Microsecond pulsed magnetic field improves efficacy of antifungal agents on pathogenic microorganisms

Novickij, Vitalij; Grainys, Audrius; Švedienė, Jurgita; Markovskaja, Svetlana; Paškevičius, Algimantas; Novickij, Jurij

doi:10.1002/bem.21848

Cited by 10 publications

(8 citation statements)

References 31 publications

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“…DC electromagnets are usually the most used for assistance in processes to eliminate water pollutants, and they are completely similar to AC electromagnets, with the difference that the field they generate is homogeneous and continuous due to the nature of the electric current, although they can also generate pulsations [99][100][101]. Studies have been carried out on the application of an electromagnet in adsorption processes, where it has been found that the field generated by them decreases the equilibrium time [101], increases the efficiency of dye removal by orienting the molecules and creating micromagnets from magnetic adsorbent materials [102].…”

Section: Generation and Configuration Of A Magnetic Field Applied To mentioning

confidence: 99%

Removal of Heavy Metals Using Adsorption Processes Subject to an External Magnetic Field

Virgen¹,

Vázquez²,

Montoya³

et al. 2018

Heavy Metals

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Adsorption is a broadly used process for the removal of heavy metals and the world trend is directed to the application of new technologies to intensify existing processes. The properties of the magnetic field (intensity and arrangement) and the intrinsic magnetic properties of the adsorbent and the adsorbate are decisive for satisfactory results. The intensity of the magnetic field is important, because this implies that the greater number of spins present will align with the magnetic field according to the magnetic nature present, allowing the mobility of the adsorbate and generating heterogeneity on the surface of the adsorbent. Similarly, the arrangement of the magnetic field will determine the direction of the magnetic field lines. The application of a magnetic field as an alternative for the intensification of the adsorption process based on the consideration that the magnetic field is safe, environmentally friendly and economic.

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Section: Generation and Configuration Of A Magnetic Field Applied To mentioning

confidence: 99%

Removal of Heavy Metals Using Adsorption Processes Subject to an External Magnetic Field

Virgen¹,

Vázquez²,

Montoya³

et al. 2018

Heavy Metals

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“…In 2012 Kardos and Rabussay have used 50 -75 µs magnetic field pulses up to 4 T for intracellular plasmid DNA delivery in vivo [13]. In our previous works we have shown that the pulsed magnetic field causes irreversible cell membrane permeabilization [12,17].…”

Section: Introductionmentioning

confidence: 98%

Magneto-Permeabilization of Viable Cell Membrane Using High Pulsed Magnetic Field

et al. 2015

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In this work we present novel experimental data of the magnetic permeabilization of the mammalian cells in high pulsed magnetic fields up to 16.4 T. Two designs of the multilayer solenoid type pulsed inductors, which were adapted for the biological experiments are presented. The induced electric field is analyzed using the finite element method analysis. The experimental methodology and the pulsed magnetic field setups are overviewed. The experimental results with mouse myeloma cell line Sp2/0 in pulsed magnetic field after pulse bursts and single pulse treatment are presented. The SYTOX ® Green dye was used for the estimation of the permeabilization process efficacy. It has been shown that the 16.4 T sub-millisecond low (< 1.1×10 5 T/s) dB/dt magnetic field pulses do not cause any observable dye uptake changes using the proposed methodology. The short repetitive microsecond range pulses (dB/dt > 0.8×10 6 T/s) result in an average up to 2.6±0.5% increase of the dye uptake.

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“…large number of 25 up to 800 the μs long magnetic field pulses applied at frequencies from 25 Hz up to 40 Hz and strength from 725 V/m up to 160 kV/m. 30 , 35 , 36 Furthermore, its use as electroporation tool was shown in an approach for drug as well as for plasmid DNA delivery. 36 , 37 Thus, such electromagnetic induction with alternating currents has the potential for simple contactless tissue electroporation, used for electrochemotherapy and gene electrotransfer.…”

Section: Introductionmentioning

confidence: 99%

“… 30 , 34 In a recent study, time varying magnetic field of 6.1 T was shown as an interesting tool in drug delivery for antifungal treatment, as well as for irreversible electroporation. 36 , 38 Furthermore, the bipolar pulses generated by magnetic field (4 T) were used for gene electrotransfer of plasmid DNA into the skin. 37 Thus, as simple, noninvasive and contactless application of PEMF, which could enable electroporation of cells in the tissue, this approach showed the potential use for the clinical applications.…”

Section: Introductionmentioning

confidence: 99%

Electrochemotherapy by pulsed electromagnetic field treatment (PEMF) in mouse melanoma B16F10 in vivo

Kranjc

Ščančar

et al. 2016

Radiology and Oncology

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IntroductionPulsed electromagnetic field (PEMF) induces pulsed electric field, which presumably increases membrane permeabilization of the exposed cells, similar to the conventional electroporation. Thus, contactless PEMF could represent a promising approach for drug delivery.Materials and methodsNoninvasive electroporation was performed by magnetic field pulse generator connected to an applicator consisting of round coil. Subcutaneous mouse B16F10 melanoma tumors were treated with intravenously injection of cisplatin (CDDP) (4 mg/kg), PEMF (480 bipolar pulses, at frequency of 80 Hz, pulse duration of 340 μs) or with the combination of both therapies (electrochemotherapy − PEMF + CDDP). Antitumor effectiveness of treatments was evaluated by tumor growth delay assay. In addition, the platinum (Pt) uptake in tumors and serum, as well as Pt bound to the DNA in the cells and Pt in the extracellular fraction were measured by inductively coupled plasma mass spectrometry.ResultsThe antitumor effectiveness of electrochemotherapy with CDDP mediated by PEMF was comparable to the conventional electrochemotherapy with CDDP, with the induction of 2.3 days and 3.0 days tumor growth delay, respectively. The exposure of tumors to PEMF only, had no effect on tumor growth, as well as the injection of CDDP only. The antitumor effect in combined treatment was related to increased drug uptake into the electroporated tumor cells, demonstrated by increased amount of Pt bound to the DNA. Approximately 2-fold increase in cellular uptake of Pt was measured.ConclusionsThe obtained results in mouse melanoma model in vivo demonstrate the possible use of PEMF induced electroporation for biomedical applications, such as electrochemotherapy. The main advantages of electroporation mediated by PEMF are contactless and painless application, as well as effective electroporation compared to conventional electroporation.

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Microsecond pulsed magnetic field improves efficacy of antifungal agents on pathogenic microorganisms

Cited by 10 publications

References 31 publications

Removal of Heavy Metals Using Adsorption Processes Subject to an External Magnetic Field

Removal of Heavy Metals Using Adsorption Processes Subject to an External Magnetic Field

Magneto-Permeabilization of Viable Cell Membrane Using High Pulsed Magnetic Field

Electrochemotherapy by pulsed electromagnetic field treatment (PEMF) in mouse melanoma B16F10 in vivo

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