Microscopic Study of Testicular Tissue Structure and Spermatogenesis Following Long Term Dose Dependent Administration of Monosodium Glutamate in Adult Diabetic Rats

Kianifard, Davoud

doi:10.1515/rjdnmd-2016-0018

Cited by 7 publications

(7 citation statements)

References 33 publications

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“…Thus, the decrease in testicular weight is related to the decrease in the number of germ and somatic cells in testicles. Comparable results have been reported by Shojaeii et al and Kianifard (Shojaeii et al, 2014;Kianifard, 2016).…”

Section: Discussionsupporting

confidence: 78%

Effects of Diabetes Mellitus on the Reproductive System of Adult Male Mice After One Cycle of Spermatogenesis

Bégbin

Zougrou

Abizi

et al. 2021

ESJ

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Background: Diabetes mellitus is a disease characterized by chronic hyperglycemia. Poor management of hyperglycemia leads to complications that can reduce quality of life. Diabetes mellitus has been associated with damage to the reproductive system. The present study examines effects of diabetes mellitus on the reproductive system in alloxan-induced diabetic mice after one cycle of spermatogenesis. Materials and Methods: Twelve (12) mice divided into "control" and "diabetic" groups of six (6) animals each were used. Diabetes was induced in adult male mice by intraperitoneal injection with a single dose of 220 mg/kg body weight of alloxan. After 40 days, sperm density, morphology, and motility were assessed by standard methods. Serum levels of testosterone, FSH, and LH were measured. In addition, testes and epididymis were removed for histological study. Results: Highly significant (P < 0.001) reductions in serum testosterone, FSH, and LH levels, as well as normal and motile sperm counts of 21% and 34% respectively were observed in the diabetic group. The control group had three times more sperm than the diabetic group. The histological increased inter-tubular spaces, loss of interstitial tissue, degeneration of seminiferous tubules, and almost complete reduction of sperm count in the epididymal duct. Conclusion: Chronic hyperglycemia is therefore deleterious to the male reproductive system of mice. It leads to hypogonadism, which causes dysfunction of the male reproductive system, and can lead to infertility in men with diabetes mellitus.

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Section: Discussionsupporting

confidence: 78%

Effects of Diabetes Mellitus on the Reproductive System of Adult Male Mice After One Cycle of Spermatogenesis

Bégbin

Zougrou

Abizi

et al. 2021

ESJ

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“…The histopathological lesions in testicular tissues exposed to MSG is similar to those observed by other investigators 49 . The depopulation of germ cells due to inhibition of cell division is responsible for atrophy of ST 87 . The degenerative changes in germ and somatic cells of ST may be arisen from the interaction of MSG with proteins and enzymes interfering with the antioxidant defense mechanism leading to accumulation of free radicals which in turn induces inflammatory reaction and mitochondrial damage 88 .…”

Section: Discussionmentioning

confidence: 99%

Antioxidant and cytoprotective effects of Nigella sativa L. seeds on the testis of monosodium glutamate challenged rats

Abd-Elkareem

El-Rahman

Khalil

et al. 2021

Sci Rep

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Monosodium glutamate (MSG) is one of the most widely spread food additives that might cause male infertility. However, Nigellasativa L. seeds (NSS) could provide a solution. This study was designed to investigate the potential effects of NSS on rats ingesting MSG. To achieve this aim, adult male albino rats were randomly equally assigned into three groups for 21 days: control group received no treatment, MSG group received MSG as 30 g/kg feed, and MSG + NSS group received MSG as 30 g/kg and NSS as 30 g/kg feed. Testis histomorphometry showed marked deterioration by MSG as atrophic seminiferous tubules with degeneration of their lining cells, damaged Leydig cells and decreased germ cells number. Periodic Acid Schiff stain indicated irregular interrupted basement membranes. Glutathione reductase, superoxide dismutase 2 (SOD2), and caspase-3 immuno-expressions increased in testicular cells. Testosterone levels were significantly decreased in MSG challenged rats along with significant increase in luteinizing hormone levels, whereas NSS normalized this hormonal profile. MSG exposure also caused significantly increased lipid peroxides (LPO), glutathione-S-transferase, and total antioxidant capacity (TAC) whereas nitric oxide and SOD2 were significantly decreased. NSS succeeded in rebalance LPO and TAC and ameliorated the histoarchitectural disturbances. NSS mitigated MSG-induced testicular impairment by its antioxidant and cytoprotective activities.

