Microscopic characterization of biofilm in mixed keratitis in a novel murine model

Ponce-Angulo, Diana Gabriela; Bautista-Hernández, Luis Antonio; Calvillo‐Medina, Rosa Paulina; Castro-Tecorral, Franco Ivan; Aparicio-Ozores, Gerardo; López-Villegas, Edgar Óliver; Ribas‐Aparicio, Rosa María; Lucio, Víctor Manuel Bautista-de

doi:10.1016/j.micpath.2019.103953

Cited by 9 publications

(14 citation statements)

References 39 publications

Supporting

Mentioning

Contrasting

Order By: Relevance

“…Keratitis caused by this fungal pathogen is unusual, even in tropical countries like Mexico (5% of prevalence in a 6 year period in India) 37 . In developing countries, keratitis remains a health problem and is the leading cause of preventable blindness 32 . To our knowledge, this is the first report regarding Purpureocillium sp keratitis in Mexico.…”

Section: Discussionmentioning

confidence: 83%

“…The technique, described by Ponce‐Angulo et. al., was followed to promote the development of keratitis 32 For fungal infection, microorganisms were inoculated as described above at a concentration of 3 μl/ 1 × 10 5 conidia for Purpureocillium sp corneal isolate. According to the criteria of the IOFCV and ARVO ethics committees, only the right eye of each mouse was used in each group of this study.…”

Section: Methodsmentioning

confidence: 99%

See 1 more Smart Citation

Purpureocillium roseum sp. nov. A new ocular pathogen for humans and mice resistant to antifungals

Calvillo‐Medina

Ponce-Angulo

Raymundo

et al. 2020

Mycoses

View full text Add to dashboard Cite

BackgroundInfectious keratitis is the main cause of preventable blindness worldwide, with about 1.5–2.0 million new cases occurring per year. This inflammatory response may be due to infections caused by bacteria, fungi, viruses or parasites. Fungal keratitis is a poorly studied health problem.ObjectivesThis study aimed to identify a new fungal species by molecular methods and to explore the possible efficacy of the three most common antifungals used in human keratitis in Mexico by performing in vitro analysis. The capacity of this pathogen to cause corneal infection in a murine model was also evaluated.MethodsThe fungal strain was isolated from a patient with a corneal ulcer. To identify the fungus, taxonomic and phylogenetic analyses (nrDNA ITS and LSU data set) were performed. An antifungal susceptibility assay for amphotericin B, itraconazole and voriconazole was carried out. The fungal isolate was used to develop a keratitis model in BALB/c mice; entire eyes and ocular tissues were preserved and processed for histopathologic examination.Results and conclusionThis fungal genus has hitherto not been reported with human keratitis in Mexico. We described a new species Purpurecillium roseum isolated from corneal infection. P roseum showed resistance to amphotericin B and itraconazole and was sensitive to voriconazole. In vivo study demonstrated that P roseum had capacity to developed corneal infection and to penetrate deeper corneal tissue. The global change in fungal infections has emphasised the need to develop better diagnostic mycology laboratories and to recognise the group of potential fungal pathogens.

show abstract

Section: Discussionmentioning

confidence: 83%

Section: Methodsmentioning

confidence: 99%

Purpureocillium roseum sp. nov. A new ocular pathogen for humans and mice resistant to antifungals

Calvillo‐Medina

Ponce-Angulo

Raymundo

et al. 2020

Mycoses

View full text Add to dashboard Cite

show abstract

“…To the best of our knowledge, an in vitro model that combines live HCE cells and the formation of bacterial biofilm is yet to be reported. In contrast, multiple keratitis studies have investigated biofilm formation on abiotic surfaces in the absence of cells [131,132]. As in vitro modelling techniques continue to improve, co-culture models may be reported but there are various limitations associated with the use of in vitro systems for studying biofilm infections [133].…”

Section: Existing In Vitro Infection Modelsmentioning

confidence: 99%

“…In vivo modelling involves the use of live animals. Rat [171] and rabbit [172][173][174] models have been reported, but mouse models currently dominate the literature [131,137,138,[175][176][177][178]. Despite its smaller size, the murine cornea contains more corneal epithelial cell layers than the human cornea and the ratio of epithelial to stromal cells is larger [179].…”

Section: In Vivo Modelsmentioning

confidence: 99%

“…Increasing interest in ocular biofilms over the past decade has resulted in the development of an established in vivo cornea model [177], followed by improved methods of imaging bacteria and biofilm formation [22,131,137,139,176,181]. This has allowed researchers to begin to characterise the process of biofilm formation at the ocular surface (Table 2).…”

Section: Existing In Vivo Infection Modelsmentioning

confidence: 99%

See 1 more Smart Citation

Corneal Infection Models: Tools to Investigate the Role of Biofilms in Bacterial Keratitis

Urwin

Okurowska

Crowther

et al. 2020

Cells

View full text Add to dashboard Cite

Bacterial keratitis is a corneal infection which may cause visual impairment or even loss of the infected eye. It remains a major cause of blindness in the developing world. Staphylococcus aureus and Pseudomonas aeruginosa are common causative agents and these bacterial species are known to colonise the corneal surface as biofilm populations. Biofilms are complex bacterial communities encased in an extracellular polymeric matrix and are notoriously difficult to eradicate once established. Biofilm bacteria exhibit different phenotypic characteristics from their planktonic counterparts, including an increased resistance to antibiotics and the host immune response. Therefore, understanding the role of biofilms will be essential in the development of new ophthalmic antimicrobials. A brief overview of biofilm-specific resistance mechanisms is provided, but this is a highly multifactorial and rapidly expanding field that warrants further research. Progression in this field is dependent on the development of suitable biofilm models that acknowledge the complexity of the ocular environment. Abiotic models of biofilm formation (where biofilms are studied on non-living surfaces) currently dominate the literature, but co-culture infection models are beginning to emerge. In vitro, ex vivo and in vivo corneal infection models have now been reported which use a variety of different experimental techniques and animal models. In this review, we will discuss existing corneal infection models and their application in the study of biofilms and host-pathogen interactions at the corneal surface.

show abstract

In silico and in vivo methods for designing antibiofilm agents against Pseudomonas aeruginosa and Staphylococcus aureus

Tamba,

Panicker,

Jobby

et al. 2023

Microbial Biofilms

View full text Add to dashboard Cite

Microscopic characterization of biofilm in mixed keratitis in a novel murine model

Cited by 9 publications

References 39 publications

Purpureocillium roseum sp. nov. A new ocular pathogen for humans and mice resistant to antifungals

Purpureocillium roseum sp. nov. A new ocular pathogen for humans and mice resistant to antifungals

Corneal Infection Models: Tools to Investigate the Role of Biofilms in Bacterial Keratitis

In silico and in vivo methods for designing antibiofilm agents against Pseudomonas aeruginosa and Staphylococcus aureus

Contact Info

Product

Resources

About