrates thorough analysis of luminescence properties, controlled experiments, and theoretical calculations. For the first time, we propose that the dominant high-energy emission center is constituted by delocalized hybrid orbitals over multiple atomic centers, encompassing both the metal and the coordinated atoms. This challenges stereotypical assumptions that the cluster center solely supports low-energy emissions. This work expands the currently limited range of CPP functional materials and provides a new direction for CPP applications involving NHC-stabilized metal clusters.

show abstract

Section: Resultsmentioning

confidence: 99%

High-Efficiency Pure Blue Circularly Polarized Phosphorescence from Chiral N-Heterocyclic-Carbene-Stabilized Copper(I) Clusters

Ma,

Si,

et al. 2023

J. Am. Chem. Soc.

View full text Add to dashboard Cite

show abstract

“…Comparing designs, the single-chopper optical-switch design used by Deng et al , is advantageous in that it only requires one chopper, but does so with much more sophisticated electronics than our double-chopper design (the FPGA board in ref costs several hundred dollars). Moreover, the optical-switch design does not, to our understanding, allow for independent control of excitation pulse duration, delay time, and signal integration time.…”

Section: Discussionmentioning

confidence: 99%

“…At the outset of this research, there were no published examples of a smartphone-based TG imaging device for LLCs. In the intervening time, two reports were published: , a single-chopper design (with a grating and an optical switch to pulse a violet laser) that was used to measure the TG PL spectrum and lifetime of powdered Eu(III) LLC, and a similar design that was used to image a mouse kidney section marked with an Eu(III) LLC . Although valuable proofs of concept for TG imaging, these studies did not image a Tb(III) LLC, did not address analytical utility, and utilized breadboarded setups without real portability.…”

Section: Discussionmentioning

confidence: 99%

“…Despite this utility, the development of TG PL measurements with a smartphone has been limited to a preliminary proof of concept of imaging without analytical benchmarking. 23,24 Here, we present a novel design for a smartphone-based device for analytical TG PL imaging. Using a luminescent terbium(III) complex (LTbC) and nanoparticles doped with a luminescent europium(III) complex (EuNP), the device is validated for TG suppression of background from biologically relevant samples.…”

Section: ■ Introductionmentioning

confidence: 99%

“…Background suppression is potentially ideal for POCDs because it facilitates the use of minimally processed samples. Despite this utility, the development of TG PL measurements with a smartphone has been limited to a preliminary proof of concept of imaging without analytical benchmarking. , …”

Section: Introductionmentioning

confidence: 99%

See 2 more Smart Citations

Quantitative and Multiplexed Chopper-Based Time-Gated Imaging for Bioanalysis on a Smartphone

Kanani,

Tsai,

Algar

2023

Anal. Chem.

View full text Add to dashboard Cite

Smartphones are emerging platforms for point-ofcare diagnostics (POCDs), where the on-board camera is, for example, used to image fluorescence. Many laboratory instruments are capable of time-gated (TG) photoluminescence (PL) measure-ments�an analytical method leveraged by multiple commercial assay kits. When paired with long-lived PL emitters such as luminescent lanthanide complexes (LLCs), time-gating eliminates background from sample autofluorescence and many other sources. This capability is amenable to minimally processed samples and would thus be useful for POCDs on a smartphonebased platform. Here, we report a double-chopper design for TG PL imaging using a portable, 3D-printed, smartphone-based device. The rotation speed, dimensions, and overlap of the chopper blades and gaps set the timing parameters, with delay times on the order of hundreds of microseconds to milliseconds. The device was capable of quantitative TG imaging of PL from terbium(III) and europium(III) LLCs, including rejection of short-lived PL background from serum and tissue phantoms, spectral and temporal multiplexing, a model time-gated Forster resonance energy transfer (TG-FRET) assay, and imaging of cells. As the first smartphone-based demonstrations of these important analytical capabilities, this work is an important foundation for developing POCD methods based on TG PL imaging.

show abstract

Sensitive and High-Throughput Time-Resolved Luminescence Detection of Tetracycline in Milk for Eliminating Background Fluorescence on a Miniaturized Apparatus

Luo,

Zhu

2024

Anal. Chem.

Self Cite

View full text Add to dashboard Cite

Fluorescence detection has always suffered from high background fluorescence from real samples such as milk. Therefore, cumbersome pretreatments of samples were necessary to remove the fluorescent substances but led to long processing times and low efficiency. Time-resolved luminescence detection is a powerful technique for eliminating short-lived background fluorescence without additional pretreatments. However, the related instruments are usually equipped with high-speed excitation sources and detectors, which are always bulky and expensive. Herein, we developed a low-cost and miniaturized imaging system for high-throughput time-gated luminescence detection. An UV LED array was used to excite multiple samples, the luminescence of which could be detected by a smartphone simultaneously. An analog circuit was designed to synchronize the LED to the mechanical chopper to eliminate the background signals resulting from scattering and short-lived autofluorescence. Compared to other synchronous circuits based on FPGAs and microcontrollers, this analog circuit required no programming and memory. For the first time, high-throughput time-resolved luminescence detection of tetracycline in milk without any separation or enrichment was achieved by utilizing a smartphone as a camera, and the scattered signals and the background fluorescence were eliminated efficiently. The limit of detection reached as low as 53 nM (∼0.024 ppm), lower than the residue limit set by the European Union. This high-throughput time-gated luminescence detection method can be used for quantitative analysis of many real samples with high background fluorescence.

show abstract

Microsecond-resolved smartphone time-gated luminescence spectroscopy

Cited by 4 publications

References 46 publications

High-Efficiency Pure Blue Circularly Polarized Phosphorescence from Chiral N-Heterocyclic-Carbene-Stabilized Copper(I) Clusters

High-Efficiency Pure Blue Circularly Polarized Phosphorescence from Chiral N-Heterocyclic-Carbene-Stabilized Copper(I) Clusters

Quantitative and Multiplexed Chopper-Based Time-Gated Imaging for Bioanalysis on a Smartphone

Sensitive and High-Throughput Time-Resolved Luminescence Detection of Tetracycline in Milk for Eliminating Background Fluorescence on a Miniaturized Apparatus

Contact Info

Product

Resources

About