Microscopic investigation to determine the effect of Beauveria bassiana (Bals.) Vuill. and Cymbopogon citratus (DC.) Stapf. treatment on different life stages of Musca domestica (L.)

Mishra, Sapna; Kumar, Pawan; Malik, Anushree

doi:10.1007/s12639-016-0848-4

Cited by 8 publications

(4 citation statements)

References 23 publications

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“…domestica and other insects [35][36][37][38][39] . In addition, C. citratus EO acted toxically on the integument of M. domestica larvae, causing cell shrinkage, spinous cells proliferation, incomplete emergence of pupae, and abnormality of adults 40 . I. verum EO, geranial, and trans-anethole destroyed cell membranes, reduced cell metabolism, and had a neurotoxic inhibition and reduction of acetylcholinesterase (AChE) activity, including accumulation of acetylcholine in synapses of the nervous system of insects 35 and M.…”

Section: Discussionmentioning

confidence: 99%

Synergistic toxicity against housefly Musca domestica of plant essential oils and their major constituents and safety against a non-target pollinator

Moungthipmalai

Puwanard

Sittichok

et al. 2023

Preprint

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Plant essential oils (EOs) can fulfill the role of safe and effective fly repellent and adulticide. Individual and binary mixture formulations of Cymbopogon citratus (Stapf.) and Illicium verum (J. Presl.) EOs and their major constituents were assayed for their repellent and adulticidal activities against Musca domestica L. and non-target pollinator, Apis florea F. Their efficacy was compared to that of 1.0% (w/v) α-cypermethrin. A GC-MS analysis found that geranial and trans-anethole were the major constituents of C. citratus EO (45.23%) and I. verum (93.23%), respectively. All binary mixture formulations exhibited high and synergistic repellent and adulticidal effects: an increased repellent value between 18.7 to 57.6%, and a mortality rate value between 41.9 to 92.7%. The highest synergistic repellent effect was from 2.5% C. citratus EO + 2.5% I. verum EO, while the highest synergistic adulticidal effect was from 1.0% C. citratus EO + 1.0% trans-anethole. Both were more effective than 1.0% (w/v) α-cypermethrin. On the other hand, they were not toxic to the non-target pollinator. To conclude, C. citratus EO + I. verum EO and C. citratus EO + trans-anethole have a high potential as repellent and adulticidal agents against M. domestica which are safe for a pollinator, Apis florea.

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Section: Discussionmentioning

confidence: 99%

Synergistic toxicity against housefly Musca domestica of plant essential oils and their major constituents and safety against a non-target pollinator

Moungthipmalai

Puwanard

Sittichok

et al. 2023

Preprint

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“…Whole-cell suspensions and crude cells were applied at six concentrations, 3×10 4 , 3×10 5 , 3×10 6 , 3×10 7 , 3×10 8 and 3×10 9 cells mLG 1 . While cell-free supernatant was applied at concentrations of 5,20,40,60,80 and 100% by diluting cell-free supernatants with distilled water on a v/v basis using 10 mL falcon tubes. Two milliliters of each concentration from each treatment was mixed well with 20 g of housefly larvae nutrient media in 9×3 cm plastic dishes containing 15 sec instar housefly larvae starved for 12 hrs before application, treatment was replicated five times.…”

Section: Preparation Of Cell-free Bacterial Supernatant (Metabolites)mentioning

confidence: 99%

“…and Heterorhabditis spp.) 17,18 , fungi (Beauveria bassiana) 19,20 , bacteria (e.g., Bacillus thuringiensis) [21][22][23] , essential oils and plant extracts [24][25][26] .…”

Section: Introductionmentioning

confidence: 99%

Photorhabdus and Xenorhabdus as Symbiotic Bacteria for Bio-Control Housefly (Musca domestica L.)

