Microscopic view of osseointegration and functional mechanisms of implant surfaces

Abstract

Argon ion beam polishing technique was applied to prepare the cross sections of implants feasible for high resolution scanning electron microscope investigation. The interfacial microstructure between newly formed bone and implants with three modified surfaces retrieved after in vivo test using three different animal models was characterized. By this approach it has become possible to directly observe early bone formation, the increase of bone density, and the evolution of bone structure. The two bone growth mechanisms, distant osteogenesis and contact osteogenesis, can also be distinguished. These direct observations give, at microscopic level, a better view of osseointegration and expound the functional mechanisms of various implant surfaces for osseointegration.

Multimodal Evaluation of the Spatiotemporal Variations of Periprosthetic Bone Properties

“…Our results showing a bone formation starting in the upper corners of the bone chamber seem in better agreement with the contact osteogenesis scenario, since blood is likely to accumulate in L1 after surgery, providing the osteogenic cells needed for bone formation [62,65]. A predominant contact osteogenesis is expected when rougher surfaces are employed [66], as such surfaces offer more space for cells to attach to the implant surface, thus promoting bone cell migration [67]. Furthermore, bone does not only grow in contact with titanium, but also along PTFE on the lateral sides of the bone chamber.…”

Osseointegration behavior of carbon fiber reinforced polyetheretherketone composites modified with amino groups: An in vivo study

“…The fluorescence of implant‐tissue sections was observed and photographed by a confocal laser scanning microscope (CLSM) (OLYMPUS FV3000, Japan). Generally speaking, the excitation wavelengths of tetracycline hydrochloride and alizarin red are about 365–436 and 530–580 nm, and the emission wavelengths are approximately 570 and 600–645 nm, 22 with yellow and red emission spectra, respectively. After capturing images, the histomorphometric evaluation was performed using Image‐Pro Plus Vision 6.0 image analysis software by measuring the distance between different fluorescent labelings.…”

The experimental study of TNF-α & CRP expression in the spinal tuberculosis after instrumentation

“…Macrophage activation can be induced or regulated by the characteristics of the implant surface, which will eventually change the healing activity and affect the long-term stability of the implant. A chronic immune response prevents the formation of healthy tissue around the implant [ [14] , [15] , [16] ]. This study aimed to investigate the expression of TNFα and CRP in Tuberculous Spondylitis (TB) and its relationship with instrumentation.…”