Microsatellites in Portuguese autochthonous horse breeds: usefulness for parentage testing

Abstract

The use of DNA technology for parentage testing is increasing every day. Most laboratories have improved their exclusion probabilities (PE) by the addition of DNA microsatellite loci to standard blood-typing results. The efficacy of each locus depends on the number of alleles detected and their frequencies in the breeds tested. Here we analyzed the usefulness of six microsatellites for routine parentage testing in three Portuguese autochthonous horse breeds: Lusitano, Sorraia and Garrano. The DNA loci analyzed -ASB2, HMS3, HMS7, HTG4, HTG10, and VHL20 -were chosen based upon the polymorphism detected in other breeds with Iberian horse influence. The estimated probability of exclusion of wrongly named parents (PE) was high, with values ranging from 88.5% to 99.6%.

Genetic diversity and population structure in Brazilian Mangalarga Marchador horses

“…Low genetic diversity was reported for the Spanish Purebred (0.452), Sorraia (0.486) and Uruguayan Creole (0.503) horse breeds, and higher values were found in Lusitano (0.735) and Garrano (0.788) horses. Intermediate values, similar to those reported here for the MM breed, were also described for Lippizan (0.663), Sable Island (0.626), and Anglo-Arab (0.66) horses (Kelly et al, 2002;Tozaki et al, 2003;Achmann et al, 2004;Luís et al, 2002Luís et al, , 2005Plante et al, 2007). Recently, a relatively high mean PIC (0.700) was reported for the Pantaneiro horse, an endangered Brazilian feral horse breed (Giacomoni et al, 2008 Table 2.…”

Genetic variability in local Brazilian horse lines using microsatellite markers

“…These values are identical to those found by Tozaki et al (2001) and higher than the ones found by Luis et al (2002) (88.5 and 99.6% knowing one or neither parent, respectively). In the breed analysis, Pantaneira showed the best results, with an exclusion probability of 99.91% knowing a single parent, and Campeira the worst results with a probability of 99.59%.…”

Genotyping in the Brazilian Criollo Horse Stud Book: resources and perspectives

“…Usually, alterations of genotypic proportions can be due to population substructuration (Waits et al, 2001;Luís et al, 2002;Dakin and Avise, 2004), linkage to traits under selection (Bowling et al, 1997;Dakin and Avise, 2004;Paredes et al, 2009), favoring environmental constraints (Diaz et al, 2002), differences in sex-linked selection intensity (Vila et al, 2001;Mirol et al, 2002;Luis et al, 2007a,b;Paredes et al, 2009), or mating between related individuals (Dakin and Avise, 2004;Paredes et al, 2009). In addition, other factors inherent to the assessed data, such as the non-sampling of females, constant migration of individuals, regional differences in environment, and selection objectives, should also be considered.…”

“…Such assays have wide acceptance among the entities responsible for keeping pedigree registries (Jamieson and Taylor, 1997;Tozaki et al, 2001;Jakabová, 2002;Luís et al, 2002;Lee and Cho, 2006). Genetic characterization is the first step in the conservation of breeds (Solis et al, 2005;Plante et al, 2007).…”