Microscopic models of liquid chromatography: From ensemble-averaged information to resolution of fundamental viewpoint at single-molecule level

Pasti, Luisa; Marchetti, Nicola; Guzzinati, Roberta; Catani, Martina; Bosi, Valentina; Dondi, Francesco; Sepsey, Annamária; Felinger, Attila; Cavazzini, Alberto

doi:10.1016/j.trac.2015.08.007

Cited by 10 publications

(5 citation statements)

References 38 publications

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“…Accordingly, the time spent by a molecule inside the column is the sum of the times spent by the molecule in the stationary phase and those elapsed in the mobile phase between two successive adsorptions. 44,[56][57][58][59][60] From the analysis of the peak shape, the stochastic model allows for the estimation of both the average adsorption time, τ s , and the flying time, as well as of the number of adsorption-desorption steps. Thus, from the average adsorption time, the estimation of k ads is possible, as follows: 42…”

Section: Mass Transfer In Chiral Chromatographymentioning

confidence: 99%

Recent advancements and future directions of superficially porous chiral stationary phases for ultrafast high-performance enantioseparations

Catani

Ismail

Antonelli

et al. 2017

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This review focuses on the use of superficially porous particles (SPPs) as chiral stationary phases for ultra-high performance liquid enantioseparations. In contrast to what happened in achiral separations where core-shell particles invaded the market, the introduction of SPPs in chiral liquid chromatography (LC) has been relatively recent. This is due in part to the technical difficulties in the preparation of these phases, and in part to scarce understanding of mass transfer phenomena in chiral chromatography. As a matter of fact, nowadays, the development of superficially porous CSPs is still in its infancy. This paper covers the most recent advancements in the field of core-shell technology applied to chiral separations. We review the kinds of chiral selectors that have been used for the preparation of these phases, by discussing the advantages of chiral SPPs over their fully-porous counterparts for high efficient high throughput enantioseparations. Notwithstanding the apparently obvious advantages in terms of the mass transfer of chiral SPPs, some critical aspects that could impact their development are presented.

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Section: Mass Transfer In Chiral Chromatographymentioning

confidence: 99%

Recent advancements and future directions of superficially porous chiral stationary phases for ultrafast high-performance enantioseparations

Catani

Ismail

Antonelli

et al. 2017

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“…From a fundamental viewpoint, an intrinsic complication in chiral chromatography comes from the fact that the contribution to mass transfer coming from adsorption-desorption kinetics is very difficult to quantify. On the other hand, it is widely acknowledged that this is very often the main source of band broadening even at low flow rates and even for small molecules [15][16][17][18][19][20]. The main difference between chiral and achiral separations is indeed in the impact of adsorption-desorption kinetics on efficiency.…”

Section: Introductionmentioning

confidence: 99%

Mass transfer kinetics on modern Whelk-O1 chiral stationary phases made on fully- and superficially-porous particles

Felletti

Catani

Mazzoccanti

et al. 2021

Journal of Chromatography A

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“…They concern essentially two interconnected aspects. The first one is about the importance of the adsorption-desorption kinetics on the performance of modern, ultra-high effient chiral LC columns [47,48]. In particular, questions such as:…”

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confidence: 99%

New frontiers and cutting edge applications in ultra high performance liquid chromatography through latest generation superficially porous particles with particular emphasis to the field of chiral separations

et al. 2018

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About ten years after their introduction to the market (happened in 2006), the so-called second generation superficially porous particles (SPPs) have undoubtedly become the benchmark as well as, very often, the preferred choice for many applications in liquid chromatography (LC), when high efficiency and fast separations are required. This trend has interested practically all kinds of separations, with the only exception of chiral chromatography (at least so far). The technology of production of base SPPs is advanced, relatively simple and widely available. The deep investigation of mass transfer mechanisms under reversed-phase (RP) and normal-phase (NP) conditions for achiral separations has shown the advantages in the use of these particles over their fully porous counterparts. In addition, it has been demonstrated that SPPs are extremely suitable for the preparation of efficient packed beds through slurry packing techniques. However, the research in this field is in continual evolution. In this article, some of the most advanced concepts and modern applications based on the use of SPPs, embracing in particular ultrafast chiral chromatography and the design of SPPs with engineered pore structures or very reduced particle diameter, are revised. We describe modern trends in these fields and focus on those aspect where further innovation and research will be required. Graphical Abstract Word cloud of cutting edge applications of superficially porous particles in liquid chromatography.

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Microscopic models of liquid chromatography: From ensemble-averaged information to resolution of fundamental viewpoint at single-molecule level

Cited by 10 publications

References 38 publications

Recent advancements and future directions of superficially porous chiral stationary phases for ultrafast high-performance enantioseparations

Recent advancements and future directions of superficially porous chiral stationary phases for ultrafast high-performance enantioseparations

Mass transfer kinetics on modern Whelk-O1 chiral stationary phases made on fully- and superficially-porous particles

New frontiers and cutting edge applications in ultra high performance liquid chromatography through latest generation superficially porous particles with particular emphasis to the field of chiral separations

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