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“…Such findings were in consistent with previous studies on the effect of MSG in rat testis. [ 10 36 ] Kianifard[ 37 ] observed reduction in the diameter of STs and decreased number of primary spermatocytes and spermatids following MSG treatment in rats and mentioned that depopulation of germ cells led to tubular atrophy and indicated a reduction of cell divisions. The widening in the intertubular space that was observed in this study might be due to the atrophy and reduced tubular diameter.…”

Section: Discussionmentioning

confidence: 99%

“…MSG is neurotoxic, when found in high concentration it causes excessive glutamate receptor activation and can induce neuronal death. [ 43 ] Hypothalamic neuronal cell death[ 44 ] and reduction in the level of luteinizing hormone (LH), FSH, and testosterone and decreased sperm count[ 36 37 38 ] were previously reported after administration of MSG in mice and rats. Hence, the damage of hypothalamic neurons by MSG may cause disruption of the hypothalamic–pituitary–gonadal axis that control the normal spermatogenesis and testosterone secretion by the Leydig cells[ 45 ] and can lead to structural and functional alterations of testicular tissue.…”

Section: Discussionmentioning

confidence: 99%

The ameliorating effect of sodium selenite on the histological changes and expression of caspase-3 in the testis of monosodium glutamate-treated rats: Light and electron microscopic study

Sarhan

2018

J Microsc Ultrastruct

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Monosodium glutamate (MSG) is a commonly used flavor enhancer that may contribute to male infertility. Sodium selenite is inorganic chemical form of selenium (Se). Se is best known as an antioxidant. This study was designed to investigate the possible ameliorating role of sodium selenite against MSG-induced testicular toxicity and histological changes. Forty male albino rats were allocated into four groups. Control group received distilled water, SE group received sodium selenite (0.25 mg/kg/day) dissolved in distilled water orally, MSG group received MSG (6 mg/g/day) dissolved in distilled water orally, and MSG + SE group received both MSG and sodium selenite for 45 days. Testicular samples were prepared for biochemical, light, and electron microscopic studies. Immunohistochemical staining for caspase-3 was done. MSG group demonstrated a significant increase in malondialdehyde level, marked damage of seminiferous tubules with a significant reduction in diameter and height of the lining epithelium. Spermatogenic cells showed disorganization, dark nuclei, reduction in number, and maturation arrest. Vacuolations of interstitial tissue and Leydig cells were also observed. Percent area of fibrosis and caspase-3 immunoexpression was significantly increased. Ultrastructurally, irregular tubular basement membrane and damaged germ cells were found. The spermatogenic, Sertoli, and Leydig cells showed irregular shrunken nuclei, cytoplasmic vacuolations, and swollen mitochondria. MSG + SE group showed much better histological and ultrastructural picture and improvement of the measured biochemical and morphometric parameters. Percent area of caspase-3 immunoexpression was significantly decreased. In conclusion, sodium selenite ameliorated the testicular damaging effect of MSG through reduction of oxidative stress and apoptosis.

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Microscopic Study of Testicular Tissue Structure and Spermatogenesis Following Long Term Dose Dependent Administration of Monosodium Glutamate in Adult Diabetic Rats

Abstract: Background and aims: Diabetic hyperglycemia leads to structural and functional alterations in body organs including testis. Monosodium glutamate (MSG) is a food

Cited by 7 publications

References 33 publications

Effects of Diabetes Mellitus on the Reproductive System of Adult Male Mice After One Cycle of Spermatogenesis

Effects of Diabetes Mellitus on the Reproductive System of Adult Male Mice After One Cycle of Spermatogenesis

Antioxidant and cytoprotective effects of Nigella sativa L. seeds on the testis of monosodium glutamate challenged rats

The ameliorating effect of sodium selenite on the histological changes and expression of caspase-3 in the testis of monosodium glutamate-treated rats: Light and electron microscopic study

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