Microscopic Analysis of Neurospora ropy Mutants Defective in Nuclear Distribution

Minke, Peter F.; Lee, In Hyung; Plamann, Michael

doi:10.1006/fgbi.1999.1160

Cited by 61 publications

(48 citation statements)

References 42 publications

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“…The migration and positioning of nuclei within fungal hyphae have been reported to be regulated by the microtubuleassociated motor protein dynein and its activator, dynactin (9,38,41,49). Our analysis of three ropy mutants that were defective in different components of the cytoplasmic dynein/ dynactin complex confirmed its role in nuclear positioning because the nuclei of these mutants had a strong tendency to remain in the conidium after germination.…”

Section: Discussionsupporting

confidence: 65%

Nuclear Dynamics, Mitosis, and the Cytoskeleton during the Early Stages of Colony Initiation in Neurospora crassa

et al. 2010

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Neurospora crassa macroconidia form germ tubes that are involved in colony establishment and conidial anastomosis tubes (CATs) that fuse to form interconnected networks of conidial germlings. Nuclear and cytoskeletal behaviors were analyzed in macroconidia, germ tubes, and CATs in strains that expressed fluorescently labeled proteins. Heterokaryons formed by CAT fusion provided a rapid method for the imaging of multiple labeled fusion proteins and minimized the potential risk of overexpression artifacts. Mitosis occurred more slowly in nongerminated macroconidia (1.0 to 1.5 h) than in germ tubes (16 to 20 min). The nucleoporin SON-1 was not released from the nuclear envelope during mitosis, which suggests that N. crassa exhibits a form of "closed mitosis." During CAT homing, nuclei did not enter CATs, and mitosis was arrested. Benomyl treatment showed that CAT induction, homing, fusion, as well as nuclear migration through fused CATs do not require microtubules or mitosis. Three ropy mutants (ro-1, ro-3, and ro-11) defective in the dynein/dynactin microtubule motor were impaired in nuclear positioning, but nuclei still migrated through fused CATs. Latrunculin B treatment, imaging of F-actin in living cells using Lifeact-red fluorescent protein (RFP), and analysis of mutants defective in the Arp2/3 complex demonstrated that actin plays important roles in CAT fusion.

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Section: Discussionsupporting

confidence: 65%

Nuclear Dynamics, Mitosis, and the Cytoskeleton during the Early Stages of Colony Initiation in Neurospora crassa

et al. 2010

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“…We found that moving nuclei travel into the direction of the SPB and nuclei often adopt a tear-drop shape. This has been observed before in Nectria haematococca and Neurospora crassa (Inoue et al, 1998;Minke et al, 1999). The use of GFP-tagged MTs allowed us to investigate the role of MTs in this process.…”

Section: Mt-dependent Nuclear Movementsupporting

confidence: 59%

Role of the spindle-pole-body protein ApsB and the cortex protein ApsA in microtubule organization and nuclear migration in Aspergillus nidulans

Veith

Scherr

Efimov

et al. 2005

Journal of Cell Science

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Nuclear migration and positioning in Aspergillus nidulans depend on microtubules, the microtubule-dependent motor protein dynein, and auxiliary proteins, two of which are ApsA and ApsB. In apsA and apsB mutants nuclei are clustered and show various kinds of nuclear navigation defects, although nuclear migration itself is still possible. We studied the role of several components involved in nuclear migration through in vivo fluorescence microscopy using fluorescent-protein tagging. Because ApsA localizes to the cell cortex and mitotic spindles were immobile in apsA mutants, we suggest that astral microtubule-cortex interactions are necessary for oscillation and movement of mitotic spindles along hyphae, but not for post-mitotic nuclear migration. Mutation of apsA resulted in longer and curved microtubules and displayed synthetic lethality in combination with the conventional kinesin mutation ΔkinA. By contrast, ApsB localized to spindle-pole bodies (the fungal centrosome), to septa and to spots moving rapidly along microtubules. The number of cytoplasmic microtubules was reduced in apsB mutants in comparison to the wild type, indicating that cytoplasmic microtubule nucleation was affected, whereas mitotic spindle formation appeared normal. Mutation of apsB suppressed dynein null mutants, whereas apsA mutation had no effect. We suggest that nuclear positioning defects in the apsA and apsB mutants are due to different effects on microtbule organisation. A model of spindle-pole body led nuclear migration and the roles of dynein and microtubules are discussed.

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“…An alternative is parasynchronous mitosis, a wave of nuclear divisions that travels along the hypha or hyphal compartment, which has been extensively studied in A. nidulans (15). Finally, nuclei of the same compartment can undergo asynchronous mitosis independently of their neighboring nuclei, as has been observed in N. crassa and A. gossypii (16,17; for a review, see reference 18). Colletotrichum lindemuthianum provides an interesting case, in which different mitotic patterns at different developmental stages were observed.…”

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confidence: 99%

Dynamics of the Establishment of Multinucleate Compartments in Fusarium oxysporum

et al. 2015

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bNuclear dynamics can vary widely between fungal species and between stages of development of fungal colonies. Here we compared nuclear dynamics and mitotic patterns between germlings and mature hyphae in Fusarium oxysporum. Using fluorescently labeled nuclei and live-cell imaging, we show that F. oxysporum is subject to a developmental transition from a uninucleate to a multinucleate state after completion of colony initiation. We observed a special type of hypha that exhibits a higher growth rate, possibly acting as a nutrient scout. The higher growth rate is associated with a higher nuclear count and mitotic waves involving 2 to 6 nuclei in the apical compartment. Further, we found that dormant nuclei of intercalary compartments can reenter the mitotic cycle, resulting in multinucleate compartments with up to 18 nuclei in a single compartment.

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Microscopic Analysis of Neurospora ropy Mutants Defective in Nuclear Distribution

Cited by 61 publications

References 42 publications

Nuclear Dynamics, Mitosis, and the Cytoskeleton during the Early Stages of Colony Initiation in Neurospora crassa

Nuclear Dynamics, Mitosis, and the Cytoskeleton during the Early Stages of Colony Initiation in Neurospora crassa

Role of the spindle-pole-body protein ApsB and the cortex protein ApsA in microtubule organization and nuclear migration in Aspergillus nidulans

Dynamics of the Establishment of Multinucleate Compartments in Fusarium oxysporum

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