Microsatellites for butterfly conservation: historical challenges, current relevance, and a guide to implementation
Emily Heffernan,
Megan Barkdull,
Noah Brady
Abstract:We are nearing the 20-year anniversary of a groundbreaking paper which details how microsatellite marker development in Lepidoptera is “extremely difficult for no apparent reason.” How far have we come in these past 20 years? Microsatellites are still the marker of choice in many population genetics studies for their ease of use, high degrees of polymorphism, species-specificity, and low cost. The rise of next-generation sequencing technologies (e.g. 454, Illumina, PacBio, etc.) has greatly advanced our abilit… Show more
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