Microscale Surgery on Single Axons

Sretavan, David W.; Chang, Wesley C.; Hawkes, Elizabeth; Keller, Chris G.; Kliot, Michel

doi:10.1093/neurosurgery/57.4.635

Cited by 20 publications

(25 citation statements)

References 45 publications

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“…Artificially induced fusion can be used to investigate and treat different diseases, like diabetes123, regenerate axons of the central nerve system4, and produce cells with desired properties, such as in cell vaccines for cancer immunotherapy567. However, the first and most known application of cell fusion is production of monoclonal antibodies in hybridoma technology, where hybrid cell lines (hybridomas) are formed by fusing specific antibody-producing B lymphocytes with a myeloma (B lymphocyte cancer) cell line89.…”

mentioning

confidence: 99%

Cell electrofusion using nanosecond electric pulses

Rems

Ušaj

Kandušer

et al. 2013

Sci Rep

117

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Electrofusion is an efficient method for fusing cells using short-duration high-voltage electric pulses. However, electrofusion yields are very low when fusion partner cells differ considerably in their size, since the extent of electroporation (consequently membrane fusogenic state) with conventionally used microsecond pulses depends proportionally on the cell radius. We here propose a new and innovative approach to fuse cells with shorter, nanosecond (ns) pulses. Using numerical calculations we demonstrate that ns pulses can induce selective electroporation of the contact areas between cells (i.e. the target areas), regardless of the cell size. We then confirm experimentally on B16-F1 and CHO cell lines that electrofusion of cells with either equal or different size by using ns pulses is indeed feasible. Based on our results we expect that ns pulses can improve fusion yields in electrofusion of cells with different size, such as myeloma cells and B lymphocytes in hybridoma technology.

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mentioning

confidence: 99%

Cell electrofusion using nanosecond electric pulses

Rems

Ušaj

Kandušer

et al. 2013

Sci Rep

117

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“…We have designed an application that spans all four typical treatment phases of orthopaedic trauma surgery. As technical novelty, it enables true 3D surgical operation planning for orthopaedic trauma [8]. As conceptual novelty, it exemplifies the integration of surgical planning and hospital logistics.…”

Section: Discussionmentioning

confidence: 99%

“…Extreme microneurosurgery-operating at the near-nano or micron level on individual axons-has been demonstrated by Sretavan [8]. The device is a platform incorporating a 5-10 lm cutting blade, electrokinetic manipulation of the axons, and electrofusion of the cut axons.…”

Section: Robotic Nanoneurosurgerymentioning

confidence: 99%

“…By means of a formal notation they were able to analyze neurosurgical procedure steps. Strauß et al [8] analyzed the benefits of using navigation in FESS interventions. The objective of this paper is the example analysis of microsurgical lumbar discectomies with the ICCAS Surgical Workflow Analysis-suite (SWAN-suite) software framework [3,4] as a specialized instrument for acquisition support of data for SPM.…”

Section: Introductionmentioning

confidence: 99%

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Surgical PACS and the Digital Operating Room

2007

Int J CARS

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The contribution shows how the mission critical processes and the relevant IT-driven systems has been integrated in a minimally invasive surgery. The paper differentiates purpose, methods, results and conclusions. Purpose was to show that integration is a key aspect in the optimization of the medical treatment. Two main aspects of integration are topic of the article. The first is the integration of the OR itself, second is the integration of the OR into the hospital IT infrastructure. The results are proven by a vast data set collected over the last years in any case before and after the described technical intervention. Keywords Health care management AE E-health AE Minimally invasive surgery AE Process optimization AE Process integration 1. Purpose The application of novel technologies in medicine is often made responsible for being a substantial cause for expenditures in healthcare. This contribution focuses on the integration of systems and technologies in interventional units of a hospital and shows, using a real scenario example, that the efficient use of novel technologies is possible. This contribution will demonstrate a highly engineered operating room for minimally invasive surgery in which all devices are completely integrated within themselves. The main emphasis, however, is the integration of cutting edge technology of an operating room with all relevant software systems utilized in a hospital outside of the OR department. The authors distinguish between varied relevant processes, along which the systems are integrated. They will describe the integrated OR on the one hand and, on the other hand, the integration of the OR into the processes outside of the OR. The contribution uses extensive data to prove that the use of the integrated OR and the integration into further software systems makes the efficient application of expensive medical technology possible. Methods The scenarioIn an existing hospital for minimally invasive surgery currently more than 3,100 minimally invasive procedures are conducted per annum in a single operating room. All technologies, methods and results described here have been implemented and are in use in the professional daily routine of the hospital. 2.2. Differentiation between primary and secondary processes The scenario differentiates between primary and secondary processes, whereby primary processes are always of a medical nature (operations) and secondary processes are divided into medical and non-medical processes. Medical secondary processes include, amongst others, diagnosis and in-patient-stay. Non-medical secondary processes are, for example, treatment documentation and patient logistics. All processes concerning patient's treatment are described as a model using UML diagrams. The modelling was done prior to the integration of the systems and is presented in two variations: The respective processes not integrated and the integrated processes. The example shows non-medical secondary processes of intra-operative documentation on the most modeled degree of abstractio...

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“…A nanoknife with a 40 nanometer diameter has been developed and found to be effective for axon surgery 42 . The use of dielectrophoresis, which involves the use of electrical fields to manipulate polarizable objects in space, has been found to be effective in achieving controlled movement of axons within a surgical field 43 . Following controlled transection of axons and maneuvering them into position using dielectrophoresis, fusion between the two ends can be induced via electrofusion 44 , polyethylene glycol 45 , or laser-induced cell fusion 46 , amongst other methods.…”

Section: Neurosurgerymentioning

confidence: 99%

Nanorobotic Applications in Medicine: Current Proposals and Designs

Saadeh¹,

Vyas²

2014

Amer J Robot Surg

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Advances in technology have increased our ability to manipulate the world around us on an ever-decreasing scale. Nanotechnologies are rapidly emerging within the realm of medicine, and this subfield has been termed nanomedicine. Use of nanoparticle technology has become familiar and increasingly commonplace, especially with pharmaceutical technology. An exciting and promising area of nanotechnological development is the building of nanorobots, which are devices with components manufactured on the nanoscale. This area of study is replete with potential applications, many of which are currently being researched and developed. The goal of this paper is to give an introduction to the emerging field of nanorobotics within medicine, and provide a review of the emerging applications of nanorobotics to fields ranging from neurosurgery to dentistry.

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Microscale Surgery on Single Axons

Abstract: It is hoped that each advance in axon repair technology will spur additional research to provide us with a comprehensive understanding on how best to pursue neurosurgical intervention at the microscale.

Cited by 20 publications

References 45 publications

Cell electrofusion using nanosecond electric pulses

Cell electrofusion using nanosecond electric pulses

Surgical PACS and the Digital Operating Room

Nanorobotic Applications in Medicine: Current Proposals and Designs

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