Microscopic profile control mechanism and potential application of the biopolymer-producing strain FY-07 for microbial enhanced oil recovery

Bi, Yongqiang; Yu, Li; Huang, Lihua; Ma, Ting; Xiu, Jianlong; Yi, Lina

doi:10.1080/10916466.2016.1233249

Cited by 8 publications

(9 citation statements)

References 10 publications

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“…To date, a large amount of microbial populations have been isolated or detected in oil reservoirs worldwide (Lenchi et al, 2013; Al-Sayegh et al, 2015; Gao et al, 2016; Okoro et al, 2016; Su et al, 2018). These microorganisms are versatile: some species can degrade crude oil, some can produce biosurfactants (Liu et al, 2015; Cui et al, 2017; Qi et al, 2018), biopolymers (Bi et al, 2016; Xia et al, 2018; Zhao et al, 2018), or biogases (Dong et al, 2015; Zhu et al, 2015), and have been used to mobilize and recover residual oil from oil-bearing rocks (Youssef et al, 2009; Wang et al, 2014; Yi et al, 2018). In addition, nitrate can be used to reduce formation of hydrogen sulfide produced by sulfate-reducing bacteria (SRB) based on the competitive inhibition of nitrate-reducing bacteria on SRB (Gieg et al, 2011; Gassara et al, 2015).…”

Section: Introductionmentioning

confidence: 99%

Composition of Bacterial and Archaeal Communities in an Alkali-Surfactant-Polyacrylamide-Flooded Oil Reservoir and the Responses of Microcosms to Nutrients

Gao

Tan

et al. 2019

Front. Microbiol.

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The microbial communities in alkali-surfactant-polyacrylamide-flooded (ASP-flooded) oil reservoirs have rarely been investigated compared to those in water-flooded oil reservoirs. Here, the bacterial and archaeal communities in an ASP-flooded reservoir and the adjacent water-flooded block, and responses of the microbial communities in microcosms to nutrients were investigated by 16S rRNA gene sequencing and cultivation. Compared with the water-flooded block, both the bacterial and archaeal communities inhabiting the ASP-flooded block had lower Sobs indices (91:232 and 34:55, respectively), lower Shannon indices (1.296:2.256 and 0.845:1.627, respectively) and higher Simpson indices (0.391:0.248 and 0.678:0.315, respectively). Halomonas (58.4–82.1%) and Anoxynatronum (14.5–18.2%) predominated in the ASP-flooded production wells, and were less than 0.05% in the bacterial communities of the adjacent water-flooded production wells, which were dominated by Pseudomonas and Thauera. Methanobacterium accounted for 65.0–94.5% of the archaeal communities inhabiting the ASP-flooded production wells, and Methanosaeta (36.7–94.5%) dominated the adjacent water-flooded production wells. After nutrients stimulation, the quantity of cultivable microorganisms increased from 103/mL to 107/mL. Community analysis indicated that the relative abundances of some species that belonged to Halomonas and Pseudomonas obviously increased, yet there were no oil emulsification or dispersion and changes of surface tension of the water-oil mixture. In addition, 6 alkali-tolerating strains showing 98% similarity of 16S rRNA genes with those of Halomonas alkalicola and Halomonas desiderata and 2 strains with 99% similarity with Pseudomonas stutzeri gene were isolated from the nutrients stimulated brines. In summary, this study indicated that Halomonas, Anoxynatronum, and Methanobacterium were dominant populations in the ASP-flooded reservoir, the extreme environment decreased microbial diversity, and restricted microbial growth and metabolisms.

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Section: Introductionmentioning

confidence: 99%

Composition of Bacterial and Archaeal Communities in an Alkali-Surfactant-Polyacrylamide-Flooded Oil Reservoir and the Responses of Microcosms to Nutrients

Gao

Tan

et al. 2019

Front. Microbiol.