El-Rah¹,

Abdelazeem²,

Elbrense³

et al. 2022

Pakistan J. of Biological Sciences

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Background and Objective: The housefly poses a threat to the public health of humans and domestic animals since it can carry and transmit pathogens. Despite there are many attempts to control this insect, most of them depend on conventional pesticides. Thus, the current study aimed to evaluate the efficacy of whole-cell suspension, cell-free supernatant and crude cells of the symbiotic bacteria Photorhabdus sp. and Xenorhabdus sp., as bio-control agents for housefly stages. Materials and Methods: The Photorhabdus sp. and Xenorhabdus sp., were isolated from the entomopathogenic nematodes, Heterorhabditis indica and Steinernema feltiae, respectively.The phenotypic, as well as the enzymatic characterizations of both bacteria, were determined. In addition, histopathological changes of the alimentary canal of M. domestica adults treated with whole-cell suspensions (at 3×10 8 cells mLG 1 ) of both bacteria were carefully examined using transmission electron microscopy. Results: The results showed that both symbiotic bacteria significantly suppressed larvae, pupae and adults of M. domestica, particularly when they were applied as whole-cell suspensions. For example, the highest concentration of whole-cell suspension, cell-free supernatant and crude cells of Photorhabdus sp., induced larval mortalities by 94.7, 64.0 and 45.3%, while those of Xenorhabdus sp., induced larval mortalities by 58.7, 46.7 and 30.7% at 96 hrs, respectively. The results also showed that whole-cell suspensions of both symbiotic bacteria caused severe histopathological changes in the ultrastructure of the treated adultsʼ alimentary canal. Conclusion: Both symbiotic bacteria can be effectively used, particularly the whole-cell suspension, as bio-control agents against the housefly either in the larval or adult stage.

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“…Subsequent to attachment, spores germinate on the insect cuticle, and then the germinated conidia (germ tube) penetrate into the cuticle. After penetration, fungi inhibit the host immune system and grow in the hemocoel, taking up nutrients, producing toxins, destroying host cells and eventually killing the insect (22)(23)(24). After infection, the gut bacteria are also altered by B. bassiana, such as in mosquitoes (25), cockroaches (5), Delia antiqua (26) and locusts (27).…”

Section: Introductionmentioning

confidence: 99%

Dysbacteriosis of the Intestinal Flora Is an Important Reason for the Death of Adult House Flies Caused by Beauveria bassiana

et al. 2021

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Beauveria bassiana is one of the most widespread insect pathogens and can be used in the biological control of agricultural, forestry and medical pests. The mechanisms by which B. bassiana leads to mortality in different host insects are also different. For house flies, B. bassiana has strong virulence, but its microecological mechanism is not clear. In this paper, the virulence of three strains of B. bassiana (TB, CB and BB) isolated from different hosts to house flies was studied. The results showed that the three strains of B. bassiana had strong pathogenicity to house fly adults. Specifically, TB was the strongest, CB was the second strongest, and BB was the weakest, with maximum lethal effects on house fly populations 5, 6, and 7 days after infection, respectively. Further study showed that the intestinal flora of house flies was disordered 3, 4, and 5 days after B. bassiana TB, CB and BB strain infection, respectively. Intestinal flora dysbacteriosis may be an important reason for the death of house flies caused by B. bassiana. After infection, the negative interaction ratio of bacteria in the house fly intestine decreased, and the stronger the virulence was, the lower the negative interaction ratio was. The time from B. bassiana infection to intestinal flora dysbacteriosis was not fixed. We named this period the “spring stage”. The stronger the virulence of the B. bassiana strain was, the shorter the “spring stage” was. Therefore, the “spring stage” can be used as a virulence marker for evaluating the pathogenicity of different B. bassiana strains.

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Microscopic investigation to determine the effect of Beauveria bassiana (Bals.) Vuill. and Cymbopogon citratus (DC.) Stapf. treatment on different life stages of Musca domestica (L.)

Cited by 8 publications

References 23 publications

Synergistic toxicity against housefly Musca domestica of plant essential oils and their major constituents and safety against a non-target pollinator

Synergistic toxicity against housefly Musca domestica of plant essential oils and their major constituents and safety against a non-target pollinator

Photorhabdus and Xenorhabdus as Symbiotic Bacteria for Bio-Control Housefly (Musca domestica L.)

Dysbacteriosis of the Intestinal Flora Is an Important Reason for the Death of Adult House Flies Caused by Beauveria bassiana

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