Microscale supercritical fluid extraction combined with supercritical fluid chromatography and proton-transfer-reaction ionization time-of-flight mass spectrometry for a magnitude lower limit of quantitation of lipophilic compounds

Hondo, Toshinobu; Ota, Chihiro; Miyake, Yumi; Furutani, Hiroshi; Toyoda, Michisato

doi:10.1016/j.chroma.2022.463495

Cited by 4 publications

(2 citation statements)

References 25 publications

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“…As previously reported 7,8) , SFE-PTR MS can also detect nonvolatile small molecules with high sensitivity, at least up to m/z 1000. The addition of chromatography separation to SFE-PTR MS improves the analytical performance, such as isomer peak resolution and better detection sensitivity.…”

Section: Introductionsupporting

confidence: 68%

“…Although it is essentially a water ion chemical ionization, we have called it proton transfer reaction (PTR) ionization mass spectrometry (MS). 7,8) Supercritical fluid extraction (SFE) is a rapid and safe method for extracting lipophilic compounds from complex sample matrices. When applicable, SFE can also be used to extract polar molecules employing an entrainer.…”

Section: Introductionmentioning

confidence: 99%

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Rapid Analysis of α-Tocopherol and Its Oxidation Products Using Supercritical Carbon Dioxide and Proton Transfer Reaction Ionization Mass Spectrometry

et al. 2022

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We have developed a rapid and sensitive analytical method for α-tocopherol and its oxidative products by combining online hyphenation of supercritical fluid extraction-supercritical fluid chromatography (SFC) with proton transfer reaction (PTR) ionization mass spectrometry (MS). α-Tocopherol is a well-known antioxidant that plays a vital role in the antioxidant defense system in plant cells. However, studies on the cellular mechanisms of α-tocopherol have been limited owing to the lack of a rapid analytical method, which limits the comparison of plant cells incubated in various conditions. Additionally, complex sample preparation and long chromatography separation times are required. Moreover, the majority of the involved molecules are a combination of isomers, which must be separated before applying tandem MS. α-Tocopherol produces the α-tocopheroxyl radical in the first step of its antioxidant function; another ion with the same mass may also be generated from the source. SFC separation effectively distinguished the observed ions from their oxidative products in the sample and those produced during the ionization reaction process. This method enabled the measurement of α-tocopherol and its oxidative products such as α-tocopheroxyl radical and αtocopheryl quinone in approximately 3 min per sample, including the time required for sample preparation.

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Section: Introductionsupporting

confidence: 68%

Section: Introductionmentioning

confidence: 99%

Rapid Analysis of α-Tocopherol and Its Oxidation Products Using Supercritical Carbon Dioxide and Proton Transfer Reaction Ionization Mass Spectrometry

et al. 2022

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Fundamentals of supercritical fluid extraction

Santana,

Meireles

2024

Reference Module in Chemistry, Molecular Sciences and Chemical Engineering

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Attempts to Detect Lipid Metabolites from a Single Cell Using Proton-Transfer-Reaction Mass Spectrometry Coupled with Micro-Scale Supercritical Fluid Extraction: A Preliminary Study

Hondo¹,

Ota

Nakatani

et al. 2022

Mass Spectrometry

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Proton-transfer-reaction (PTR) mass spectrometry (MS), a widely used method for detecting tracelevels of volatile organic compounds in gaseous samples, can also be used for the analysis of small nonvolatile molecules by using supercritical fluid as a transporter for the molecules. Supercritical fluid extraction (SFE) is a method that permits lipophilic compounds to be rapidly and selectively extracted from complex matrices. The combination of the high sensitivity of PTR MS with the SFE is a potentially novel method for analyzing small molecules in a single cell, particularly for the analysis of lipophilic compounds. We preliminarily evaluated this method for analyzing the components of a single HeLa cell that is fixed on a stainless steel frit and is then directly introduces the SFE extracts into the PTR MS. A total of 200/90 ions were observed in positive/negative ion mode time-of-flight mass spectra, and the masses of 11/10 ions could be matched to chemical formulae obtained from the LipidMaps

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Microscale supercritical fluid extraction combined with supercritical fluid chromatography and proton-transfer-reaction ionization time-of-flight mass spectrometry for a magnitude lower limit of quantitation of lipophilic compounds

Cited by 4 publications

References 25 publications

Rapid Analysis of α-Tocopherol and Its Oxidation Products Using Supercritical Carbon Dioxide and Proton Transfer Reaction Ionization Mass Spectrometry

Rapid Analysis of α-Tocopherol and Its Oxidation Products Using Supercritical Carbon Dioxide and Proton Transfer Reaction Ionization Mass Spectrometry

Fundamentals of supercritical fluid extraction

Attempts to Detect Lipid Metabolites from a Single Cell Using Proton-Transfer-Reaction Mass Spectrometry Coupled with Micro-Scale Supercritical Fluid Extraction: A Preliminary Study

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