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“…However, due to the heterogeneity of reservoir structure, only microbial flooding is inefficient, because the injected microbial fluids move along the high permeability layer with relatively low oil saturation, resulting in low oildisplacement efficiency [27]. In some studies, no obvious oil recovery was also observed if a short retention period was carried out after microbial flooding [28,29].…”

Section: Introductionmentioning

confidence: 99%

“…Ansai Oilfield, the first oilfield with 100 million tons of integrated ultra-low permeability on land in China, has typical "three-low" characteristics, and is known as a grindstone oilfield with "oil in wells but no flow in wells" [29]. Massive obvious micro-fractures are developed in this oilfield.…”

Section: Introductionmentioning

confidence: 99%

Improving Oil Recovery of the Heterogeneous Low Permeability Reservoirs by Combination of Polymer Hydrolysis Polyacrylamide and Two Highly Biosurfactant-Producing Bacteria

Xue

Zhao²,

Zhou³

et al. 2021

Sustainability

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Polymer hydrolysis polyacrylamide and microbes have been used to enhance oil recovery in many oil reservoirs. However, the application of this two-method combination was less investigated, especially in low permeability reservoirs. In this work, two bacteria, a rhamnolipid-producing Pseudomonas aeruginosa 8D and a lipopeptide-producing Bacillus subtilis S4, were used together with hydrolysis poly-acrylamide in a low permeability heterogeneous core physical model. The results showed that when the two bacterial fermentation liquids were used at a ratio by volumeof 1:3 (v:v), the mixture showed the optimal physicochemical properties for oil-displacement. In addition, the mixture was stable under the conditions of various temperature (20–70 °C) and salinity (0–22%). When the polymer and bacteria were mixed together, it had no significant effects in the viscosity of polymer hydrolysis polyacrylamide and the viability of bacteria. The core oil-displacement test displayed that polymer hydrolysis polyacrylamide addition followed by the bacterial mixture injection could significantly enhance oil recovery. The recovery rate was increased by 15.01% and 10.03%, respectively, compared with the sole polymer hydrolysis polyacrylamide flooding and microbial flooding. Taken together, these results suggest that the strategy of polymer hydrolysis poly-acrylamide addition followed by microbial flooding is beneficial for improving oil recovery in heterogeneous low permeability reservoirs.

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“…MEOR mainly improves the oil recovery by increasing the displacement efficiency and the sweep volume of reservoir [12]. Some scholars have screened the different biosurfactant-producing bacteria for improving the oil displacement efficiency [4,13,14], while others used the biopolymer-producing bacteria to improve the sweep efficiency [8,15,16]. Qi et al studied the compatibility between the W5 strains and the weak gel and highlighted the application potential of weak gel-assisted microbial flooding [17].…”

Section: Introductionmentioning

confidence: 99%

Application Potential Analysis of Enhanced Oil Recovery by Biopolymer-Producing Bacteria and Biosurfactant-Producing Bacteria Compound Flooding

Xiu

2019

Applied Sciences

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To study the feasibility of polymer-producing bacteria Enterobacter cloacae (E. cloacae) FY-07 and surfactant-producing bacteria Pseudomonas aeruginosa WJ-1 combined profile control and flooding, the compatibility of FY-07 and WJ-1 was evaluated using laboratory experiments. The results showed that the growth and metabolism of WJ-1 was not significantly affected by the FY-07 in the degradation medium, and the surface tension of fermentation broth was reduced from 70 mN/m to 30 mN/m. FY-07 enhanced the degradation of WJ-1, increasing the ratio of C14- to C15+ from 0.37 to 0.67. The core-flooding experiments indicated the oil recovery of 17.4% when both FY-07 and WJ-1 were injected into the system, as against to 10.4% and 7.9% for FY-07 and WJ-1, respectively, when injected alone. The results demonstrate a good compatibility between the FY-07 and WJ-1 strains and highlight the application potential of stain FY-07 and strain WJ-1 compound flooding for enhancing the oil recovery in heterogeneous reservoirs.

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Microscopic profile control mechanism and potential application of the biopolymer-producing strain FY-07 for microbial enhanced oil recovery

Cited by 8 publications

References 10 publications

Composition of Bacterial and Archaeal Communities in an Alkali-Surfactant-Polyacrylamide-Flooded Oil Reservoir and the Responses of Microcosms to Nutrients

Composition of Bacterial and Archaeal Communities in an Alkali-Surfactant-Polyacrylamide-Flooded Oil Reservoir and the Responses of Microcosms to Nutrients

Improving Oil Recovery of the Heterogeneous Low Permeability Reservoirs by Combination of Polymer Hydrolysis Polyacrylamide and Two Highly Biosurfactant-Producing Bacteria

Application Potential Analysis of Enhanced Oil Recovery by Biopolymer-Producing Bacteria and Biosurfactant-Producing Bacteria Compound Flooding

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