Microscopic analysis of the inhibition of staphylococcal biofilm formation by Escherichia coli and the disruption of preformed staphylococcal biofilm by bacteriophage

Abstract

The formation of bacterial biofilms is a severely encountered problem in clinical and industrial settings. Most of the naturally occurring bacterial strains are capable of forming mono or mixed biofilms. In this study, we evaluated the potentiality of three clinically relevant species in forming mono and mixed biofilms over glass surface. In addition, we also appraised the efficiency of bacteriophages in alleviating preformed mono and mixed biofilm. Our initial study focused on the ability of Escherichia coli, Staphylococcus aureus, and Pseudomonas aeruginosa in forming biofilm on glass cover slip. All the three strains were able to form mono biofilm, although at varying intensities. Interestingly, E. coli inhibited the formation of S. aureus biofilm in a mixed culture. Specific bacteriophages ɸ44AHJD and ɸX174 completely disrupted S. aureus and E. coli preformed biofilm structure after 72 hr of incubation. However, addition of either of the bacteriophage to the mixed E. coli–S. aureus promoted the formation of biofilm by the alternate strain that was not affected by the phage. Our findings elicit the potentiality of common bacterial strains in forming biofilms on smooth glass surface. In addition, these results are very promising for the development of effective drugs using intact bacteriophages for the removal of complicated bacterial biofilms formed in clinically relevant glass surfaces. The observations further complemented the earlier finding of competitive inhibition of S. aureus biofilm development by E. coli.

Bacteriophage-Mediated Control of Biofilm: A Promising New Dawn for the Future

“…Phage therapy has since then proven to be effective in eradicating biofilm secreted by various bacterial strains, while its studies in clinical settings are also extensive. Amongst them is the eradication of biofilms on the surfaces of medical devices, such as prostheses and catheters ( Morris et al, 2019 ; Cano et al, 2021 ; Manoharadas et al, 2021 ). For instance, Morris et al assessed the anti-biofilm activity of phage toward prosthesis-related infections caused by S. aureus .…”